Restriction map for
pFN21AE6149
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tsp509I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 102 CGCG CG^CG
AseI 370 ATTAAT AT^TAAT
AsnI 370 ATTAAT AT^TAAT
AvaII 56 GGACC G^G[AT]CC
BanII 34 GAGCCC G[GA]GC[CT]^C
BbsI 237 GAAGAC GAAGAC(2/6)
BbvCI 276 CCTCAGC CCTCAGC(-5/-2)
BbvI 5 GCTGC GCAGC(8/12)
BpuAI 237 GAAGAC GAAGAC(2/6)
BsaI 250 GAGACC GGTCTC(1/5)
BsgI 151 CTGCAC GTGCAG(16/14)
BsmI 347 GAATGC GAATGC(1/-1)
BsrI 255 CCAGT ACTGG(1/-1)
BstUI 102 CGCG CG^CG
DpnII 200 GATC ^GATC
DraII 285 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 285 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 112 AGCGCC [GA]GCGC^[CT]
HgaI 100 GACGC GACGC(5/10)
HincII 430 GTCAAC GT[CT]^[GA]AC
HindII 430 GTCAAC GT[CT]^[GA]AC
MboI 200 GATC ^GATC
MseI 371 TTAA T^TAA
MspA1I 129 CAGCGG C[AC]G^C[GT]G
MvnI 102 CGCG CG^CG
MwoI 8 GCGGTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 200 GATC ^GATC
NlaIII 162 CATG CATG^
NspI 161 GCATGC [GA]CATG^[CT]
PflMI 390 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 94 GACTC GAGTC(4/5)
PshAI 400 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 370 ATTAAT AT^TAAT
Sau3AI 200 GATC ^GATC
SfcI 203 CTACAG C^T[GA][CT]AG
SmaI 59 CCCGGG CCC^GGG
SphI 161 GCATGC GCATG^C
Tru9I 371 TTAA T^TAA
TseI 5 GCTGC G^C[AT]GC
Tsp45I 364 GTCAC ^GT[GC]AC
TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 390 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 297 CCAGAAACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 52 AGCT AG^CT
280 AGCT
AvaI 59 CCCGGG C^[CT]CG[GA]G
156 CTCGGG
BsmAI 64 GAGAC GTCTC(1/5)
250 GAGAC
BsoBI 59 CCCGGG C^[CT]CG[GA]G
156 CTCGGG
BstF5I 387 CATCC GGATG(2/0)
24 GGATG
BstNI 77 CCTGG CC^[AT]GG
211 CCAGG
CfoI 103 GCGC GCG^C
113 GCGC
EcoRII 77 CCTGG ^CC[AT]GG
211 CCAGG
FauI 38 CCCGC CCCGC(4/6)
142 GCGGG
FokI 387 CATCC GGATG(9/13)
24 GGATG
HhaI 103 GCGC GCG^C
113 GCGC
HinP1I 103 GCGC G^CGC
113 GCGC
HphI 83 TCACC GGTGA(8/7)
365 TCACC
MaeIII 364 GTCAC ^GT[ACGT]AC
376 GTAAC
MvaI 77 CCTGG CC^[AT]GG
211 CCAGG
TaqI 32 TCGA T^CGA
408 TCGA
TfiI 70 GAATC G^A[AT]TC
413 GAATC
TthHB8I 32 TCGA T^CGA
408 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI Cac8I Cfr13I
DdeI Fnu4HI HaeIII HapII HinfI
HpaII ItaI MboII MnlI MspI
NciI NlaIV Sau96I ScrFI SfaNI