Restriction map for
pFN21AE6351
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbrPI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MaeI
MaeII MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SbfI
ScaI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 524 GGTACC G^GTACC
AcsI 469 GAATTT [GA]^AATT[CT]
AlwNI 736 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1051 CCTAAGG CC^T[ACGT]AGG
ApoI 469 GAATTT [GA]^AATT[CT]
Asp718I 524 GGTACC G^GTACC
AspHI 592 GAGCTC G[AT]GC[AT]^C
AvaI 85 CCCGAG C^[CT]CG[GA]G
BamHI 1056 GGATCC G^GATCC
BbeI 148 GGCGCC GGCGC^C
BbsI 658 GTCTTC GAAGAC(2/6)
BcgI 1096 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 216 CCGGG CC^[GC]GG
BlpI 943 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 943 GCTCAGC GC^T[ACGT]AGC
BpuAI 658 GTCTTC GAAGAC(2/6)
BseRI 1039 GAGGAG GAGGAG(10/8)
BsiEI 70 CGGCCG CG[GA][CT]^CG
BsiHKAI 592 GAGCTC G[AT]GC[AT]^C
BsiWI 162 CGTACG C^GTACG
BsmI 183 GAATGC GAATGC(1/-1)
BsoBI 85 CCCGAG C^[CT]CG[GA]G
BspHI 371 TCATGA T^CATGA
BspMI 527 ACCTGC ACCTGC(4/8)
BsrDI 816 CATTGC GCAATG(2/0)
BstAPI 617 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1051 CCTAAGG CC^T[ACGT]AGG
CelII 943 GCTCAGC GC^T[ACGT]AGC
DraII 482 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 174 CCGCGG C^C[GA][CT]GG
EagI 70 CGGCCG C^GGCCG
EclXI 70 CGGCCG C^GGCCG
Eco52I 70 CGGCCG C^GGCCG
Eco81I 1051 CCTAAGG CC^T[ACGT]AGG
EcoO109I 482 AGGACCT [GA]G^G[ACGT]CC[CT]
HapII 216 CCGG C^CGG
HgaI 518 GCGTC GACGC(5/10)
HpaII 216 CCGG C^CGG
KasI 148 GGCGCC G^GCGCC
KpnI 524 GGTACC GGTAC^C
KspI 174 CCGCGG CCGC^GG
MseI 581 TTAA T^TAA
MslI 675 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 216 CCGG C^CGG
NarI 148 GGCGCC GG^CGCC
NciI 216 CCGGG CC^[GC]GG
NotI 69 GCGGCCGC GC^GGCCGC
PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 371 TCATGA T^CATGA
SacI 592 GAGCTC GAGCT^C
SacII 174 CCGCGG CCGC^GG
SapI 420 GCTCTTC GCTCTTC(1/4)
SfoI 148 GGCGCC GGC^GCC
SphI 939 GCATGC GCATG^C
SspI 282 AATATT AAT^ATT
StuI 753 AGGCCT AGG^CCT
Tru9I 581 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 148 GGCGCC G[GA]^CG[CT]C
517 GGCGTC
AvaII 483 GGACC G^G[AT]CC
966 GGTCC
BalI 695 TGGCCA TGG^CCA
1004 TGGCCA
BanII 262 GGGCTC G[GA]GC[CT]^C
592 GAGCTC
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
531 GCTGAGG
BmyI 262 GGGCTC G[AGT]GC[ACT]^C
592 GAGCTC
BpmI 745 CTGGAG CTGGAG(16/14)
1114 CTGGAG
BsaHI 148 GGCGCC G[GA]^CG[CT]C
517 GGCGTC
BsgI 249 CTGCAC GTGCAG(16/14)
598 CTGCAC
Bsp1286I 262 GGGCTC G[AGT]GC[ACT]^C
592 GAGCTC
BsrI 542 ACTGG ACTGG(1/-1)
619 ACTGG
BstF5I 627 CATCC GGATG(2/0)
219 GGATG
BstXI 479 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
677 CCAATGTGATGG
EcoT14I 479 CCAAGG C^C[AT][AT]GG
1007 CCAAGG
FokI 627 CATCC GGATG(9/13)
219 GGATG
Hin1I 148 GGCGCC G[GA]^CG[CT]C
517 GGCGTC
HphI 687 GGTGA GGTGA(8/7)
209 TCACC
MluNI 695 TGGCCA TGG^CCA
1004 TGGCCA
MscI 695 TGGCCA TGG^CCA
1004 TGGCCA
NspI 194 ACATGC [GA]CATG^[CT]
939 GCATGC
PvuII 246 CAGCTG CAG^CTG
415 CAGCTG
SexAI 485 ACCTGGT A^CC[AT]GGT
962 ACCTGGT
StyI 479 CCAAGG C^C[AT][AT]GG
1007 CCAAGG
TfiI 338 GAATC G^A[AT]TC
1075 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BbvI BsaJI BsiYI BslI
BsmFI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
HaeII HaeIII HhaI HinP1I HinfI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII