Restriction map for
pFN21AE6441
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BseRI BsiWI
BsmI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstBI BstYI BstZ17I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MflI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 640 GTCTAC GT^[AC][GT]AC
AccIII 1310 TCCGGA T^CCGGA
AcsI 245 GAATTC [GA]^AATT[CT]
AlwI 1285 GATCC GGATC(4/5)
AlwNI 743 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 748 CCTGAGG CC^T[ACGT]AGG
Aor51HI 71 AGCGCT AGC^GCT
ApoI 245 GAATTC [GA]^AATT[CT]
Asp700I 1005 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 293 CCCGAG C^[CT]CG[GA]G
BlpI 1252 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1252 GCTCAGC GC^T[ACGT]AGC
BsaWI 1310 TCCGGA [AT]^CCGG[AT]
BseAI 1310 TCCGGA T^CCGGA
BsiEI 769 CGGTCG CG[GA][CT]^CG
BsmBI 814 CGTCTC CGTCTC(1/5)
BsmFI 564 GTCCC GGGAC(10/14)
BsoBI 293 CCCGAG C^[CT]CG[GA]G
BspEI 1310 TCCGGA T^CCGGA
BspHI 701 TCATGA T^CATGA
BspLU11I 1142 ACATGT A^CATGT
BsrDI 411 CATTGC GCAATG(2/0)
BsrFI 692 ACCGGC [GA]^CCGG[CT]
BssSI 849 CACGAG CACGAG(-5/-1)
BstAPI 1362 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 186 GGTGACC G^GT[ACGT]ACC
BstPI 186 GGTGACC G^GT[ACGT]ACC
Bsu36I 748 CCTGAGG CC^T[ACGT]AGG
CelII 1252 GCTCAGC GC^T[ACGT]AGC
Cfr10I 692 ACCGGC [GA]^CCGG[CT]
DpnII 1285 GATC ^GATC
DraI 664 TTTAAA TTT^AAA
DrdI 1081 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 697 CTCTTC CTCTTC(1/4)
Eco47III 71 AGCGCT AGC^GCT
Eco81I 748 CCTGAGG CC^T[ACGT]AGG
EcoO65I 186 GGTGACC G^GT[ACGT]ACC
EcoRI 245 GAATTC G^AATTC
HincII 261 GTTGAC GT[CT]^[GA]AC
HindII 261 GTTGAC GT[CT]^[GA]AC
Ksp632I 697 CTCTTC CTCTTC(1/4)
MboI 1285 GATC ^GATC
MluI 811 ACGCGT A^CGCGT
MroI 1310 TCCGGA T^CCGGA
MseI 665 TTAA T^TAA
NdeII 1285 GATC ^GATC
PflFI 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 752 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1255 CAGCTG CAG^CTG
RcaI 701 TCATGA T^CATGA
SacI 226 GAGCTC GAGCT^C
SapI 696 GCTCTTC GCTCTTC(1/4)
Sau3AI 1285 GATC ^GATC
SphI 716 GCATGC GCATG^C
Tru9I 665 TTAA T^TAA
Tsp509I 246 AATT ^AATT
TspRI 1110 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1005 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 156 CGCG CG^CG
812 CGCG
AcyI 77 GGCGCC G[GA]^CG[CT]C
557 GGCGCC
ApaI 289 GGGCCC GGGCC^C
734 GGGCCC
AspHI 226 GAGCTC G[AT]GC[AT]^C
1369 GTGCTC
BalI 167 TGGCCA TGG^CCA
530 TGGCCA
BbeI 77 GGCGCC GGCGC^C
557 GGCGCC
BbsI 601 GAAGAC GAAGAC(2/6)
868 GAAGAC
BcnI 81 CCGGG CC^[GC]GG
95 CCCGG
BfaI 407 CTAG C^TAG
1069 CTAG
BpuAI 601 GAAGAC GAAGAC(2/6)
868 GAAGAC
BsaHI 77 GGCGCC G[GA]^CG[CT]C
557 GGCGCC
BsiHKAI 226 GAGCTC G[AT]GC[AT]^C
1369 GTGCTC
BsmAI 670 GAGAC GTCTC(1/5)
815 GTCTC
BstUI 156 CGCG CG^CG
812 CGCG
HgaI 810 GACGC GACGC(5/10)
813 GCGTC
Hin1I 77 GGCGCC G[GA]^CG[CT]C
557 GGCGCC
KasI 77 GGCGCC G^GCGCC
557 GGCGCC
MaeI 407 CTAG C^TAG
1069 CTAG
MaeII 125 ACGT A^CGT
1102 ACGT
MluNI 167 TGGCCA TGG^CCA
530 TGGCCA
MscI 167 TGGCCA TGG^CCA
530 TGGCCA
MslI 469 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1128 CATGAAGGTG
MspA1I 46 CCGCTG C[AC]G^C[GT]G
1255 CAGCTG
MvnI 156 CGCG CG^CG
812 CGCG
NarI 77 GGCGCC GG^CGCC
557 GGCGCC
NciI 81 CCGGG CC^[GC]GG
95 CCCGG
PstI 685 CTGCAG CTGCA^G
1258 CTGCAG
SfcI 685 CTGCAG C^T[GA][CT]AG
1258 CTGCAG
SfoI 77 GGCGCC GGC^GCC
557 GGCGCC
XcmI 485 CCATCATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1127 CCATGAAGGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaII
BanI BanII BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
NspI PleI RsaI Sau96I ScrFI
SfaNI TseI Tsp45I