Restriction map for
pFN21AE1357
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 111 CGCG CG^CG
AcsI 172 GAATTC [GA]^AATT[CT]
AcyI 20 GGCGCC G[GA]^CG[CT]C
AfaI 387 GTAC GT^AC
AlwNI 328 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 172 GAATTC [GA]^AATT[CT]
AvaII 134 GGACC G^G[AT]CC
BamHI 54 GGATCC G^GATCC
BanI 20 GGCGCC G^G[CT][GA]CC
BanII 77 GGGCTC G[GA]GC[CT]^C
BbeI 20 GGCGCC GGCGC^C
BbsI 306 GTCTTC GAAGAC(2/6)
BbvI 376 GCTGC GCAGC(8/12)
BcnI 9 CCCGG CC^[GC]GG
BlpI 379 GCTCAGC GC^T[ACGT]AGC
BmyI 77 GGGCTC G[AGT]GC[ACT]^C
BpmI 59 CTCCAG CTGGAG(16/14)
Bpu1102I 379 GCTCAGC GC^T[ACGT]AGC
BpuAI 306 GTCTTC GAAGAC(2/6)
BsaHI 20 GGCGCC G[GA]^CG[CT]C
BsaJI 8 CCCCGG C^C[ACGT][ACGT]GG
Bsp1286I 77 GGGCTC G[AGT]GC[ACT]^C
BstUI 111 CGCG CG^CG
BstYI 54 GGATCC [GA]^GATC[CT]
Cac8I 443 GCTGGC GC[ACGT]^[ACGT]GC
CelII 379 GCTCAGC GC^T[ACGT]AGC
CfoI 21 GCGC GCG^C
DpnII 55 GATC ^GATC
DraI 157 TTTAAA TTT^AAA
DraII 133 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 133 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 172 GAATTC G^AATTC
HaeII 20 GGCGCC [GA]GCGC^[CT]
HaeIII 125 GGCC GG^CC
HhaI 21 GCGC GCG^C
Hin1I 20 GGCGCC G[GA]^CG[CT]C
HinP1I 21 GCGC G^CGC
KasI 20 GGCGCC G^GCGCC
MboI 55 GATC ^GATC
MflI 54 GGATCC [GA]^GATC[CT]
MseI 158 TTAA T^TAA
MvnI 111 CGCG CG^CG
MwoI 21 GCGCCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 20 GGCGCC GG^CGCC
NciI 9 CCCGG CC^[GC]GG
NdeII 55 GATC ^GATC
NlaIII 27 CATG CATG^
PpuMI 133 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 382 CAGCTG CAG^CTG
RsaI 387 GTAC GT^AC
Sau3AI 55 GATC ^GATC
SfcI 203 CTACAG C^T[GA][CT]AG
SfoI 20 GGCGCC GGC^GCC
Tru9I 158 TTAA T^TAA
TseI 376 GCTGC G^C[AT]GC
Tsp509I 173 AATT ^AATT
TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 54 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 383 AGCT AG^CT
442 AGCT
AlwI 55 GATCC GGATC(4/5)
54 GGATC
BsmAI 217 GTCTC GTCTC(1/5)
330 GTCTC
BsmFI 32 GGGAC GGGAC(10/14)
133 GGGAC
BstNI 372 CCAGG CC^[AT]GG
390 CCAGG
Cfr13I 124 GGGCC G^G[ACGT]CC
134 GGACC
DdeI 221 CTAAG C^T[ACGT]AG
380 CTCAG
EcoRII 372 CCAGG ^CC[AT]GG
390 CCAGG
FauI 30 GCGGG CCCGC(4/6)
74 GCGGG
Fnu4HI 18 GCGGC GC^[ACGT]GC
376 GCTGC
HapII 10 CCGG C^CGG
65 CCGG
HinfI 68 GAGTC G^A[ACGT]TC
288 GAGTC
HpaII 10 CCGG C^CGG
65 CCGG
ItaI 18 GCGGC GC^[ACGT]GC
376 GCTGC
MboII 181 GAAGA GAAGA(8/7)
307 TCTTC
MslI 96 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
232 CATCCAAATG
MspA1I 72 CAGCGG C[AC]G^C[GT]G
382 CAGCTG
MspI 10 CCGG C^CGG
65 CCGG
MvaI 372 CCAGG CC^[AT]GG
390 CCAGG
PleI 68 GAGTC GAGTC(4/5)
288 GAGTC
Sau96I 124 GGGCC G^G[ACGT]CC
134 GGACC
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BsiYI BslI BssKI BstF5I
FokI MnlI NlaIV ScrFI