Restriction map for
pFN21AE5207
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# NO CUT ENZYMES ( 169 enzymes) #
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Acc65I AccII AciI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaJI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MscI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 346 GACGTC GACGT^C
AccI 148 GTCTAC GT^[AC][GT]AC
AccIII 425 TCCGGA T^CCGGA
AcyI 346 GACGTC G[GA]^CG[CT]C
AlwI 262 GATCC GGATC(4/5)
AlwNI 226 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 279 GAGCAC G[AT]GC[AT]^C
AvaII 123 GGTCC G^G[AT]CC
BanII 134 GAGCCC G[GA]GC[CT]^C
BbsI 23 GTCTTC GAAGAC(2/6)
BcnI 509 CCCGG CC^[GC]GG
BfaI 253 CTAG C^TAG
BpmI 160 CTCCAG CTGGAG(16/14)
BpuAI 23 GTCTTC GAAGAC(2/6)
BsaHI 346 GACGTC G[GA]^CG[CT]C
BsaI 327 GAGACC GGTCTC(1/5)
BsaWI 425 TCCGGA [AT]^CCGG[AT]
BseAI 425 TCCGGA T^CCGGA
BsgI 495 GTGCAG GTGCAG(16/14)
BsiHKAI 279 GAGCAC G[AT]GC[AT]^C
BspEI 425 TCCGGA T^CCGGA
BspHI 387 TCATGA T^CATGA
BsrDI 526 CATTGC GCAATG(2/0)
BssSI 131 CACGAG CACGAG(-5/-1)
BstNI 185 CCTGG CC^[AT]GG
BstYI 261 AGATCC [GA]^GATC[CT]
DdeI 323 CTGAG C^T[ACGT]AG
DpnII 262 GATC ^GATC
DraII 122 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 115 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 122 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 185 CCTGG ^CC[AT]GG
HgaI 315 GACGC GACGC(5/10)
Hin1I 346 GACGTC G[GA]^CG[CT]C
HphI 482 TCACC GGTGA(8/7)
MaeI 253 CTAG C^TAG
MaeIII 256 GTGAC ^GT[ACGT]AC
MboI 262 GATC ^GATC
MflI 261 AGATCC [GA]^GATC[CT]
MroI 425 TCCGGA T^CCGGA
MslI 388 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 185 CCTGG CC^[AT]GG
MwoI 136 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 509 CCCGG CC^[GC]GG
NdeII 262 GATC ^GATC
NlaIV 123 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 225 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 100 GACTC GAGTC(4/5)
PpuMI 122 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 387 TCATGA T^CATGA
Sau3AI 262 GATC ^GATC
SmlI 370 CTCAAG C^T[CT][GA]AG
Tsp45I 256 GTGAC ^GT[GC]AC
Van91I 225 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 175 CCAACCCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 261 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BmyI 134 GAGCCC G[AGT]GC[ACT]^C
279 GAGCAC
BsmAI 268 GAGAC GTCTC(1/5)
327 GAGAC
BsmFI 124 GTCCC GGGAC(10/14)
507 GTCCC
Bsp1286I 134 GAGCCC G[AGT]GC[ACT]^C
279 GAGCAC
BssKI 185 CCTGG ^CC[ACGT]GG
509 CCCGG
Cac8I 189 GCCTGC GC[ACGT]^[ACGT]GC
497 GCAGGC
Cfr13I 123 GGTCC G^G[ACGT]CC
247 GGGCC
MspA1I 449 CAGCTG C[AC]G^C[GT]G
502 CAGCTG
NlaIII 111 CATG CATG^
388 CATG
PvuII 449 CAGCTG CAG^CTG
502 CAGCTG
Sau96I 123 GGTCC G^G[ACGT]CC
247 GGGCC
ScrFI 185 CCTGG CC^[ACGT]GG
509 CCCGG
SfaNI 142 GATGC GCATC(5/9)
470 GCATC
TfiI 45 GATTC G^A[AT]TC
522 GAATC
TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^
486 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsrI
BstF5I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeII MboII
MnlI MspI TseI