Restriction map for
pFN21AE5208
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstUI BstYI
BstZ17I Bsu36I ClaI DraI DraII
DraIII Eam1105I EarI Eco47III Eco81I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HinfI
HpaI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 299 ACGTGT A^C[GA][CT]GT
AlwI 201 GGATC GGATC(4/5)
AspI 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 305 CCCGGG C^[CT]CG[GA]G
AvaII 360 GGTCC G^G[AT]CC
BcgI 380 GCAGCAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 415 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC
BsaI 547 GGTCTC GGTCTC(1/5)
BsiEI 390 CGGCCG CG[GA][CT]^CG
BsmAI 548 GTCTC GTCTC(1/5)
BsmI 643 GAATGC GAATGC(1/-1)
BsoBI 305 CCCGGG C^[CT]CG[GA]G
BsrFI 392 GCCGGT [GA]^CCGG[CT]
BstBI 640 TTCGAA TT^CGAA
BstEII 30 GGTGACC G^GT[ACGT]ACC
BstPI 30 GGTGACC G^GT[ACGT]ACC
BstXI 473 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 415 GCTCAGC GC^T[ACGT]AGC
Cfr10I 392 GCCGGT [GA]^CCGG[CT]
CpoI 359 CGGTCCG CG^G[AT]CCG
DrdI 9 GACGGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 390 CGGCCG C^GGCCG
EclXI 390 CGGCCG C^GGCCG
Eco52I 390 CGGCCG C^GGCCG
EcoNI 444 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 30 GGTGACC G^GT[ACGT]ACC
EcoT14I 282 CCTTGG C^C[AT][AT]GG
HincII 175 GTTGAC GT[CT]^[GA]AC
HindII 175 GTTGAC GT[CT]^[GA]AC
MspA1I 489 CCGCTG C[AC]G^C[GT]G
NspV 640 TTCGAA TT^CGAA
PflFI 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 438 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 359 CGGTCCG CG^G[AT]CCG
SfuI 640 TTCGAA TT^CGAA
SmaI 305 CCCGGG CCC^GGG
StyI 282 CCTTGG C^C[AT][AT]GG
TaqI 641 TCGA T^CGA
Tth111I 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 641 TCGA T^CGA
Van91I 438 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 648 CCAGGCCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 305 CCCGGG C^CCGGG
XmaI 305 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 274 GGCGCC G[GA]^CG[CT]C
661 GGCGCC
AfaI 318 GTAC GT^AC
396 GTAC
AlwNI 439 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
486 CAGCCGCTG
BanII 222 GGGCTC G[GA]GC[CT]^C
413 GGGCTC
BbeI 274 GGCGCC GGCGC^C
661 GGCGCC
BpmI 121 CTGGAG CTGGAG(16/14)
160 CTGGAG
BsaHI 274 GGCGCC G[GA]^CG[CT]C
661 GGCGCC
Cfr13I 360 GGTCC G^G[ACGT]CC
459 GGCCC
DdeI 104 CTGAG C^T[ACGT]AG
416 CTCAG
DpnII 202 GATC ^GATC
324 GATC
DsaI 26 CCACGG C^C[GA][CT]GG
479 CCGTGG
EaeI 390 CGGCCG [CT]^GGCC[GA]
446 TGGCCG
Hin1I 274 GGCGCC G[GA]^CG[CT]C
661 GGCGCC
KasI 274 GGCGCC G^GCGCC
661 GGCGCC
MaeII 257 ACGT A^CGT
299 ACGT
MboI 202 GATC ^GATC
324 GATC
MslI 474 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CACCGTCATG
NarI 274 GGCGCC GG^CGCC
661 GGCGCC
NdeII 202 GATC ^GATC
324 GATC
NlaIII 153 CATG CATG^
537 CATG
RsaI 318 GTAC GT^AC
396 GTAC
Sau3AI 202 GATC ^GATC
324 GATC
Sau96I 360 GGTCC G^G[ACGT]CC
459 GGCCC
SfaNI 211 GATGC GCATC(5/9)
251 GCATC
SfcI 189 CTACAG C^T[GA][CT]AG
233 CTGTAG
SfoI 274 GGCGCC GGC^GCC
661 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BanI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MnlI MspI MvaI
MwoI NciI NlaIV ScrFI TseI
Tsp45I TspRI