Restriction map for
pFN21AE6345
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BstBI
BstUI BstYI Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 322 GGTACC G^GTACC
AcyI 853 GGCGCC G[GA]^CG[CT]C
Alw44I 460 GTGCAC G^TGCAC
AlwI 784 GGATC GGATC(4/5)
ApaI 828 GGGCCC GGGCC^C
ApaLI 460 GTGCAC G^TGCAC
Asp718I 322 GGTACC G^GTACC
BalI 977 TGGCCA TGG^CCA
BbeI 853 GGCGCC GGCGC^C
BlpI 1012 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1012 GCTCAGC GC^T[ACGT]AGC
BsaHI 853 GGCGCC G[GA]^CG[CT]C
BsaWI 355 ACCGGA [AT]^CCGG[AT]
BseRI 151 GAGGAG GAGGAG(10/8)
BsmI 80 GAATGC GAATGC(1/-1)
BspHI 602 TCATGA T^CATGA
BssSI 1044 CTCGTG CACGAG(-5/-1)
Bst1107I 766 GTATAC GTA^TAC
BstEII 415 GGTCACC G^GT[ACGT]ACC
BstPI 415 GGTCACC G^GT[ACGT]ACC
BstZ17I 766 GTATAC GTA^TAC
CelII 1012 GCTCAGC GC^T[ACGT]AGC
DdeI 1013 CTCAG C^T[ACGT]AG
DraIII 70 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 646 CTCTTC CTCTTC(1/4)
EcoNI 422 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 415 GGTCACC G^GT[ACGT]ACC
Hin1I 853 GGCGCC G[GA]^CG[CT]C
HincII 310 GTCAAC GT[CT]^[GA]AC
HindII 310 GTCAAC GT[CT]^[GA]AC
HinfI 7 GACTC G^A[ACGT]TC
KasI 853 GGCGCC G^GCGCC
KpnI 322 GGTACC GGTAC^C
Ksp632I 646 CTCTTC CTCTTC(1/4)
MluNI 977 TGGCCA TGG^CCA
MscI 977 TGGCCA TGG^CCA
NarI 853 GGCGCC GG^CGCC
PleI 7 GACTC GAGTC(4/5)
PpuMI 138 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 224 CAGCTG CAG^CTG
RcaI 602 TCATGA T^CATGA
SapI 645 GCTCTTC GCTCTTC(1/4)
SexAI 35 ACCTGGT A^CC[AT]GGT
SfiI 654 GGCCATCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 853 GGCGCC GGC^GCC
SmaI 725 CCCGGG CCC^GGG
TaqI 338 TCGA T^CGA
TthHB8I 338 TCGA T^CGA
XmaCI 725 CCCGGG C^CCGGG
XmaI 725 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 272 ACGTGT A^C[GA][CT]GT
556 ACGTGT
AvaI 106 CTCGGG C^[CT]CG[GA]G
725 CCCGGG
BbrPI 70 CACGTG CAC^GTG
712 CACGTG
BbsI 159 GAAGAC GAAGAC(2/6)
439 GAAGAC
BpuAI 159 GAAGAC GAAGAC(2/6)
439 GAAGAC
BsaAI 70 CACGTG [CT]AC^GT[GA]
712 CACGTG
BsoBI 106 CTCGGG C^[CT]CG[GA]G
725 CCCGGG
BsrDI 863 CATTGC GCAATG(2/0)
695 GCAATG
BstAPI 69 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
915 GCAGTGGCTGC
BstXI 252 CCACACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
380 CCAGCAGCATGG
HaeII 853 GGCGCC [GA]GCGC^[CT]
1016 AGCGCC
PmaCI 70 CACGTG CAC^GTG
712 CACGTG
PmlI 70 CACGTG CAC^GTG
712 CACGTG
PstI 88 CTGCAG CTGCA^G
913 CTGCAG
SfaNI 708 GATGC GCATC(5/9)
578 GCATC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfcI TseI Tsp45I
TspRI Van91I