Restriction map for
pFN21AE4555
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BcgI BfrI BlnI BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Cfr10I CpoI DraI DraIII
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 625 GGTACC G^GTACC
AfaI 626 GTAC GT^AC
AocI 938 CCTCAGG CC^T[ACGT]AGG
AseI 213 ATTAAT AT^TAAT
AsnI 213 ATTAAT AT^TAAT
Asp718I 625 GGTACC G^GTACC
AspI 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1662 TGGCCA TGG^CCA
BbvCI 916 GCTGAGG CCTCAGC(-5/-2)
BciVI 1038 GTATCC GTATCC(6/5)
BclI 1902 TGATCA T^GATCA
BglI 10 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 704 AGATCT A^GATCT
BlpI 43 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC
BsaBI 212 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmAI 551 GAGAC GTCTC(1/5)
BsmI 1064 GAATGC GAATGC(1/-1)
BspDI 1045 ATCGAT AT^CGAT
BssSI 1751 CTCGTG CACGAG(-5/-1)
BstEII 561 GGTCACC G^GT[ACGT]ACC
BstPI 561 GGTCACC G^GT[ACGT]ACC
BstXI 376 CCATTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 938 CCTCAGG CC^T[ACGT]AGG
CelII 43 GCTGAGC GC^T[ACGT]AGC
CfoI 1310 GCGC GCG^C
ClaI 1045 ATCGAT AT^CGAT
DraII 1424 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 553 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 938 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1424 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 561 GGTCACC G^GT[ACGT]ACC
FbaI 1902 TGATCA T^GATCA
HapII 1656 CCGG C^CGG
HhaI 1310 GCGC GCG^C
HinP1I 1310 GCGC G^CGC
HincII 805 GTTGAC GT[CT]^[GA]AC
HindII 805 GTTGAC GT[CT]^[GA]AC
HpaII 1656 CCGG C^CGG
KpnI 625 GGTACC GGTAC^C
MamI 212 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1662 TGGCCA TGG^CCA
MscI 1662 TGGCCA TGG^CCA
MspI 1656 CCGG C^CGG
NdeI 440 CATATG CA^TATG
NotI 4 GCGGCCGC GC^GGCCGC
NspI 1462 GCATGC [GA]CATG^[CT]
PaeR7I 1397 CTCGAG C^TCGAG
PflFI 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1424 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 213 ATTAAT AT^TAAT
PstI 1598 CTGCAG CTGCA^G
RsaI 626 GTAC GT^AC
SmaI 1655 CCCGGG CCC^GGG
SmlI 1397 CTCGAG C^T[CT][GA]AG
SphI 1462 GCATGC GCATG^C
SspI 57 AATATT AAT^ATT
StuI 935 AGGCCT AGG^CCT
Tth111I 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1397 CTCGAG C^TCGAG
XmaCI 1655 CCCGGG C^CCGGG
XmaI 1655 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AvaI 1397 CTCGAG C^[CT]CG[GA]G
1655 CCCGGG
BcnI 1655 CCCGG CC^[GC]GG
1656 CCGGG
BfaI 518 CTAG C^TAG
607 CTAG
BsiYI 1206 CCCAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1771 CCCCAGTTTGG
BslI 1206 CCCAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1771 CCCCAGTTTGG
BsoBI 1397 CTCGAG C^[CT]CG[GA]G
1655 CCCGGG
BspMI 1883 ACCTGC ACCTGC(4/8)
1548 GCAGGT
DsaI 797 CCATGG C^C[GA][CT]GG
1847 CCATGG
EarI 1978 CTCTTC CTCTTC(1/4)
889 GAAGAG
FauI 1422 GCGGG CCCGC(4/6)
1473 GCGGG
Ksp632I 1978 CTCTTC CTCTTC(1/4)
889 GAAGAG
MaeI 518 CTAG C^TAG
607 CTAG
MaeII 849 ACGT A^CGT
1907 ACGT
MslI 1029 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1261 CACAAAGATG
NciI 1655 CCCGG CC^[GC]GG
1656 CCGGG
NcoI 797 CCATGG C^CATGG
1847 CCATGG
PvuII 41 CAGCTG CAG^CTG
289 CAGCTG
SfaNI 265 GATGC GCATC(5/9)
1603 GCATC
XcmI 1074 CCAACGATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1723 CCAAAAGCTCTGTGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanI BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII