Restriction map for
pFN21AE5203
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FokI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MamI MluI MluNI
MroI MscI MslI MspI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 139 CGCG CG^CG
AcsI 595 AAATTC [GA]^AATT[CT]
ApoI 595 AAATTC [GA]^AATT[CT]
Asp700I 627 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 68 GAGCAC G[AT]GC[AT]^C
BbsI 511 GAAGAC GAAGAC(2/6)
BglII 399 AGATCT A^GATCT
BpmI 172 CTGGAG CTGGAG(16/14)
BpuAI 511 GAAGAC GAAGAC(2/6)
BsaJI 80 CCGAGG C^C[ACGT][ACGT]GG
BsgI 339 GTGCAG GTGCAG(16/14)
BsiHKAI 68 GAGCAC G[AT]GC[AT]^C
BsmAI 27 GTCTC GTCTC(1/5)
BsmFI 332 GGGAC GGGAC(10/14)
BstUI 139 CGCG CG^CG
BstYI 399 AGATCT [GA]^GATC[CT]
DdeI 29 CTCAG C^T[ACGT]AG
DrdI 46 GACTCCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3 GAAGAG CTCTTC(1/4)
EcoT22I 20 ATGCAT ATGCA^T
Ksp632I 3 GAAGAG CTCTTC(1/4)
MaeIII 424 GTGAC ^GT[ACGT]AC
MfeI 650 CAATTG C^AATTG
MflI 399 AGATCT [GA]^GATC[CT]
MunI 650 CAATTG C^AATTG
MvnI 139 CGCG CG^CG
NlaIV 332 GGGACC GG[ACGT]^[ACGT]CC
NsiI 20 ATGCAT ATGCA^T
PleI 46 GACTC GAGTC(4/5)
PpuMI 332 GGGACCT [GA]G^G[AT]CC[CT]
PstI 151 CTGCAG CTGCA^G
PvuII 148 CAGCTG CAG^CTG
ScaI 176 AGTACT AGT^ACT
SmlI 349 CTCAAG C^T[CT][GA]AG
Tsp45I 424 GTGAC ^GT[GC]AC
XhoII 399 AGATCT [GA]^GATC[CT]
XmnI 627 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 177 GTAC GT^AC
324 GTAC
BanII 126 GAGCCC G[GA]GC[CT]^C
346 GGGCTC
BssKI 171 CCTGG ^CC[ACGT]GG
233 CCAGG
BstNI 171 CCTGG CC^[AT]GG
233 CCAGG
DpnII 12 GATC ^GATC
400 GATC
DraII 94 AGGGCCT [GA]G^G[ACGT]CC[CT]
332 GGGACCT
EcoNI 86 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
468 CCTTCTACAGG
EcoO109I 94 AGGGCCT [GA]G^G[ACGT]CC[CT]
332 GGGACCT
EcoRII 171 CCTGG ^CC[AT]GG
233 CCAGG
HphI 182 TCACC GGTGA(8/7)
503 TCACC
MboI 12 GATC ^GATC
400 GATC
MseI 266 TTAA T^TAA
391 TTAA
MspA1I 73 CAGCGG C[AC]G^C[GT]G
148 CAGCTG
MvaI 171 CCTGG CC^[AT]GG
233 CCAGG
NdeII 12 GATC ^GATC
400 GATC
RsaI 177 GTAC GT^AC
324 GTAC
Sau3AI 12 GATC ^GATC
400 GATC
ScrFI 171 CCTGG CC^[ACGT]GG
233 CCAGG
SfcI 151 CTGCAG C^T[GA][CT]AG
472 CTACAG
StuI 83 AGGCCT AGG^CCT
222 AGGCCT
TfiI 375 GATTC G^A[AT]TC
464 GAATC
Tru9I 266 TTAA T^TAA
391 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsiYI BslI Bsp1286I Cac8I CfoI
Cfr13I FauI Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MboII MnlI
MwoI NlaIII Sau96I TseI Tsp509I
TspRI