Restriction map for
pFN21AE1353
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI Ksp632I KspI
MaeI MaeII MfeI MluI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 314 GGTACC G^GTACC
AccI 123 GTATAC GT^[AC][GT]AC
AccII 130 CGCG CG^CG
AccIII 290 TCCGGA T^CCGGA
AcyI 586 GACGCC G[GA]^CG[CT]C
AfaI 315 GTAC GT^AC
Aor51HI 227 AGCGCT AGC^GCT
Asp718I 314 GGTACC G^GTACC
AvaII 565 GGACC G^G[AT]CC
BglI 270 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 294 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 586 GACGCC G[GA]^CG[CT]C
BseAI 290 TCCGGA T^CCGGA
BsmAI 67 GTCTC GTCTC(1/5)
BsmI 367 GAATGC GAATGC(1/-1)
BspEI 290 TCCGGA T^CCGGA
BspHI 305 TCATGA T^CATGA
Bst1107I 123 GTATAC GTA^TAC
BstUI 130 CGCG CG^CG
BstXI 194 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 299 AGATCC [GA]^GATC[CT]
BstZ17I 123 GTATAC GTA^TAC
DsaI 188 CCGTGG C^C[GA][CT]GG
Eco47III 227 AGCGCT AGC^GCT
EcoT14I 608 CCAAGG C^C[AT][AT]GG
HgaI 586 GACGC GACGC(5/10)
Hin1I 586 GACGCC G[GA]^CG[CT]C
HinfI 460 GACTC G^A[ACGT]TC
HphI 494 TCACC GGTGA(8/7)
KpnI 314 GGTACC GGTAC^C
MaeIII 217 GTGAC ^GT[ACGT]AC
MamI 294 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 349 GAAGA GAAGA(8/7)
MflI 299 AGATCC [GA]^GATC[CT]
MroI 290 TCCGGA T^CCGGA
MvnI 130 CGCG CG^CG
PleI 460 GACTC GAGTC(4/5)
PstI 40 CTGCAG CTGCA^G
RcaI 305 TCATGA T^CATGA
RsaI 315 GTAC GT^AC
SacI 175 GAGCTC GAGCT^C
SfcI 40 CTGCAG C^T[GA][CT]AG
SmaI 146 CCCGGG CCC^GGG
StyI 608 CCAAGG C^C[AT][AT]GG
Tsp45I 217 GTGAC ^GT[GC]AC
XhoII 299 AGATCC [GA]^GATC[CT]
XmaCI 146 CCCGGG C^CCGGG
XmaI 146 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 300 GATCC GGATC(4/5)
309 GATCC
AspHI 175 GAGCTC G[AT]GC[AT]^C
473 GAGCAC
AvaI 146 CCCGGG C^[CT]CG[GA]G
318 CCCGAG
BalI 87 TGGCCA TGG^CCA
191 TGGCCA
BanII 3 GAGCCC G[GA]GC[CT]^C
175 GAGCTC
BpmI 506 CTCCAG CTGGAG(16/14)
259 CTGGAG
BsaWI 290 TCCGGA [AT]^CCGG[AT]
311 TCCGGT
BsgI 533 CTGCAC GTGCAG(16/14)
223 GTGCAG
BsiHKAI 175 GAGCTC G[AT]GC[AT]^C
473 GAGCAC
BsoBI 146 CCCGGG C^[CT]CG[GA]G
318 CCCGAG
BspMI 136 ACCTGC ACCTGC(4/8)
567 ACCTGC
BsrI 274 ACTGG ACTGG(1/-1)
360 CCAGT
BstF5I 499 CATCC GGATG(2/0)
293 GGATG
DpnII 300 GATC ^GATC
309 GATC
DraII 59 AGGCCCC [GA]G^G[ACGT]CC[CT]
516 AGGCCCC
EaeI 87 TGGCCA [CT]^GGCC[GA]
191 TGGCCA
EcoO109I 59 AGGCCCC [GA]G^G[ACGT]CC[CT]
516 AGGCCCC
FauI 52 CCCGC CCCGC(4/6)
614 GCGGG
FokI 499 CATCC GGATG(9/13)
293 GGATG
HaeII 227 AGCGCT [GA]GCGC^[CT]
550 AGCGCC
MboI 300 GATC ^GATC
309 GATC
MluNI 87 TGGCCA TGG^CCA
191 TGGCCA
MscI 87 TGGCCA TGG^CCA
191 TGGCCA
NdeII 300 GATC ^GATC
309 GATC
Sau3AI 300 GATC ^GATC
309 GATC
SfaNI 79 GATGC GCATC(5/9)
294 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PvuII Sau96I ScrFI
TseI TspRI