##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AfaI          254  GTAC                 GT^AC
AvrII          31  CCTAGG               C^CTAGG
BbvI          172  GCAGC                GCAGC(8/12)
BfaI           32  CTAG                 C^TAG
BlnI           31  CCTAGG               C^CTAGG
BpmI          208  CTCCAG               CTGGAG(16/14)
BsaJI          31  CCTAGG               C^C[ACGT][ACGT]GG
BsgI          170  GTGCAG               GTGCAG(16/14)
BsiYI         304  CCAGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          304  CCAGAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         310  GCAGGT               ACCTGC(4/8)
BsrI          232  ACTGG                ACTGG(1/-1)
DraI          295  TTTAAA               TTT^AAA
EcoT14I        31  CCTAGG               C^C[AT][AT]GG
Fnu4HI        172  GCAGC                GC^[ACGT]GC
HphI          313  GGTGA                GGTGA(8/7)
ItaI          172  GCAGC                GC^[ACGT]GC
MaeI           32  CTAG                 C^TAG
MseI          296  TTAA                 T^TAA
MspA1I        173  CAGCTG               C[AC]G^C[GT]G
MwoI           54  GCCTCCTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PvuII         173  CAGCTG               CAG^CTG
RsaI          254  GTAC                 GT^AC
SapI           94  GAAGAGC              GCTCTTC(1/4)
StuI           28  AGGCCT               AGG^CCT
StyI           31  CCTAGG               C^C[AT][AT]GG
Tru9I         296  TTAA                 T^TAA
TseI          172  GCAGC                G^C[AT]GC
TspRI          87  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BclI          144  TGATCA               T^GATCA
              162  TGATCA              
BssKI         195  CCTGG                ^CC[ACGT]GG
              210  CCAGG               
BstF5I        237  GGATG                GGATG(2/0)
              241  GGATG               
BstNI         195  CCTGG                CC^[AT]GG
              210  CCAGG               
DdeI           59  CTGAG                C^T[ACGT]AG
              176  CTGAG               
EcoRII        195  CCTGG                ^CC[AT]GG
              210  CCAGG               
FbaI          144  TGATCA               T^GATCA
              162  TGATCA              
FokI          237  GGATG                GGATG(9/13)
              241  GGATG               
HaeIII         29  GGCC                 GG^CC
               35  GGCC                
MnlI           55  CCTC                 CCTC(7/6)
               11  GAGG                
MvaI          195  CCTGG                CC^[AT]GG
              210  CCAGG               
ScrFI         195  CCTGG                CC^[ACGT]GG
              210  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        DpnII       EarI        HinfI       Ksp632I     
MboI        MboII       NdeII       Sau3AI      TfiI