Restriction map for
pFN21AE4411
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BspHI BspMI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MluI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SbfI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccII 1023 CGCG CG^CG
AccIII 506 TCCGGA T^CCGGA
AflIII 841 ACATGT A^C[GA][CT]GT
AspHI 719 GAGCAC G[AT]GC[AT]^C
AvaI 827 CCCGGG C^[CT]CG[GA]G
AvaII 704 GGTCC G^G[AT]CC
AvrII 573 CCTAGG C^CTAGG
BalI 407 TGGCCA TGG^CCA
BamHI 1200 GGATCC G^GATCC
BanI 774 GGTGCC G^G[CT][GA]CC
BanII 47 GGGCTC G[GA]GC[CT]^C
BbrPI 1228 CACGTG CAC^GTG
BlnI 573 CCTAGG C^CTAGG
BlpI 682 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 682 GCTAAGC GC^T[ACGT]AGC
BsaAI 1228 CACGTG [CT]AC^GT[GA]
BsaWI 506 TCCGGA [AT]^CCGG[AT]
BseAI 506 TCCGGA T^CCGGA
BsiHKAI 719 GAGCAC G[AT]GC[AT]^C
BsoBI 827 CCCGGG C^[CT]CG[GA]G
Bsp1407I 543 TGTACA T^GTACA
BspDI 751 ATCGAT AT^CGAT
BspEI 506 TCCGGA T^CCGGA
BspLU11I 841 ACATGT A^CATGT
BsrBI 111 CCGCTC CCGCTC(-3/-3)
BsrGI 543 TGTACA T^GTACA
BstUI 1023 CGCG CG^CG
BstXI 1113 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1200 GGATCC [GA]^GATC[CT]
CelII 682 GCTAAGC GC^T[ACGT]AGC
CfoI 762 GCGC GCG^C
ClaI 751 ATCGAT AT^CGAT
DraI 1129 TTTAAA TTT^AAA
EaeI 407 TGGCCA [CT]^GGCC[GA]
EarI 310 GAAGAG CTCTTC(1/4)
EcoRI 848 GAATTC G^AATTC
HhaI 762 GCGC GCG^C
HinP1I 762 GCGC G^CGC
HincII 1039 GTCGAC GT[CT]^[GA]AC
HindII 1039 GTCGAC GT[CT]^[GA]AC
Ksp632I 310 GAAGAG CTCTTC(1/4)
MaeII 1229 ACGT A^CGT
MfeI 466 CAATTG C^AATTG
MflI 1200 GGATCC [GA]^GATC[CT]
MluNI 407 TGGCCA TGG^CCA
MroI 506 TCCGGA T^CCGGA
MscI 407 TGGCCA TGG^CCA
MslI 10 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 58 CAGCTG C[AC]G^C[GT]G
MunI 466 CAATTG C^AATTG
MvnI 1023 CGCG CG^CG
NcoI 1143 CCATGG C^CATGG
PflMI 765 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1081 GAGTC GAGTC(4/5)
PmaCI 1228 CACGTG CAC^GTG
PmlI 1228 CACGTG CAC^GTG
PpuMI 703 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1221 CTGCAG CTGCA^G
PvuII 58 CAGCTG CAG^CTG
SalI 1039 GTCGAC G^TCGAC
SapI 310 GAAGAGC GCTCTTC(1/4)
SfaNI 139 GCATC GCATC(5/9)
SfiI 571 GGCCTAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 827 CCCGGG CCC^GGG
SspBI 543 TGTACA T^GTACA
Van91I 765 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1200 GGATCC [GA]^GATC[CT]
XmaCI 827 CCCGGG C^CCGGG
XmaI 827 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 562 GTAGAC GT^[AC][GT]AC
1039 GTCGAC
AcsI 848 GAATTC [GA]^AATT[CT]
877 GAATTT
AfaI 105 GTAC GT^AC
544 GTAC
ApoI 848 GAATTC [GA]^AATT[CT]
877 GAATTT
Asp700I 85 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
736 GAAAGTATTC
BbsI 34 GAAGAC GAAGAC(2/6)
85 GAAGAC
BglI 572 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
777 GCCTGGGCGGC
BpuAI 34 GAAGAC GAAGAC(2/6)
85 GAAGAC
BseRI 496 GAGGAG GAGGAG(10/8)
1153 GAGGAG
BsmAI 838 GAGAC GTCTC(1/5)
637 GTCTC
Cac8I 581 GCCCGC GC[ACGT]^[ACGT]GC
678 GCCAGC
DraII 703 AGGTCCT [GA]G^G[ACGT]CC[CT]
977 AGGGCCT
DsaI 9 CCACGG C^C[GA][CT]GG
1143 CCATGG
EcoNI 568 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
960 CCTCATACAGG
EcoO109I 703 AGGTCCT [GA]G^G[ACGT]CC[CT]
977 AGGGCCT
EcoT14I 573 CCTAGG C^C[AT][AT]GG
1143 CCATGG
FauI 27 CCCGC CCCGC(4/6)
582 CCCGC
NspI 171 ACATGC [GA]CATG^[CT]
841 ACATGT
RsaI 105 GTAC GT^AC
544 GTAC
SfcI 1215 CTGTAG C^T[GA][CT]AG
1221 CTGCAG
StyI 573 CCTAGG C^C[AT][AT]GG
1143 CCATGG
TfiI 999 GATTC G^A[AT]TC
1124 GATTC
XmnI 85 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
736 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BfaI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I