##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       ItaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TseI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII          21  CGCG                 CG^CG
AfaI          368  GTAC                 GT^AC
AluI          305  AGCT                 AG^CT
Asp700I        99  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          577  GAAGAC               GAAGAC(2/6)
BpuAI         577  GAAGAC               GAAGAC(2/6)
BsmFI         263  GGGAC                GGGAC(10/14)
BspHI         537  TCATGA               T^CATGA
BsrI          455  ACTGG                ACTGG(1/-1)
BstBI         401  TTCGAA               TT^CGAA
BstF5I        240  CATCC                GGATG(2/0)
BstUI          21  CGCG                 CG^CG
DdeI          165  CTTAG                C^T[ACGT]AG
DraI          489  TTTAAA               TTT^AAA
EarI          297  GAAGAG               CTCTTC(1/4)
FokI          240  CATCC                GGATG(9/13)
HphI          522  GGTGA                GGTGA(8/7)
Ksp632I       297  GAAGAG               CTCTTC(1/4)
MaeIII         40  GTCAC                ^GT[ACGT]AC
MvnI           21  CGCG                 CG^CG
NspV          401  TTCGAA               TT^CGAA
PflMI         432  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           30  GACTC                GAGTC(4/5)
RcaI          537  TCATGA               T^CATGA
RsaI          368  GTAC                 GT^AC
SfuI          401  TTCGAA               TT^CGAA
SspI          382  AATATT               AAT^ATT
TaqI          402  TCGA                 T^CGA
Tsp45I         40  GTCAC                ^GT[GC]AC
TspRI         371  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       402  TCGA                 T^CGA
Van91I        432  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI           99  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI           22  GCGG                 CCGC(-3/-1)
              517  GCGG                
AcsI          353  AAATTC               [GA]^AATT[CT]
              607  GAATTT              
ApoI          353  AAATTC               [GA]^AATT[CT]
              607  GAATTT              
BstYI         415  AGATCC               [GA]^GATC[CT]
              441  GGATCT              
MflI          415  AGATCC               [GA]^GATC[CT]
              441  GGATCT              
MslI          205  CATAACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              535  CATCATGATG          
NlaIV         178  GGCTCC               GG[ACGT]^[ACGT]CC
              263  GGGACC              
SfaNI         541  GATGC                GCATC(5/9)
              239  GCATC               
TfiI          399  GATTC                G^A[AT]TC
              424  GAATC               
XhoII         415  AGATCC               [GA]^GATC[CT]
              441  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AlwI        AvaII       BpmI        BsaJI       BsiYI       
BslI        BssKI       BstNI       Cfr13I      DpnII       
EcoRII      HinfI       MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      Sau3AI      
Sau96I      ScrFI       Tru9I       Tsp509I