Restriction map for
pFN21AE6239
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BbeI BbsI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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BbrPI 138 CACGTG CAC^GTG
BbvCI 66 GCTGAGG CCTCAGC(-5/-2)
BcgI 504 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 84 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 107 ACCGGA [AT]^CCGG[AT]
BseRI 238 GAGGAG GAGGAG(10/8)
BsmAI 193 GAGAC GTCTC(1/5)
BspMI 809 ACCTGC ACCTGC(4/8)
BsrDI 427 GCAATG GCAATG(2/0)
BstF5I 264 CATCC GGATG(2/0)
BstXI 665 CCACTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 386 CCATGG C^C[GA][CT]GG
FokI 264 CATCC GGATG(9/13)
HphI 681 TCACC GGTGA(8/7)
MamI 84 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 146 TTAA T^TAA
NcoI 386 CCATGG C^CATGG
NheI 6 GCTAGC G^CTAGC
PmaCI 138 CACGTG CAC^GTG
PmlI 138 CACGTG CAC^GTG
PstI 469 CTGCAG CTGCA^G
SapI 403 GAAGAGC GCTCTTC(1/4)
SmlI 739 CTCAAG C^T[CT][GA]AG
StuI 254 AGGCCT AGG^CCT
TaqI 734 TCGA T^CGA
Tru9I 146 TTAA T^TAA
TspRI 204 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 734 TCGA T^CGA
XcmI 584 CCACCTGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AviII 50 TGCGCA TGC^GCA
812 TGCGCA
BalI 86 TGGCCA TGG^CCA
605 TGGCCA
BfaI 7 CTAG C^TAG
134 CTAG
BsaAI 138 CACGTG [CT]AC^GT[GA]
717 TACGTG
BssSI 502 CACGAG CACGAG(-5/-1)
409 CTCGTG
DpnII 459 GATC ^GATC
477 GATC
EaeI 86 TGGCCA [CT]^GGCC[GA]
605 TGGCCA
EarI 190 GAAGAG CTCTTC(1/4)
403 GAAGAG
EcoT14I 257 CCTTGG C^C[AT][AT]GG
386 CCATGG
FspI 50 TGCGCA TGC^GCA
812 TGCGCA
HaeII 572 AGCGCC [GA]GCGC^[CT]
818 AGCGCC
HapII 108 CCGG C^CGG
360 CCGG
HpaII 108 CCGG C^CGG
360 CCGG
Ksp632I 190 GAAGAG CTCTTC(1/4)
403 GAAGAG
MaeI 7 CTAG C^TAG
134 CTAG
MaeII 139 ACGT A^CGT
718 ACGT
MaeIII 206 GTGAC ^GT[ACGT]AC
545 GTGAC
MboI 459 GATC ^GATC
477 GATC
MluNI 86 TGGCCA TGG^CCA
605 TGGCCA
MscI 86 TGGCCA TGG^CCA
605 TGGCCA
MslI 585 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
666 CACTTGCATG
MspI 108 CCGG C^CGG
360 CCGG
NdeII 459 GATC ^GATC
477 GATC
NspI 47 ACATGC [GA]CATG^[CT]
623 ACATGC
PflMI 210 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
381 CCAGCCCATGG
PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT]
773 AGGTCCT
Sau3AI 459 GATC ^GATC
477 GATC
StyI 257 CCTTGG C^C[AT][AT]GG
386 CCATGG
Tsp45I 206 GTGAC ^GT[GC]AC
545 GTGAC
Van91I 210 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
381 CCAGCCCATGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwNI ApaI AspHI
AvaII BanII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI MboII MnlI MvaI MwoI
NlaIII NlaIV PleI SacI Sau96I
ScrFI SexAI SfaNI SfcI TseI