Restriction map for
pFN21AE4556
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI ApaLI AscI Asp718I AspEI
AspI AvrII BbeI BbrPI BbsI
BbvCI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiWI BsmAI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 802 GAATTC [GA]^AATT[CT]
AocI 357 CCTGAGG CC^T[ACGT]AGG
Aor51HI 614 AGCGCT AGC^GCT
ApoI 802 GAATTC [GA]^AATT[CT]
AseI 1747 ATTAAT AT^TAAT
AsnI 1747 ATTAAT AT^TAAT
Asp700I 313 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 665 GAGCAC G[AT]GC[AT]^C
BanI 1869 GGCACC G^G[CT][GA]CC
BcgI 993 CGACTATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 892 GCCCGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1253 ACCGGA [AT]^CCGG[AT]
BsiEI 1320 CGATCG CG[GA][CT]^CG
BsiHKAI 665 GAGCAC G[AT]GC[AT]^C
BsmI 161 GCATTC GAATGC(1/-1)
Bsp1407I 914 TGTACA T^GTACA
BspLU11I 917 ACATGT A^CATGT
BsrBI 441 CCGCTC CCGCTC(-3/-3)
BsrDI 74 GCAATG GCAATG(2/0)
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BsrGI 914 TGTACA T^GTACA
BstAPI 1327 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1713 CCAGTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 357 CCTGAGG CC^T[ACGT]AGG
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
DraIII 1007 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1296 CTCTTC CTCTTC(1/4)
Eco47III 614 AGCGCT AGC^GCT
Eco81I 357 CCTGAGG CC^T[ACGT]AGG
EcoRI 802 GAATTC G^AATTC
HaeII 614 AGCGCT [GA]GCGC^[CT]
HincII 1794 GTTGAC GT[CT]^[GA]AC
HindII 1794 GTTGAC GT[CT]^[GA]AC
Ksp632I 1296 CTCTTC CTCTTC(1/4)
MspA1I 467 CAGCTG C[AC]G^C[GT]G
NspI 917 ACATGT [GA]CATG^[CT]
PshBI 1747 ATTAAT AT^TAAT
PvuI 1320 CGATCG CGAT^CG
PvuII 467 CAGCTG CAG^CTG
SbfI 696 CCTGCAGG CCTGCA^GG
Sse8387I 696 CCTGCAGG CCTGCA^GG
SspBI 914 TGTACA T^GTACA
SspI 314 AATATT AAT^ATT
TaqI 1323 TCGA T^CGA
TfiI 2011 GATTC G^A[AT]TC
TthHB8I 1323 TCGA T^CGA
XcmI 1844 CCATCACCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 313 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 62 CGCG CG^CG
64 CGCG
AflIII 917 ACATGT A^C[GA][CT]GT
1443 ACGTGT
AvaI 364 CCCGGG C^[CT]CG[GA]G
1781 CCCGGG
AviII 71 TGCGCA TGC^GCA
1559 TGCGCA
BamHI 1346 GGATCC G^GATCC
1682 GGATCC
BclI 791 TGATCA T^GATCA
1379 TGATCA
BfaI 418 CTAG C^TAG
820 CTAG
BpmI 1406 CTGGAG CTGGAG(16/14)
1861 CTGGAG
BseRI 444 CTCCTC GAGGAG(10/8)
514 CTCCTC
BsgI 380 GTGCAG GTGCAG(16/14)
547 GTGCAG
BsoBI 364 CCCGGG C^[CT]CG[GA]G
1781 CCCGGG
BspHI 264 TCATGA T^CATGA
333 TCATGA
BstUI 62 CGCG CG^CG
64 CGCG
BstYI 1346 GGATCC [GA]^GATC[CT]
1682 GGATCC
DsaI 713 CCACGG C^C[GA][CT]GG
894 CCGTGG
EcoT14I 1730 CCAAGG C^C[AT][AT]GG
1761 CCTTGG
FauI 182 CCCGC CCCGC(4/6)
1995 CCCGC
FbaI 791 TGATCA T^GATCA
1379 TGATCA
FspI 71 TGCGCA TGC^GCA
1559 TGCGCA
MaeI 418 CTAG C^TAG
820 CTAG
MflI 1346 GGATCC [GA]^GATC[CT]
1682 GGATCC
MvnI 62 CGCG CG^CG
64 CGCG
NdeI 1118 CATATG CA^TATG
1328 CATATG
RcaI 264 TCATGA T^CATGA
333 TCATGA
SmaI 364 CCCGGG CCC^GGG
1781 CCCGGG
StyI 1730 CCAAGG C^C[AT][AT]GG
1761 CCTTGG
XhoII 1346 GGATCC [GA]^GATC[CT]
1682 GGATCC
XmaCI 364 CCCGGG C^CCGGG
1781 CCCGGG
XmaI 364 CCCGGG C^CCGGG
1781 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BalI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I