Restriction map for
pFN21AE2158
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MamI
MboI MfeI MflI MluI MseI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccIII 521 TCCGGA T^CCGGA
AciI 507 GCGG CCGC(-3/-1)
Alw44I 497 GTGCAC G^TGCAC
ApaLI 497 GTGCAC G^TGCAC
Asp700I 405 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 422 TGGCCA TGG^CCA
BcnI 269 CCCGG CC^[GC]GG
BglI 217 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 521 TCCGGA [AT]^CCGG[AT]
BseAI 521 TCCGGA T^CCGGA
BsmBI 300 CGTCTC CGTCTC(1/5)
BsmI 529 GCATTC GAATGC(1/-1)
BspEI 521 TCCGGA T^CCGGA
BsrI 461 CCAGT ACTGG(1/-1)
BssSI 261 CACGAG CACGAG(-5/-1)
DrdI 399 GACTCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 422 TGGCCA [CT]^GGCC[GA]
EcoRI 160 GAATTC G^AATTC
EcoT14I 570 CCTTGG C^C[AT][AT]GG
HinfI 399 GACTC G^A[ACGT]TC
MboII 588 TCTTC GAAGA(8/7)
MluNI 422 TGGCCA TGG^CCA
MroI 521 TCCGGA T^CCGGA
MscI 422 TGGCCA TGG^CCA
NciI 269 CCCGG CC^[GC]GG
PleI 399 GACTC GAGTC(4/5)
StyI 570 CCTTGG C^C[AT][AT]GG
Tsp45I 447 GTCAC ^GT[GC]AC
TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 405 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 13 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
202 CAGAAACTG
ApaI 146 GGGCCC GGGCC^C
172 GGGCCC
AspHI 97 GTGCTC G[AT]GC[AT]^C
497 GTGCAC
BanII 146 GGGCCC G[GA]GC[CT]^C
172 GGGCCC
BpmI 129 CTGGAG CTGGAG(16/14)
340 CTGGAG
BsiHKAI 97 GTGCTC G[AT]GC[AT]^C
497 GTGCAC
BsmAI 491 GAGAC GTCTC(1/5)
301 GTCTC
BstF5I 306 GGATG GGATG(2/0)
599 GGATG
DdeI 248 CTCAG C^T[ACGT]AG
311 CTGAG
DraII 145 GGGGCCC [GA]G^G[ACGT]CC[CT]
172 GGGCCCT
EcoO109I 145 GGGGCCC [GA]G^G[ACGT]CC[CT]
172 GGGCCCT
FokI 306 GGATG GGATG(9/13)
599 GGATG
HapII 270 CCGG C^CGG
522 CCGG
HpaII 270 CCGG C^CGG
522 CCGG
MaeII 299 ACGT A^CGT
545 ACGT
MaeIII 320 GTTAC ^GT[ACGT]AC
447 GTCAC
MspI 270 CCGG C^CGG
522 CCGG
PstI 277 CTGCAG CTGCA^G
292 CTGCAG
SfaNI 307 GATGC GCATC(5/9)
600 GATGC
SfcI 277 CTGCAG C^T[GA][CT]AG
292 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AcsI AluI ApoI BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I EcoRII Fnu4HI
HaeIII ItaI MnlI MvaI MwoI
NlaIV Sau96I ScrFI TseI Tsp509I