Restriction map for
pFN21AB6323
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 295 CCGC CCGC(-3/-1)
AflIII 545 ACATGT A^C[GA][CT]GT
AlwI 755 GGATC GGATC(4/5)
AlwNI 380 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 461 CCTCAGG CC^T[ACGT]AGG
ApaI 687 GGGCCC GGGCC^C
BanI 46 GGTGCC G^G[CT][GA]CC
BfaI 554 CTAG C^TAG
BpmI 348 CTCCAG CTGGAG(16/14)
BsaWI 746 ACCGGA [AT]^CCGG[AT]
BseRI 487 GAGGAG GAGGAG(10/8)
BsmAI 66 GTCTC GTCTC(1/5)
BsmI 99 GCATTC GAATGC(1/-1)
BspLU11I 545 ACATGT A^CATGT
BsrBI 295 CCGCTC CCGCTC(-3/-3)
BsrDI 193 CATTGC GCAATG(2/0)
Bsu36I 461 CCTCAGG CC^T[ACGT]AGG
CfoI 498 GCGC GCG^C
DraIII 143 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 461 CCTCAGG CC^T[ACGT]AGG
EcoT14I 731 CCAAGG C^C[AT][AT]GG
FauI 294 CCCGC CCCGC(4/6)
HapII 747 CCGG C^CGG
HhaI 498 GCGC GCG^C
HinP1I 498 GCGC G^CGC
HpaII 747 CCGG C^CGG
MaeI 554 CTAG C^TAG
MaeIII 574 GTGAC ^GT[ACGT]AC
MslI 729 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 185 CAGCTG C[AC]G^C[GT]G
MspI 747 CCGG C^CGG
NspI 545 ACATGT [GA]CATG^[CT]
PvuII 185 CAGCTG CAG^CTG
SexAI 8 ACCTGGT A^CC[AT]GGT
StyI 731 CCAAGG C^C[AT][AT]GG
TaqI 479 TCGA T^CGA
TfiI 634 GATTC G^A[AT]TC
Tsp45I 574 GTGAC ^GT[GC]AC
TthHB8I 479 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 126 GTAC GT^AC
726 GTAC
Alw44I 39 GTGCAC G^TGCAC
163 GTGCAC
ApaLI 39 GTGCAC G^TGCAC
163 GTGCAC
BanII 598 GGGCTC G[GA]GC[CT]^C
687 GGGCCC
Bsp1407I 125 TGTACA T^GTACA
725 TGTACA
BspMI 451 ACCTGC ACCTGC(4/8)
114 GCAGGT
BsrGI 125 TGTACA T^GTACA
725 TGTACA
BstF5I 60 CATCC GGATG(2/0)
136 GGATG
DpnII 393 GATC ^GATC
756 GATC
FokI 60 CATCC GGATG(9/13)
136 GGATG
HphI 573 GGTGA GGTGA(8/7)
631 GGTGA
MboI 393 GATC ^GATC
756 GATC
NdeII 393 GATC ^GATC
756 GATC
PleI 346 GACTC GAGTC(4/5)
507 GAGTC
RsaI 126 GTAC GT^AC
726 GTAC
Sau3AI 393 GATC ^GATC
756 GATC
SfaNI 321 GCATC GCATC(5/9)
476 GCATC
SspBI 125 TGTACA T^GTACA
725 TGTACA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AspHI AvaII BbsI BbvI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HinfI
ItaI MboII MnlI MvaI MwoI
NlaIII NlaIV PpuMI Sau96I ScrFI
TseI TspRI