Restriction map for
pFN21AE4427
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 462 GACGTC GACGT^C
AcyI 462 GACGTC G[GA]^CG[CT]C
Aor51HI 80 AGCGCT AGC^GCT
Asp700I 948 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1150 GAGCAC G[AT]GC[AT]^C
AvaI 167 CCCGGG C^[CT]CG[GA]G
AvaII 40 GGACC G^G[AT]CC
BalI 603 TGGCCA TGG^CCA
BamHI 204 GGATCC G^GATCC
BanI 545 GGCACC G^G[CT][GA]CC
BbvCI 812 CCTCAGC CCTCAGC(-5/-2)
BclI 1041 TGATCA T^GATCA
BsaBI 1315 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 462 GACGTC G[GA]^CG[CT]C
BsaI 25 GAGACC GGTCTC(1/5)
BsaWI 1049 ACCGGA [AT]^CCGG[AT]
BsiEI 114 CGACCG CG[GA][CT]^CG
BsiHKAI 1150 GAGCAC G[AT]GC[AT]^C
BsoBI 167 CCCGGG C^[CT]CG[GA]G
Bsp1407I 125 TGTACA T^GTACA
BspMI 404 ACCTGC ACCTGC(4/8)
BsrDI 1091 GCAATG GCAATG(2/0)
BsrFI 149 GCCGGC [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
BstAPI 411 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 149 GCCGGC [GA]^CCGG[CT]
DraIII 1189 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 80 AGCGCT AGC^GCT
EcoT14I 719 CCTTGG C^C[AT][AT]GG
EcoT22I 700 ATGCAT ATGCA^T
FbaI 1041 TGATCA T^GATCA
HaeII 80 AGCGCT [GA]GCGC^[CT]
Hin1I 462 GACGTC G[GA]^CG[CT]C
HinfI 1294 GAGTC G^A[ACGT]TC
KspI 29 CCGCGG CCGC^GG
MaeIII 1009 GTGAC ^GT[ACGT]AC
MamI 1315 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 603 TGGCCA TGG^CCA
MscI 603 TGGCCA TGG^CCA
MspA1I 29 CCGCGG C[AC]G^C[GT]G
NaeI 149 GCCGGC GCC^GGC
NgoMIV 149 GCCGGC G^CCGGC
NsiI 700 ATGCAT ATGCA^T
PleI 1294 GAGTC GAGTC(4/5)
PpuMI 39 GGGACCT [GA]G^G[AT]CC[CT]
PstI 529 CTGCAG CTGCA^G
SacII 29 CCGCGG CCGC^GG
SfcI 529 CTGCAG C^T[GA][CT]AG
SmaI 167 CCCGGG CCC^GGG
SspBI 125 TGTACA T^GTACA
StyI 719 CCTTGG C^C[AT][AT]GG
Tsp45I 1009 GTGAC ^GT[GC]AC
XmaCI 167 CCCGGG C^CCGGG
XmaI 167 CCCGGG C^CCGGG
XmnI 948 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 30 CGCG CG^CG
50 CGCG
ApaI 617 GGGCCC GGGCC^C
808 GGGCCC
BbsI 388 GTCTTC GAAGAC(2/6)
556 GTCTTC
BfaI 106 CTAG C^TAG
1312 CTAG
BpmI 281 CTCCAG CTGGAG(16/14)
1078 CTCCAG
BpuAI 388 GTCTTC GAAGAC(2/6)
556 GTCTTC
BseRI 481 CTCCTC GAGGAG(10/8)
94 GAGGAG
BsmAI 25 GAGAC GTCTC(1/5)
279 GTCTC
BspHI 371 TCATGA T^CATGA
1044 TCATGA
BsrBI 97 GAGCGG CCGCTC(-3/-3)
358 GAGCGG
BstUI 30 CGCG CG^CG
50 CGCG
BstYI 204 GGATCC [GA]^GATC[CT]
728 AGATCC
CfoI 51 GCGC GCG^C
81 GCGC
DsaI 29 CCGCGG C^C[GA][CT]GG
200 CCACGG
EaeI 17 TGGCCG [CT]^GGCC[GA]
603 TGGCCA
HhaI 51 GCGC GCG^C
81 GCGC
HinP1I 51 GCGC G^CGC
81 GCGC
MaeI 106 CTAG C^TAG
1312 CTAG
MaeII 463 ACGT A^CGT
908 ACGT
MflI 204 GGATCC [GA]^GATC[CT]
728 AGATCC
MslI 693 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
924 CACTTATGTG
MvnI 30 CGCG CG^CG
50 CGCG
PflMI 626 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCACAAAGTGG
RcaI 371 TCATGA T^CATGA
1044 TCATGA
TaqI 113 TCGA T^CGA
1176 TCGA
Tsp509I 804 AATT ^AATT
1183 AATT
TthHB8I 113 TCGA T^CGA
1176 TCGA
Van91I 626 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCACAAAGTGG
XhoII 204 GGATCC [GA]^GATC[CT]
728 AGATCC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BcnI BmyI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI