Restriction map for
pFN21AE4481
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AclI AflII Alw44I
AocI Aor51HI ApaLI AscI Asp700I
AspI AviII AvrII BalI BamHI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsgI BsiEI BsmI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI MamI
MluI MluNI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PmeI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI
SgrAI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 378 GGTACC G^GTACC
AccIII 181 TCCGGA T^CCGGA
AcyI 1290 GACGCC G[GA]^CG[CT]C
AgeI 375 ACCGGT A^CCGGT
AhdI 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 830 GGATC GGATC(4/5)
ApaI 315 GGGCCC GGGCC^C
AseI 1059 ATTAAT AT^TAAT
AsnI 1059 ATTAAT AT^TAAT
Asp718I 378 GGTACC G^GTACC
AspEI 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1302 GGACC G^G[AT]CC
BbrPI 60 CACGTG CAC^GTG
BbsI 654 GTCTTC GAAGAC(2/6)
BpuAI 654 GTCTTC GAAGAC(2/6)
BsaHI 1290 GACGCC G[GA]^CG[CT]C
BsaI 1020 GAGACC GGTCTC(1/5)
BseAI 181 TCCGGA T^CCGGA
BsiWI 438 CGTACG C^GTACG
BspDI 899 ATCGAT AT^CGAT
BspEI 181 TCCGGA T^CCGGA
BspHI 1307 TCATGA T^CATGA
BsrDI 289 GCAATG GCAATG(2/0)
BsrFI 375 ACCGGT [GA]^CCGG[CT]
BstAPI 264 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 240 TTCGAA TT^CGAA
BstYI 830 GGATCT [GA]^GATC[CT]
CfoI 1318 GCGC GCG^C
Cfr10I 375 ACCGGT [GA]^CCGG[CT]
ClaI 899 ATCGAT AT^CGAT
DraII 314 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 23 CGGCCA [CT]^GGCC[GA]
Eam1105I 10 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 314 AGGGCCC [GA]G^G[ACGT]CC[CT]
HhaI 1318 GCGC GCG^C
Hin1I 1290 GACGCC G[GA]^CG[CT]C
HinP1I 1318 GCGC G^CGC
KpnI 378 GGTACC GGTAC^C
KspI 851 CCGCGG CCGC^GG
MaeIII 1000 GTTAC ^GT[ACGT]AC
MflI 830 GGATCT [GA]^GATC[CT]
MroI 181 TCCGGA T^CCGGA
MslI 1221 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 851 CCGCGG C[AC]G^C[GT]G
NspV 240 TTCGAA TT^CGAA
PinAI 375 ACCGGT A^CCGGT
PmaCI 60 CACGTG CAC^GTG
PmlI 60 CACGTG CAC^GTG
PshBI 1059 ATTAAT AT^TAAT
PstI 628 CTGCAG CTGCA^G
RcaI 1307 TCATGA T^CATGA
SacII 851 CCGCGG CCGC^GG
ScaI 1485 AGTACT AGT^ACT
SfcI 628 CTGCAG C^T[GA][CT]AG
SfuI 240 TTCGAA TT^CGAA
SmaI 1136 CCCGGG CCC^GGG
SnaBI 544 TACGTA TAC^GTA
SpeI 1482 ACTAGT A^CTAGT
SphI 307 GCATGC GCATG^C
XhoII 830 GGATCT [GA]^GATC[CT]
XmaCI 1136 CCCGGG C^CCGGG
XmaI 1136 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 852 CGCG CG^CG
1317 CGCG
AvaI 586 CCCGAG C^[CT]CG[GA]G
1136 CCCGGG
BanI 187 GGCACC G^G[CT][GA]CC
378 GGTACC
BcnI 1136 CCCGG CC^[GC]GG
1137 CCGGG
BpmI 1094 CTCCAG CTGGAG(16/14)
116 CTGGAG
BsaAI 60 CACGTG [CT]AC^GT[GA]
544 TACGTA
BsaWI 181 TCCGGA [AT]^CCGG[AT]
375 ACCGGT
BseRI 85 GAGGAG GAGGAG(10/8)
88 GAGGAG
BsmBI 91 GAGACG CGTCTC(1/5)
1288 GAGACG
BsoBI 586 CCCGAG C^[CT]CG[GA]G
1136 CCCGGG
Bsp1407I 845 TGTACA T^GTACA
1439 TGTACA
BsrGI 845 TGTACA T^GTACA
1439 TGTACA
BstUI 852 CGCG CG^CG
1317 CGCG
BstXI 613 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
914 CCAAAAAATTGG
DdeI 869 CTAAG C^T[ACGT]AG
1198 CTGAG
DsaI 851 CCGCGG C^C[GA][CT]GG
1220 CCACGG
EcoRV 277 GATATC GAT^ATC
1039 GATATC
FauI 65 GCGGG CCCGC(4/6)
853 GCGGG
HaeIII 24 GGCC GG^CC
316 GGCC
HphI 1224 GGTGA GGTGA(8/7)
583 TCACC
MfeI 416 CAATTG C^AATTG
1443 CAATTG
MunI 416 CAATTG C^AATTG
1443 CAATTG
MvnI 852 CGCG CG^CG
1317 CGCG
NciI 1136 CCCGG CC^[GC]GG
1137 CCGGG
NspI 307 GCATGC [GA]CATG^[CT]
883 ACATGC
PleI 199 GACTC GAGTC(4/5)
16 GAGTC
SspBI 845 TGTACA T^GTACA
1439 TGTACA
TfiI 211 GATTC G^A[AT]TC
1465 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI BanII BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EarI EcoRII Fnu4HI FokI
HapII HgaI HinfI HpaII ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I