Restriction map for
pFN21AE5501
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 190 GACGTC GACGT^C
AccI 868 GTAGAC GT^[AC][GT]AC
AccIII 790 TCCGGA T^CCGGA
AhdI 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 162 GGGCCC GGGCC^C
AspEI 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 939 GTATCC GTATCC(6/5)
BglI 91 GCCGATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 472 TACGTG [CT]AC^GT[GA]
BsaI 259 GAGACC GGTCTC(1/5)
BseAI 790 TCCGGA T^CCGGA
BsgI 277 GTGCAG GTGCAG(16/14)
BsmBI 1204 GAGACG CGTCTC(1/5)
BspEI 790 TCCGGA T^CCGGA
BssHII 331 GCGCGC G^CGCGC
BstEII 549 GGTCACC G^GT[ACGT]ACC
BstPI 549 GGTCACC G^GT[ACGT]ACC
Eam1105I 274 GACGTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 549 GGTCACC G^GT[ACGT]ACC
KspI 825 CCGCGG CCGC^GG
MroI 790 TCCGGA T^CCGGA
MseI 350 TTAA T^TAA
MslI 468 CATTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 885 CCATGG C^CATGG
NspI 701 GCATGT [GA]CATG^[CT]
PflFI 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 366 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 128 AGGACCT [GA]G^G[AT]CC[CT]
PstI 142 CTGCAG CTGCA^G
PvuII 145 CAGCTG CAG^CTG
SacI 1138 GAGCTC GAGCT^C
SacII 825 CCGCGG CCGC^GG
ScaI 1121 AGTACT AGT^ACT
SmlI 590 CTCAAG C^T[CT][GA]AG
StuI 899 AGGCCT AGG^CCT
Tru9I 350 TTAA T^TAA
Tth111I 714 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 366 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 839 CCAATTCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 332 CGCG CG^CG
826 CGCG
AcyI 190 GACGTC G[GA]^CG[CT]C
461 GGCGTC
AfaI 106 GTAC GT^AC
1122 GTAC
AvaI 326 CCCGGG C^[CT]CG[GA]G
539 CCCGGG
BanII 162 GGGCCC G[GA]GC[CT]^C
1138 GAGCTC
BglII 227 AGATCT A^GATCT
380 AGATCT
BsaHI 190 GACGTC G[GA]^CG[CT]C
461 GGCGTC
BsaWI 320 TCCGGT [AT]^CCGG[AT]
790 TCCGGA
BsoBI 326 CCCGGG C^[CT]CG[GA]G
539 CCCGGG
BspMI 140 ACCTGC ACCTGC(4/8)
398 ACCTGC
BstAPI 66 GCATCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
279 GCAGTCGCTGC
BstUI 332 CGCG CG^CG
826 CGCG
BstXI 508 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
555 CCAGGCCAGTGG
HaeII 1154 GGCGCT [GA]GCGC^[CT]
1215 GGCGCT
Hin1I 190 GACGTC G[GA]^CG[CT]C
461 GGCGTC
MboII 582 GAAGA GAAGA(8/7)
644 TCTTC
MspA1I 145 CAGCTG C[AC]G^C[GT]G
825 CCGCGG
MvnI 332 CGCG CG^CG
826 CGCG
PleI 714 GACTC GAGTC(4/5)
238 GAGTC
RsaI 106 GTAC GT^AC
1122 GTAC
SmaI 326 CCCGGG CCC^GGG
539 CCCGGG
Tsp509I 841 AATT ^AATT
1068 AATT
XmaCI 326 CCCGGG C^CCGGG
539 CCCGGG
XmaI 326 CCCGGG C^CCGGG
539 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AciI AluI AlwI AlwNI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII