Restriction map for
pFN21AE1404
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 43 GGTACC G^GTACC
AccI 983 GTCTAC GT^[AC][GT]AC
AcsI 744 AAATTC [GA]^AATT[CT]
AflIII 987 ACGTGT A^C[GA][CT]GT
Alw44I 110 GTGCAC G^TGCAC
AocI 216 CCTTAGG CC^T[ACGT]AGG
ApaI 396 GGGCCC GGGCC^C
ApaLI 110 GTGCAC G^TGCAC
ApoI 744 AAATTC [GA]^AATT[CT]
Asp718I 43 GGTACC G^GTACC
AspHI 110 GTGCAC G[AT]GC[AT]^C
AspI 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 51 TGGCCA TGG^CCA
BbsI 959 GTCTTC GAAGAC(2/6)
BpuAI 959 GTCTTC GAAGAC(2/6)
BsaAI 986 TACGTG [CT]AC^GT[GA]
BsaBI 934 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 604 GGTCTC GGTCTC(1/5)
BsaWI 966 ACCGGA [AT]^CCGG[AT]
BsiHKAI 110 GTGCAC G[AT]GC[AT]^C
BsrDI 689 CATTGC GCAATG(2/0)
BstAPI 693 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 407 CCAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 216 CCTTAGG CC^T[ACGT]AGG
DraI 538 TTTAAA TTT^AAA
EaeI 51 TGGCCA [CT]^GGCC[GA]
Eco81I 216 CCTTAGG CC^T[ACGT]AGG
EcoT22I 686 ATGCAT ATGCA^T
HapII 967 CCGG C^CGG
HindIII 793 AAGCTT A^AGCTT
HpaII 967 CCGG C^CGG
HphI 100 TCACC GGTGA(8/7)
KpnI 43 GGTACC GGTAC^C
MaeII 987 ACGT A^CGT
MamI 934 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 51 TGGCCA TGG^CCA
MscI 51 TGGCCA TGG^CCA
MspA1I 530 CAGCTG C[AC]G^C[GT]G
MspI 967 CCGG C^CGG
NlaIII 150 CATG CATG^
NsiI 686 ATGCAT ATGCA^T
PflFI 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 530 CAGCTG CAG^CTG
ScaI 56 AGTACT AGT^ACT
StuI 251 AGGCCT AGG^CCT
TaqI 557 TCGA T^CGA
TfiI 200 GAATC G^A[AT]TC
Tsp45I 1003 GTCAC ^GT[GC]AC
Tth111I 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 557 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 386 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
694 CAGTATCTG
BanI 43 GGTACC G^G[CT][GA]CC
189 GGTGCC
BanII 396 GGGCCC G[GA]GC[CT]^C
456 GGGCTC
BfaI 289 CTAG C^TAG
304 CTAG
BsaJI 764 CCCTGG C^C[ACGT][ACGT]GG
891 CCTGGG
BsmAI 605 GTCTC GTCTC(1/5)
613 GTCTC
EarI 22 CTCTTC CTCTTC(1/4)
617 CTCTTC
Ksp632I 22 CTCTTC CTCTTC(1/4)
617 CTCTTC
MaeI 289 CTAG C^TAG
304 CTAG
MslI 113 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
238 CATATTGATG
PleI 3 GAGTC GAGTC(4/5)
388 GAGTC
PstI 274 CTGCAG CTGCA^G
424 CTGCAG
SfaNI 520 GATGC GCATC(5/9)
944 GCATC
SfcI 274 CTGCAG C^T[GA][CT]AG
424 CTGCAG
SmlI 570 CTTGAG C^T[CT][GA]AG
856 CTTGAG
TspRI 48 CAGTG CA[GC]TG[ACGT][ACGT]^
277 CAGTG
XbaI 288 TCTAGA T^CTAGA
303 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BpmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I XhoII