Restriction map for
pFN21AE1340
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AgeI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII BbrPI BclI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BssHII BstBI BstEII BstPI
ClaI DraI DraIII DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacI
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XhoI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1098 GTATAC GT^[AC][GT]AC
AflII 553 CTTAAG C^TTAAG
AocI 277 CCTGAGG CC^T[ACGT]AGG
AspHI 1144 GTGCTC G[AT]GC[AT]^C
AvrII 1416 CCTAGG C^CTAGG
BbsI 2341 GTCTTC GAAGAC(2/6)
BbvCI 1713 CCTCAGC CCTCAGC(-5/-2)
BfrI 553 CTTAAG C^TTAAG
BlnI 1416 CCTAGG C^CTAGG
BlpI 178 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 178 GCTGAGC GC^T[ACGT]AGC
BpuAI 2341 GTCTTC GAAGAC(2/6)
BsiHKAI 1144 GTGCTC G[AT]GC[AT]^C
BsmI 596 GCATTC GAATGC(1/-1)
BsrDI 1847 GCAATG GCAATG(2/0)
Bst1107I 1098 GTATAC GTA^TAC
BstZ17I 1098 GTATAC GTA^TAC
Bsu36I 277 CCTGAGG CC^T[ACGT]AGG
CelII 178 GCTGAGC GC^T[ACGT]AGC
CpoI 650 CGGACCG CG^G[AT]CCG
EarI 1269 CTCTTC CTCTTC(1/4)
Eco81I 277 CCTGAGG CC^T[ACGT]AGG
Ksp632I 1269 CTCTTC CTCTTC(1/4)
MluI 1118 ACGCGT A^CGCGT
MseI 554 TTAA T^TAA
RsrII 650 CGGACCG CG^G[AT]CCG
SapI 1268 GCTCTTC GCTCTTC(1/4)
SbfI 750 CCTGCAGG CCTGCA^GG
SmaI 1388 CCCGGG CCC^GGG
Sse8387I 750 CCTGCAGG CCTGCA^GG
Tru9I 554 TTAA T^TAA
Tsp509I 1009 AATT ^AATT
XbaI 1353 TCTAGA T^CTAGA
XcmI 776 CCACAGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1388 CCCGGG C^CCGGG
XmaI 1388 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AhdI 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2011 GACGTGTGGTC
ApaI 1630 GGGCCC GGGCC^C
1688 GGGCCC
AspEI 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2011 GACGTGTGGTC
AspI 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2230 GACAAGGTC
BalI 189 TGGCCA TGG^CCA
1434 TGGCCA
BamHI 2027 GGATCC G^GATCC
2368 GGATCC
BbeI 1694 GGCGCC GGCGC^C
1968 GGCGCC
BcgI 872 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1007 GCAATTCCATCG
BciVI 1823 GTATCC GTATCC(6/5)
2067 GTATCC
BglI 998 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1697 GCCCCCCAGGC
BglII 1356 AGATCT A^GATCT
2042 AGATCT
BsaAI 1445 TACGTG [CT]AC^GT[GA]
1639 TACGTG
BsaI 1551 GGTCTC GGTCTC(1/5)
1767 GGTCTC
BsmBI 1309 CGTCTC CGTCTC(1/5)
2247 CGTCTC
Bsp1407I 818 TGTACA T^GTACA
1994 TGTACA
BsrBI 1747 CCGCTC CCGCTC(-3/-3)
727 GAGCGG
BsrGI 818 TGTACA T^GTACA
1994 TGTACA
BstXI 776 CCACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1490 CCAGCACTGTGG
Eam1105I 385 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2011 GACGTGTGGTC
KasI 1694 GGCGCC G^GCGCC
1968 GGCGCC
KspI 654 CCGCGG CCGC^GG
1902 CCGCGG
MluNI 189 TGGCCA TGG^CCA
1434 TGGCCA
MscI 189 TGGCCA TGG^CCA
1434 TGGCCA
NaeI 1190 GCCGGC GCC^GGC
1339 GCCGGC
NarI 1694 GGCGCC GG^CGCC
1968 GGCGCC
NgoMIV 1190 GCCGGC G^CCGGC
1339 GCCGGC
NspI 477 GCATGC [GA]CATG^[CT]
1796 GCATGC
PflFI 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2230 GACAAGGTC
PflMI 456 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
542 CCATCCGCTGG
PvuII 518 CAGCTG CAG^CTG
2212 CAGCTG
SacII 654 CCGCGG CCGC^GG
1902 CCGCGG
SexAI 1547 ACCTGGT A^CC[AT]GGT
1781 ACCAGGT
SfoI 1694 GGCGCC GGC^GCC
1968 GGCGCC
SmlI 88 CTTGAG C^T[CT][GA]AG
553 CTTAAG
SphI 477 GCATGC GCATG^C
1796 GCATGC
SspBI 818 TGTACA T^GTACA
1994 TGTACA
TfiI 333 GAATC G^A[AT]TC
2335 GATTC
Tth111I 1546 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2230 GACAAGGTC
Van91I 456 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
542 CCATCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII