Restriction map for
pFN21AE1436
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BfaI BfrI BglII
BlnI BsaHI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PleI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SexAI SfoI
SfuI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AfaI 34 GTAC GT^AC
AgeI 625 ACCGGT A^CCGGT
Alw44I 569 GTGCAC G^TGCAC
AlwI 12 GATCC GGATC(4/5)
ApaLI 569 GTGCAC G^TGCAC
Asp700I 660 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 569 GTGCAC G[AT]GC[AT]^C
BalI 150 TGGCCA TGG^CCA
BbrPI 430 CACGTG CAC^GTG
BbsI 664 GTCTTC GAAGAC(2/6)
BciVI 125 GTATCC GTATCC(6/5)
BclI 434 TGATCA T^GATCA
BlpI 84 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 84 GCTCAGC GC^T[ACGT]AGC
BpuAI 664 GTCTTC GAAGAC(2/6)
BsaAI 430 CACGTG [CT]AC^GT[GA]
BsaBI 6 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 625 ACCGGT [AT]^CCGG[AT]
BsgI 90 CTGCAC GTGCAG(16/14)
BsiHKAI 569 GTGCAC G[AT]GC[AT]^C
BspDI 64 ATCGAT AT^CGAT
BspMI 481 ACCTGC ACCTGC(4/8)
BsrFI 625 ACCGGT [GA]^CCGG[CT]
BstYI 11 AGATCC [GA]^GATC[CT]
CelII 84 GCTCAGC GC^T[ACGT]AGC
CfoI 402 GCGC GCG^C
Cfr10I 625 ACCGGT [GA]^CCGG[CT]
ClaI 64 ATCGAT AT^CGAT
DdeI 85 CTCAG C^T[ACGT]AG
EcoRV 67 GATATC GAT^ATC
FauI 464 CCCGC CCCGC(4/6)
FbaI 434 TGATCA T^GATCA
HhaI 402 GCGC GCG^C
HinP1I 402 GCGC G^CGC
MamI 6 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 11 AGATCC [GA]^GATC[CT]
MluNI 150 TGGCCA TGG^CCA
MscI 150 TGGCCA TGG^CCA
MslI 438 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 87 CAGCTG C[AC]G^C[GT]G
PaeR7I 527 CTCGAG C^TCGAG
PinAI 625 ACCGGT A^CCGGT
PmaCI 430 CACGTG CAC^GTG
PmlI 430 CACGTG CAC^GTG
PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT]
PstI 670 CTGCAG CTGCA^G
PvuII 87 CAGCTG CAG^CTG
RsaI 34 GTAC GT^AC
SbfI 669 CCTGCAGG CCTGCA^GG
ScaI 33 AGTACT AGT^ACT
SfcI 670 CTGCAG C^T[GA][CT]AG
SgrAI 624 CACCGGTG C[GA]^CCGG[CT]G
SmaI 120 CCCGGG CCC^GGG
Sse8387I 669 CCTGCAGG CCTGCA^GG
Tsp509I 454 AATT ^AATT
TspRI 468 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 527 CTCGAG C^TCGAG
XhoII 11 AGATCC [GA]^GATC[CT]
XmaCI 120 CCCGGG C^CCGGG
XmaI 120 CCCGGG C^CCGGG
XmnI 660 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 420 CCGC CCGC(-3/-1)
465 CCGC
AvaI 120 CCCGGG C^[CT]CG[GA]G
527 CTCGAG
BanI 489 GGTGCC G^G[CT][GA]CC
709 GGTGCC
BbvI 23 GCAGC GCAGC(8/12)
89 GCTGC
BsoBI 120 CCCGGG C^[CT]CG[GA]G
527 CTCGAG
BsrI 696 ACTGG ACTGG(1/-1)
31 CCAGT
BstEII 582 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
BstPI 582 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
DpnII 12 GATC ^GATC
435 GATC
EaeI 150 TGGCCA [CT]^GGCC[GA]
558 TGGCCG
EcoO65I 582 GGTCACC G^GT[ACGT]ACC
618 GGTCACC
Fnu4HI 23 GCAGC GC^[ACGT]GC
89 GCTGC
ItaI 23 GCAGC GC^[ACGT]GC
89 GCTGC
MboI 12 GATC ^GATC
435 GATC
MboII 9 GAAGA GAAGA(8/7)
665 TCTTC
NdeII 12 GATC ^GATC
435 GATC
Sau3AI 12 GATC ^GATC
435 GATC
SmlI 527 CTCGAG C^T[CT][GA]AG
640 CTCAAG
TseI 23 GCAGC G^C[AT]GC
89 GCTGC
XcmI 272 CCAAGGGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
623 CCACCGGTGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AluI ApaI AvaII BanII BcnI
BglI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DraII DsaI EcoO109I EcoRII EcoT14I
FokI HaeIII HapII HinfI HpaII
HphI MaeII MaeIII MnlI MspI
MvaI MwoI NciI NcoI NlaIII
NlaIV Sau96I ScrFI SfaNI SfiI
StyI TaqI TfiI Tsp45I TthHB8I