Restriction map for
pFN21AE6350
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbvCI BcgI BciVI BclI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BspDI BspEI BspHI
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tsp45I
Tth111I XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AflII 634 CTTAAG C^TTAAG
AseI 895 ATTAAT AT^TAAT
AsnI 895 ATTAAT AT^TAAT
BanI 114 GGTGCC G^G[CT][GA]CC
BbrPI 244 CACGTG CAC^GTG
BbsI 461 GTCTTC GAAGAC(2/6)
BfaI 715 CTAG C^TAG
BfrI 634 CTTAAG C^TTAAG
BmyI 115 GTGCCC G[AGT]GC[ACT]^C
BpuAI 461 GTCTTC GAAGAC(2/6)
BsaAI 244 CACGTG [CT]AC^GT[GA]
BsmI 643 GAATGC GAATGC(1/-1)
Bsp1286I 115 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 742 TGTACA T^GTACA
BspLU11I 415 ACATGT A^CATGT
BsrDI 1003 CATTGC GCAATG(2/0)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BsrGI 742 TGTACA T^GTACA
BsrI 205 CCAGT ACTGG(1/-1)
BstAPI 261 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 300 CCAATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 25 GCGC GCG^C
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
DdeI 950 CTGAG C^T[ACGT]AG
DraII 729 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 729 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 998 GAATTC G^AATTC
EcoRV 527 GATATC GAT^ATC
EcoT14I 15 CCTTGG C^C[AT][AT]GG
HhaI 25 GCGC GCG^C
HinP1I 25 GCGC G^CGC
MaeI 715 CTAG C^TAG
MaeIII 256 GTAAC ^GT[ACGT]AC
NaeI 55 GCCGGC GCC^GGC
NgoMIV 55 GCCGGC G^CCGGC
NspI 415 ACATGT [GA]CATG^[CT]
PflMI 910 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 237 GAGTC GAGTC(4/5)
PmaCI 244 CACGTG CAC^GTG
PmlI 244 CACGTG CAC^GTG
PpuMI 729 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 895 ATTAAT AT^TAAT
PstI 966 CTGCAG CTGCA^G
SfcI 966 CTGCAG C^T[GA][CT]AG
SmaI 47 CCCGGG CCC^GGG
SmlI 634 CTTAAG C^T[CT][GA]AG
SspBI 742 TGTACA T^GTACA
SspI 559 AATATT AAT^ATT
StuI 5 AGGCCT AGG^CCT
StyI 15 CCTTGG C^C[AT][AT]GG
TfiI 172 GATTC G^A[AT]TC
Van91I 910 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 714 TCTAGA T^CTAGA
XcmI 292 CCATCATTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 26 CGCG CG^CG
652 CGCG
AfaI 743 GTAC GT^AC
875 GTAC
AlwI 190 GGATC GGATC(4/5)
976 GGATC
AvaI 47 CCCGGG C^[CT]CG[GA]G
71 CTCGGG
AvaII 44 GGACC G^G[AT]CC
730 GGACC
BcnI 47 CCCGG CC^[GC]GG
48 CCGGG
BseRI 85 CTCCTC GAGGAG(10/8)
391 GAGGAG
BsgI 347 CTGCAC GTGCAG(16/14)
839 GTGCAG
BsoBI 47 CCCGGG C^[CT]CG[GA]G
71 CTCGGG
BstUI 26 CGCG CG^CG
652 CGCG
Cac8I 35 GCAGGC GC[ACGT]^[ACGT]GC
55 GCCGGC
FauI 30 GCGGG CCCGC(4/6)
111 GCGGG
HapII 48 CCGG C^CGG
56 CCGG
HinfI 172 GATTC G^A[ACGT]TC
237 GAGTC
HpaII 48 CCGG C^CGG
56 CCGG
MslI 296 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
751 CATAACTGTG
MspI 48 CCGG C^CGG
56 CCGG
MvnI 26 CGCG CG^CG
652 CGCG
NciI 47 CCCGG CC^[GC]GG
48 CCGGG
RsaI 743 GTAC GT^AC
875 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcsI AflIII AluI ApoI
BbvI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MaeII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I