Restriction map for
pFN21AE2386
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# NO CUT ENZYMES ( 203 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BbvI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DdeI DraI DraII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI Fnu4HI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII ItaI KasI KpnI
Ksp632I KspI MaeII MamI MboII
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI TseI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 318 GCGG CCGC(-3/-1)
AcsI 45 GAATTT [GA]^AATT[CT]
ApoI 45 GAATTT [GA]^AATT[CT]
BalI 98 TGGCCA TGG^CCA
BanI 19 GGCACC G^G[CT][GA]CC
BcgI 41 GCAAGAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 31 CTAG C^TAG
BmyI 18 GGGCAC G[AGT]GC[ACT]^C
BsaI 304 GAGACC GGTCTC(1/5)
Bsp1286I 18 GGGCAC G[AGT]GC[ACT]^C
BsrI 258 ACTGG ACTGG(1/-1)
BstXI 89 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 100 GCCAGC GC[ACGT]^[ACGT]GC
DpnII 186 GATC ^GATC
DraIII 68 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 232 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 357 CCACGG C^C[GA][CT]GG
EaeI 98 TGGCCA [CT]^GGCC[GA]
FauI 318 GCGGG CCCGC(4/6)
HaeIII 99 GGCC GG^CC
HgaI 7 GACGC GACGC(5/10)
MaeI 31 CTAG C^TAG
MboI 186 GATC ^GATC
MluNI 98 TGGCCA TGG^CCA
MscI 98 TGGCCA TGG^CCA
MseI 194 TTAA T^TAA
MslI 309 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 186 GATC ^GATC
NlaIV 19 GGCACC GG[ACGT]^[ACGT]CC
Sau3AI 186 GATC ^GATC
Tru9I 194 TTAA T^TAA
XcmI 248 CCATTGTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsaJI 15 CCTGGG C^C[ACGT][ACGT]GG
357 CCACGG
BsmAI 217 GAGAC GTCTC(1/5)
304 GAGAC
BsmFI 140 GGGAC GGGAC(10/14)
293 GGGAC
BspMI 281 ACCTGC ACCTGC(4/8)
93 GCAGGT
BstF5I 342 CATCC GGATG(2/0)
261 GGATG
FokI 342 CATCC GGATG(9/13)
261 GGATG
MaeIII 66 GTCAC ^GT[ACGT]AC
253 GTGAC
MnlI 266 GAGG CCTC(7/6)
394 GAGG
NlaIII 60 CATG CATG^
105 CATG
PstI 283 CTGCAG CTGCA^G
365 CTGCAG
SfcI 283 CTGCAG C^T[GA][CT]AG
365 CTGCAG
Tsp45I 66 GTCAC ^GT[GC]AC
253 GTGAC
Tsp509I 46 AATT ^AATT
327 AATT
TspRI 257 CACTG CA[GC]TG[ACGT][ACGT]^
363 CACTG
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AluI BssKI BstNI EcoRII HphI
MvaI ScrFI TaqI TthHB8I