Restriction map for
pFN21AE3942
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI MamI MflI MluI MseI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 175 CGCG CG^CG
AccIII 738 TCCGGA T^CCGGA
AflIII 226 ACATGT A^C[GA][CT]GT
AocI 750 CCTGAGG CC^T[ACGT]AGG
AspHI 923 GAGCTC G[AT]GC[AT]^C
BbeI 860 GGCGCC GGCGC^C
BbvCI 641 CCTCAGC CCTCAGC(-5/-2)
BclI 440 TGATCA T^GATCA
BsaI 366 GGTCTC GGTCTC(1/5)
BseAI 738 TCCGGA T^CCGGA
BsiHKAI 923 GAGCTC G[AT]GC[AT]^C
BspEI 738 TCCGGA T^CCGGA
BspLU11I 226 ACATGT A^CATGT
BspMI 134 ACCTGC ACCTGC(4/8)
BsrFI 1184 GCCGGC [GA]^CCGG[CT]
BstEII 934 GGTGACC G^GT[ACGT]ACC
BstPI 934 GGTGACC G^GT[ACGT]ACC
BstUI 175 CGCG CG^CG
BstXI 419 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1184 GCCGGC [GA]^CCGG[CT]
DraII 961 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
EcoNI 1197 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 961 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 934 GGTGACC G^GT[ACGT]ACC
FbaI 440 TGATCA T^GATCA
HaeII 860 GGCGCC [GA]GCGC^[CT]
HgaI 353 GCGTC GACGC(5/10)
HincII 454 GTCAAC GT[CT]^[GA]AC
HindII 454 GTCAAC GT[CT]^[GA]AC
KasI 860 GGCGCC G^GCGCC
KspI 174 CCGCGG CCGC^GG
MfeI 1248 CAATTG C^AATTG
MroI 738 TCCGGA T^CCGGA
MunI 1248 CAATTG C^AATTG
MvnI 175 CGCG CG^CG
NaeI 1184 GCCGGC GCC^GGC
NarI 860 GGCGCC GG^CGCC
NcoI 590 CCATGG C^CATGG
NgoMIV 1184 GCCGGC G^CCGGC
NheI 699 GCTAGC G^CTAGC
NspI 226 ACATGT [GA]CATG^[CT]
PpuMI 961 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1041 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 923 GAGCTC GAGCT^C
SacII 174 CCGCGG CCGC^GG
SexAI 14 ACCTGGT A^CC[AT]GGT
SfoI 860 GGCGCC GGC^GCC
SpeI 154 ACTAGT A^CTAGT
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 352 GGCGTC G[GA]^CG[CT]C
860 GGCGCC
BanII 846 GGGCTC G[GA]GC[CT]^C
923 GAGCTC
BbsI 158 GTCTTC GAAGAC(2/6)
806 GTCTTC
BpuAI 158 GTCTTC GAAGAC(2/6)
806 GTCTTC
BsaHI 352 GGCGTC G[GA]^CG[CT]C
860 GGCGCC
BsaWI 218 TCCGGT [AT]^CCGG[AT]
738 TCCGGA
BsmAI 969 GAGAC GTCTC(1/5)
367 GTCTC
BsrBI 1255 CCGCTC CCGCTC(-3/-3)
1126 GAGCGG
BsrDI 191 CATTGC GCAATG(2/0)
414 CATTGC
EarI 980 CTCTTC CTCTTC(1/4)
1012 GAAGAG
Hin1I 352 GGCGTC G[GA]^CG[CT]C
860 GGCGCC
HinfI 179 GAGTC G^A[ACGT]TC
866 GAGTC
Ksp632I 980 CTCTTC CTCTTC(1/4)
1012 GAAGAG
MaeII 47 ACGT A^CGT
545 ACGT
MslI 414 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1032 CATCAGAGTG
PleI 179 GAGTC GAGTC(4/5)
866 GAGTC
PstI 883 CTGCAG CTGCA^G
1276 CTGCAG
PvuII 644 CAGCTG CAG^CTG
886 CAGCTG
SfcI 883 CTGCAG C^T[GA][CT]AG
1276 CTGCAG
TaqI 206 TCGA T^CGA
810 TCGA
Tsp509I 1106 AATT ^AATT
1249 AATT
TthHB8I 206 TCGA T^CGA
810 TCGA
XcmI 419 CCATGAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
575 CCAGCAGTGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AfaI AluI AvaII
BalI BanI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StuI StyI TseI
Tsp45I TspRI