Restriction map for
pFN21AB5712
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# NO CUT ENZYMES ( 86 enzymes) #
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AccI AccIII AclI AflII AflIII
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII BalI
BamHI BcgI BciVI BfrI BglI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FspI HincII
HindII HindIII HpaI KspI MamI
MluI MluNI MroI MscI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SmaI SnaBI SpeI SspI SwaI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 961 GACGTC GACGT^C
AccII 1265 CGCG CG^CG
AcsI 632 AAATTC [GA]^AATT[CT]
AgeI 6 ACCGGT A^CCGGT
Alw44I 1858 GTGCAC G^TGCAC
ApaLI 1858 GTGCAC G^TGCAC
ApoI 632 AAATTC [GA]^AATT[CT]
AvrII 1773 CCTAGG C^CTAGG
BbeI 1271 GGCGCC GGCGC^C
BbrPI 526 CACGTG CAC^GTG
BclI 1386 TGATCA T^GATCA
BglII 1464 AGATCT A^GATCT
BlnI 1773 CCTAGG C^CTAGG
BsaAI 526 CACGTG [CT]AC^GT[GA]
BsiEI 1207 CGGCCG CG[GA][CT]^CG
BsmBI 719 GAGACG CGTCTC(1/5)
Bsp1407I 1427 TGTACA T^GTACA
BsrGI 1427 TGTACA T^GTACA
BstEII 1933 GGTGACC G^GT[ACGT]ACC
BstPI 1933 GGTGACC G^GT[ACGT]ACC
BstUI 1265 CGCG CG^CG
BstXI 525 CCACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 721 GACGAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1207 CGGCCG [CT]^GGCC[GA]
EagI 1207 CGGCCG C^GGCCG
EclXI 1207 CGGCCG C^GGCCG
Eco52I 1207 CGGCCG C^GGCCG
EcoO65I 1933 GGTGACC G^GT[ACGT]ACC
FbaI 1386 TGATCA T^GATCA
FseI 1204 GGCCGGCC GGCCGG^CC
KasI 1271 GGCGCC G^GCGCC
MfeI 648 CAATTG C^AATTG
MseI 409 TTAA T^TAA
MunI 648 CAATTG C^AATTG
MvnI 1265 CGCG CG^CG
NarI 1271 GGCGCC GG^CGCC
NcoI 1641 CCATGG C^CATGG
PinAI 6 ACCGGT A^CCGGT
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PshAI 1123 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2101 GAAGAGC GCTCTTC(1/4)
SbfI 66 CCTGCAGG CCTGCA^GG
SfaNI 1499 GCATC GCATC(5/9)
SfoI 1271 GGCGCC GGC^GCC
SgrAI 1177 CGCCGGCG C[GA]^CCGG[CT]G
SphI 424 GCATGC GCATG^C
Sse8387I 66 CCTGCAGG CCTGCA^GG
SspBI 1427 TGTACA T^GTACA
StuI 1155 AGGCCT AGG^CCT
TfiI 1114 GAATC G^A[AT]TC
Tru9I 409 TTAA T^TAA
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 392 GGTACC G^GTACC
1313 GGTACC
AocI 510 CCTGAGG CC^T[ACGT]AGG
883 CCTCAGG
Asp718I 392 GGTACC G^GTACC
1313 GGTACC
BsaWI 6 ACCGGT [AT]^CCGG[AT]
858 ACCGGA
BsgI 338 GTGCAG GTGCAG(16/14)
604 GTGCAG
BstAPI 1860 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1902 GCAGGAGCTGC
Bsu36I 510 CCTGAGG CC^T[ACGT]AGG
883 CCTCAGG
DraIII 658 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1861 CACCGTGTG
DsaI 1641 CCATGG C^C[GA][CT]GG
1684 CCACGG
Eco81I 510 CCTGAGG CC^T[ACGT]AGG
883 CCTCAGG
EcoNI 243 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1791 CCTGACCCAGG
HaeII 1271 GGCGCC [GA]GCGC^[CT]
1872 GGCGCT
KpnI 392 GGTACC GGTAC^C
1313 GGTACC
MaeII 527 ACGT A^CGT
962 ACGT
MaeIII 1217 GTGAC ^GT[ACGT]AC
1934 GTGAC
NaeI 1178 GCCGGC GCC^GGC
1205 GCCGGC
NgoMIV 1178 GCCGGC G^CCGGC
1205 GCCGGC
NspI 424 GCATGC [GA]CATG^[CT]
1472 GCATGT
PaeR7I 136 CTCGAG C^TCGAG
595 CTCGAG
TaqI 137 TCGA T^CGA
596 TCGA
Tsp45I 1217 GTGAC ^GT[GC]AC
1934 GTGAC
Tsp509I 633 AATT ^AATT
649 AATT
TthHB8I 137 TCGA T^CGA
596 TCGA
XcmI 566 CCAAGGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1361 CCAACTATAGTCTGG
XhoI 136 CTCGAG C^TCGAG
595 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TseI TspRI XhoII