Restriction map for
pFN21AE6349
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MroI MslI MspA1I MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 308 GGTACC G^GTACC
AccII 950 CGCG CG^CG
AciI 951 GCGG CCGC(-3/-1)
AhdI 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 925 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 308 GGTACC G^GTACC
AspEI 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 672 TGGCCA TGG^CCA
BanI 308 GGTACC G^G[CT][GA]CC
BbsI 891 GTCTTC GAAGAC(2/6)
BciVI 755 GGATAC GTATCC(6/5)
BpuAI 891 GTCTTC GAAGAC(2/6)
BsaI 1027 GAGACC GGTCTC(1/5)
BseRI 1064 GAGGAG GAGGAG(10/8)
BsmFI 108 GTCCC GGGAC(10/14)
BsmI 767 GAATGC GAATGC(1/-1)
BspHI 477 TCATGA T^CATGA
BsrFI 116 ACCGGC [GA]^CCGG[CT]
BstAPI 1106 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 950 CGCG CG^CG
Cac8I 190 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 116 ACCGGC [GA]^CCGG[CT]
DraI 1053 TTTAAA TTT^AAA
EaeI 672 TGGCCA [CT]^GGCC[GA]
Eam1105I 19 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1017 GAAGAG CTCTTC(1/4)
EcoRI 367 GAATTC G^AATTC
HapII 117 CCGG C^CGG
HpaII 117 CCGG C^CGG
HphI 137 TCACC GGTGA(8/7)
KpnI 308 GGTACC GGTAC^C
Ksp632I 1017 GAAGAG CTCTTC(1/4)
MluNI 672 TGGCCA TGG^CCA
MscI 672 TGGCCA TGG^CCA
MspI 117 CCGG C^CGG
MvnI 950 CGCG CG^CG
NdeI 803 CATATG CA^TATG
PleI 889 GAGTC GAGTC(4/5)
RcaI 477 TCATGA T^CATGA
SspI 148 AATATT AAT^ATT
TaqI 197 TCGA T^CGA
TthHB8I 197 TCGA T^CGA
XcmI 402 CCATAACTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AfaI 309 GTAC GT^AC
750 GTAC
ApaI 848 GGGCCC GGGCC^C
1122 GGGCCC
BanII 848 GGGCCC G[GA]GC[CT]^C
1122 GGGCCC
BbvI 54 GCTGC GCAGC(8/12)
871 GCTGC
BglII 606 AGATCT A^GATCT
917 AGATCT
BspMI 37 GCAGGT ACCTGC(4/8)
966 GCAGGT
NcoI 14 CCATGG C^CATGG
642 CCATGG
PstI 554 CTGCAG CTGCA^G
830 CTGCAG
RsaI 309 GTAC GT^AC
750 GTAC
SfcI 554 CTGCAG C^T[GA][CT]AG
830 CTGCAG
TseI 54 GCTGC G^C[AT]GC
871 GCTGC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AvaII BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I Tsp509I
TspRI Van91I XhoII XmnI