Restriction map for
pFN21AE6427
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BlpI Bpu1102I BsaAI
BsmBI BspDI BspHI BspLU11I BspMI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 507 GGCGTC G[GA]^CG[CT]C
Alw44I 1468 GTGCAC G^TGCAC
ApaLI 1468 GTGCAC G^TGCAC
AvaI 1201 CTCGGG C^[CT]CG[GA]G
BamHI 1352 GGATCC G^GATCC
BbsI 598 GAAGAC GAAGAC(2/6)
BciVI 1392 GGATAC GTATCC(6/5)
BpmI 1769 CTCCAG CTGGAG(16/14)
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaBI 1299 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 507 GGCGTC G[GA]^CG[CT]C
BsaI 63 GGTCTC GGTCTC(1/5)
BsgI 978 CTGCAC GTGCAG(16/14)
BsiWI 1232 CGTACG C^GTACG
BsoBI 1201 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1301 TGTACA T^GTACA
BsrDI 522 CATTGC GCAATG(2/0)
BsrGI 1301 TGTACA T^GTACA
BstXI 435 CCAGGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1528 CGGACCG CG^G[AT]CCG
DraII 213 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 590 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 213 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 1188 GGCGCT [GA]GCGC^[CT]
Hin1I 507 GGCGTC G[GA]^CG[CT]C
HindIII 1721 AAGCTT A^AGCTT
HphI 1143 GGTGA GGTGA(8/7)
MaeII 1061 ACGT A^CGT
MamI 1299 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1330 GCCGGC GCC^GGC
NgoMIV 1330 GCCGGC G^CCGGC
PpuMI 213 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 684 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1528 CGGACCG CG^G[AT]CCG
SphI 1326 GCATGC GCATG^C
SspBI 1301 TGTACA T^GTACA
SspI 661 AATATT AAT^ATT
XcmI 833 CCAAGATACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 72 CGCG CG^CG
1643 CGCG
AccIII 575 TCCGGA T^CCGGA
1295 TCCGGA
Asp700I 1146 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1734 GAATCTCTTC
AviII 23 TGCGCA TGC^GCA
89 TGCGCA
AvrII 132 CCTAGG C^CTAGG
309 CCTAGG
BcnI 51 CCGGG CC^[GC]GG
512 CCGGG
BlnI 132 CCTAGG C^CTAGG
309 CCTAGG
BsaWI 575 TCCGGA [AT]^CCGG[AT]
1295 TCCGGA
BseAI 575 TCCGGA T^CCGGA
1295 TCCGGA
BsmI 730 GCATTC GAATGC(1/-1)
791 GCATTC
BspEI 575 TCCGGA T^CCGGA
1295 TCCGGA
BsrBI 1653 CCGCTC CCGCTC(-3/-3)
3 GAGCGG
BssSI 1687 CACGAG CACGAG(-5/-1)
41 CTCGTG
BstUI 72 CGCG CG^CG
1643 CGCG
EcoNI 855 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
977 CCTGCACAAGG
FspI 23 TGCGCA TGC^GCA
89 TGCGCA
HgaI 508 GCGTC GACGC(5/10)
926 GCGTC
MroI 575 TCCGGA T^CCGGA
1295 TCCGGA
MvnI 72 CGCG CG^CG
1643 CGCG
NciI 51 CCGGG CC^[GC]GG
512 CCGGG
NcoI 1139 CCATGG C^CATGG
1458 CCATGG
NspI 1326 GCATGC [GA]CATG^[CT]
1858 ACATGC
PstI 1192 CTGCAG CTGCA^G
1321 CTGCAG
PvuII 1579 CAGCTG CAG^CTG
1753 CAGCTG
SapI 303 GAAGAGC GCTCTTC(1/4)
15 GCTCTTC
SfcI 1192 CTGCAG C^T[GA][CT]AG
1321 CTGCAG
TaqI 1130 TCGA T^CGA
1274 TCGA
TthHB8I 1130 TCGA T^CGA
1274 TCGA
XmnI 1146 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1734 GAATCTCTTC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanII
BbvI BfaI BmyI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EagI EarI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII