Restriction map for
pFN21AE6444
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BalI BamHI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI EaeI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MflI MluI MluNI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SbfI
ScaI SfcI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1193 TCCGGA T^CCGGA
AcyI 223 GGCGCC G[GA]^CG[CT]C
AflIII 722 ACGTGT A^C[GA][CT]GT
AlwI 704 GGATC GGATC(4/5)
AocI 34 CCTGAGG CC^T[ACGT]AGG
Aor51HI 237 AGCGCT AGC^GCT
AviII 341 TGCGCA TGC^GCA
BbeI 223 GGCGCC GGCGC^C
BbrPI 721 CACGTG CAC^GTG
BsaAI 721 CACGTG [CT]AC^GT[GA]
BsaHI 223 GGCGCC G[GA]^CG[CT]C
BsaI 103 GAGACC GGTCTC(1/5)
BsaWI 1193 TCCGGA [AT]^CCGG[AT]
BseAI 1193 TCCGGA T^CCGGA
BsmI 1203 GCATTC GAATGC(1/-1)
BspEI 1193 TCCGGA T^CCGGA
BspMI 1023 GCAGGT ACCTGC(4/8)
BsrBI 597 CCGCTC CCGCTC(-3/-3)
Bsu36I 34 CCTGAGG CC^T[ACGT]AGG
DraIII 625 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 550 GACACGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 237 AGCGCT AGC^GCT
Eco81I 34 CCTGAGG CC^T[ACGT]AGG
FspI 341 TGCGCA TGC^GCA
Hin1I 223 GGCGCC G[GA]^CG[CT]C
KasI 223 GGCGCC G^GCGCC
KspI 89 CCGCGG CCGC^GG
MroI 1193 TCCGGA T^CCGGA
NaeI 400 GCCGGC GCC^GGC
NarI 223 GGCGCC GG^CGCC
NgoMIV 400 GCCGGC G^CCGGC
PflMI 1387 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 721 CACGTG CAC^GTG
PmlI 721 CACGTG CAC^GTG
PpuMI 1468 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 515 CAGCTG CAG^CTG
SacII 89 CCGCGG CCGC^GG
SapI 1312 GAAGAGC GCTCTTC(1/4)
SexAI 1523 ACCTGGT A^CC[AT]GGT
SfoI 223 GGCGCC GGC^GCC
Van91I 1387 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1005 CCAGGAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 372 GTAC GT^AC
877 GTAC
AhdI 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1520 GACACCTGGTC
AspEI 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1520 GACACCTGGTC
AvaI 203 CTCGGG C^[CT]CG[GA]G
1318 CCCGAG
BbsI 760 GAAGAC GAAGAC(2/6)
1009 GAAGAC
BbvCI 517 GCTGAGG CCTCAGC(-5/-2)
736 GCTGAGG
BcnI 273 CCCGG CC^[GC]GG
381 CCGGG
BpuAI 760 GAAGAC GAAGAC(2/6)
1009 GAAGAC
BsoBI 203 CTCGGG C^[CT]CG[GA]G
1318 CCCGAG
BsrDI 953 GCAATG GCAATG(2/0)
1493 GCAATG
BsrFI 80 GCCGGT [GA]^CCGG[CT]
400 GCCGGC
BsrI 842 ACTGG ACTGG(1/-1)
869 CCAGT
Cfr10I 80 GCCGGT [GA]^CCGG[CT]
400 GCCGGC
Eam1105I 1030 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1520 GACACCTGGTC
EarI 478 GAAGAG CTCTTC(1/4)
1312 GAAGAG
HaeII 223 GGCGCC [GA]GCGC^[CT]
237 AGCGCT
HgaI 139 GACGC GACGC(5/10)
211 GACGC
Ksp632I 478 GAAGAG CTCTTC(1/4)
1312 GAAGAG
NciI 273 CCCGG CC^[GC]GG
381 CCGGG
PleI 169 GACTC GAGTC(4/5)
1012 GACTC
RsaI 372 GTAC GT^AC
877 GTAC
StuI 38 AGGCCT AGG^CCT
740 AGGCCT
TfiI 459 GAATC G^A[AT]TC
973 GATTC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SmlI
TseI Tsp45I TspRI