Restriction map for
pFN21AE1423
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 531 GTCTAC GT^[AC][GT]AC
AcsI 502 GAATTC [GA]^AATT[CT]
AflIII 180 ACACGT A^C[GA][CT]GT
ApoI 502 GAATTC [GA]^AATT[CT]
AvaI 188 CCCGGG C^[CT]CG[GA]G
BamHI 362 GGATCC G^GATCC
BbrPI 22 CACGTG CAC^GTG
BfaI 649 CTAG C^TAG
BlpI 1131 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC
BsgI 1111 GTGCAG GTGCAG(16/14)
BsiEI 1142 CGACCG CG[GA][CT]^CG
BsmAI 703 GTCTC GTCTC(1/5)
BsmFI 134 GGGAC GGGAC(10/14)
BsoBI 188 CCCGGG C^[CT]CG[GA]G
BspMI 34 GCAGGT ACCTGC(4/8)
BsrBI 463 GAGCGG CCGCTC(-3/-3)
BssSI 847 CACGAG CACGAG(-5/-1)
BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1096 GGTGACC G^GT[ACGT]ACC
BstPI 1096 GGTGACC G^GT[ACGT]ACC
CelII 1131 GCTGAGC GC^T[ACGT]AGC
EarI 256 CTCTTC CTCTTC(1/4)
EcoO65I 1096 GGTGACC G^GT[ACGT]ACC
EcoRI 502 GAATTC G^AATTC
EcoT22I 9 ATGCAT ATGCA^T
FauI 1166 CCCGC CCCGC(4/6)
HaeII 659 AGCGCC [GA]GCGC^[CT]
Ksp632I 256 CTCTTC CTCTTC(1/4)
MaeI 649 CTAG C^TAG
MspA1I 853 CAGCTG C[AC]G^C[GT]G
NsiI 9 ATGCAT ATGCA^T
NspI 1127 GCATGC [GA]CATG^[CT]
PmaCI 22 CACGTG CAC^GTG
PmlI 22 CACGTG CAC^GTG
PstI 830 CTGCAG CTGCA^G
PvuII 853 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
SapI 255 GCTCTTC GCTCTTC(1/4)
SexAI 773 ACCTGGT A^CC[AT]GGT
SmaI 188 CCCGGG CCC^GGG
SphI 1127 GCATGC GCATG^C
StuI 1157 AGGCCT AGG^CCT
TfiI 87 GATTC G^A[AT]TC
XcmI 414 CCACAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 188 CCCGGG C^CCGGG
XmaI 188 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 384 GTGCAC G^TGCAC
1213 GTGCAC
ApaI 737 GGGCCC GGGCC^C
1163 GGGCCC
ApaLI 384 GTGCAC G^TGCAC
1213 GTGCAC
AvaII 750 GGACC G^G[AT]CC
1173 GGACC
BalI 806 TGGCCA TGG^CCA
1103 TGGCCA
BsaAI 22 CACGTG [CT]AC^GT[GA]
181 CACGTA
BsmI 7 GAATGC GAATGC(1/-1)
1019 GCATTC
BsrDI 993 CATTGC GCAATG(2/0)
50 GCAATG
BstXI 414 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
896 CCAACCAGATGG
DraII 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
EaeI 806 TGGCCA [CT]^GGCC[GA]
1103 TGGCCA
EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTGGAGAAGG
EcoO109I 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
EcoT14I 974 CCAAGG C^C[AT][AT]GG
1106 CCAAGG
HapII 189 CCGG C^CGG
921 CCGG
HincII 475 GTTGAC GT[CT]^[GA]AC
982 GTCAAC
HindII 475 GTTGAC GT[CT]^[GA]AC
982 GTCAAC
HpaII 189 CCGG C^CGG
921 CCGG
MaeII 23 ACGT A^CGT
182 ACGT
MluNI 806 TGGCCA TGG^CCA
1103 TGGCCA
MscI 806 TGGCCA TGG^CCA
1103 TGGCCA
MslI 1080 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1216 CACTGGTATG
MspI 189 CCGG C^CGG
921 CCGG
PleI 139 GACTC GAGTC(4/5)
1312 GACTC
SfcI 533 CTACAG C^T[GA][CT]AG
830 CTGCAG
StyI 974 CCAAGG C^C[AT][AT]GG
1106 CCAAGG
Tsp509I 503 AATT ^AATT
699 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I XhoII