Restriction map for
pFN21AE1548
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 601 GACGTC GACGT^C
AccI 604 GTCTAC GT^[AC][GT]AC
AccII 163 CGCG CG^CG
AluI 353 AGCT AG^CT
AlwNI 562 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 23 GGATCC G^GATCC
BbrPI 616 CACGTG CAC^GTG
BbvCI 88 GCTGAGG CCTCAGC(-5/-2)
BbvI 369 GCAGC GCAGC(8/12)
BglI 249 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 72 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC
BsaAI 616 CACGTG [CT]AC^GT[GA]
BsaBI 457 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 482 TCCGGT [AT]^CCGG[AT]
BsmBI 599 GAGACG CGTCTC(1/5)
BsmFI 154 GGGAC GGGAC(10/14)
BstF5I 404 CATCC GGATG(2/0)
BstUI 163 CGCG CG^CG
BstXI 644 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 72 GCTGAGC GC^T[ACGT]AGC
CfoI 162 GCGC GCG^C
DraIII 324 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 660 GCGGG CCCGC(4/6)
FokI 404 CATCC GGATG(9/13)
HhaI 162 GCGC GCG^C
HinP1I 162 GCGC G^CGC
MamI 457 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 128 TTAA T^TAA
MvnI 163 CGCG CG^CG
NlaIII 135 CATG CATG^
NspI 134 GCATGT [GA]CATG^[CT]
PflMI 561 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 616 CACGTG CAC^GTG
PmlI 616 CACGTG CAC^GTG
PpuMI 304 AGGACCC [GA]G^G[AT]CC[CT]
SacI 352 GAGCTC GAGCT^C
ScaI 272 AGTACT AGT^ACT
SmaI 308 CCCGGG CCC^GGG
StuI 33 AGGCCT AGG^CCT
TaqI 215 TCGA T^CGA
TfiI 673 GAATC G^A[AT]TC
Tru9I 128 TTAA T^TAA
TseI 369 GCAGC G^C[AT]GC
TthHB8I 215 TCGA T^CGA
Van91I 561 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 246 GACGCC G[GA]^CG[CT]C
601 GACGTC
AvaII 305 GGACC G^G[AT]CC
554 GGACC
BsaHI 246 GACGCC G[GA]^CG[CT]C
601 GACGTC
BseRI 199 GAGGAG GAGGAG(10/8)
505 GAGGAG
BsgI 778 CTGCAC GTGCAG(16/14)
330 GTGCAG
BspMI 434 ACCTGC ACCTGC(4/8)
610 ACCTGC
BstYI 23 GGATCC [GA]^GATC[CT]
563 AGATCC
DraII 304 AGGACCC [GA]G^G[ACGT]CC[CT]
416 AGGCCCT
EcoO109I 304 AGGACCC [GA]G^G[ACGT]CC[CT]
416 AGGCCCT
HgaI 246 GACGC GACGC(5/10)
168 GCGTC
Hin1I 246 GACGCC G[GA]^CG[CT]C
601 GACGTC
MboII 9 GAAGA GAAGA(8/7)
728 TCTTC
MflI 23 GGATCC [GA]^GATC[CT]
563 AGATCC
MslI 78 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
645 CATCATCGTG
MspA1I 250 CCGCTG C[AC]G^C[GT]G
370 CAGCGG
TspRI 389 CAGTG CA[GC]TG[ACGT][ACGT]^
449 CAGTG
XhoII 23 GGATCC [GA]^GATC[CT]
563 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AlwI AspHI AvaI
BanII BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MnlI
MspI MvaI MwoI NciI NdeII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tsp45I