Restriction map for
pFN21AB6931
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# NO CUT ENZYMES ( 165 enzymes) #
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Acc65I AclI AcsI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
FauI FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MscI
MspA1I MunI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 337 GACGTC GACGT^C
AccI 259 GTCTAC GT^[AC][GT]AC
AccII 453 CGCG CG^CG
AccIII 296 TCCGGA T^CCGGA
AciI 78 CCGC CCGC(-3/-1)
BanII 151 GAGCTC G[GA]GC[CT]^C
BsaI 445 GAGACC GGTCTC(1/5)
BseAI 296 TCCGGA T^CCGGA
BspEI 296 TCCGGA T^CCGGA
BsrDI 241 GCAATG GCAATG(2/0)
BsrFI 193 GCCGGT [GA]^CCGG[CT]
BsrI 302 ACTGG ACTGG(1/-1)
BstF5I 294 CATCC GGATG(2/0)
BstUI 453 CGCG CG^CG
CfoI 452 GCGC GCG^C
Cfr10I 193 GCCGGT [GA]^CCGG[CT]
DraI 133 TTTAAA TTT^AAA
EarI 588 GAAGAG CTCTTC(1/4)
EcoNI 227 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 502 GATATC GAT^ATC
EcoT14I 395 CCAAGG C^C[AT][AT]GG
EcoT22I 479 ATGCAT ATGCA^T
FokI 294 CATCC GGATG(9/13)
HgaI 91 GACGC GACGC(5/10)
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
HphI 269 TCACC GGTGA(8/7)
Ksp632I 588 GAAGAG CTCTTC(1/4)
MaeII 338 ACGT A^CGT
MroI 296 TCCGGA T^CCGGA
MslI 465 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 453 CGCG CG^CG
NsiI 479 ATGCAT ATGCA^T
PshAI 63 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 151 GAGCTC GAGCT^C
SfaNI 248 GCATC GCATC(5/9)
StyI 395 CCAAGG C^C[AT][AT]GG
XcmI 461 CCAACATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
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AcyI 91 GACGCC G[GA]^CG[CT]C
337 GACGTC
Asp700I 202 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
279 GAAGTCCTTC
AspHI 151 GAGCTC G[AT]GC[AT]^C
322 GAGCAC
AvaI 48 CTCGGG C^[CT]CG[GA]G
355 CTCGGG
AvaII 143 GGACC G^G[AT]CC
579 GGACC
BbsI 6 GAAGAC GAAGAC(2/6)
60 GAAGAC
BmyI 151 GAGCTC G[AGT]GC[ACT]^C
322 GAGCAC
BpuAI 6 GAAGAC GAAGAC(2/6)
60 GAAGAC
BsaHI 91 GACGCC G[GA]^CG[CT]C
337 GACGTC
BsaWI 296 TCCGGA [AT]^CCGG[AT]
576 ACCGGA
BseRI 319 GAGGAG GAGGAG(10/8)
591 GAGGAG
BsiHKAI 151 GAGCTC G[AT]GC[AT]^C
322 GAGCAC
BsiYI 227 CCTACTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
395 CCAAGGAACGG
BslI 227 CCTACTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
395 CCAAGGAACGG
BsmAI 384 GAGAC GTCTC(1/5)
445 GAGAC
BsmFI 44 GGGAC GGGAC(10/14)
185 GGGAC
BsoBI 48 CTCGGG C^[CT]CG[GA]G
355 CTCGGG
Bsp1286I 151 GAGCTC G[AGT]GC[ACT]^C
322 GAGCAC
BssKI 546 CCAGG ^CC[ACGT]GG
558 CCAGG
BstNI 546 CCAGG CC^[AT]GG
558 CCAGG
Cac8I 324 GCACGC GC[ACGT]^[ACGT]GC
414 GCTGGC
DpnII 39 GATC ^GATC
216 GATC
EcoRII 546 CCAGG ^CC[AT]GG
558 CCAGG
Hin1I 91 GACGCC G[GA]^CG[CT]C
337 GACGTC
MboI 39 GATC ^GATC
216 GATC
MseI 134 TTAA T^TAA
170 TTAA
MvaI 546 CCAGG CC^[AT]GG
558 CCAGG
NdeII 39 GATC ^GATC
216 GATC
NlaIII 252 CATG CATG^
441 CATG
PleI 46 GACTC GAGTC(4/5)
563 GAGTC
PstI 175 CTGCAG CTGCA^G
332 CTGCAG
Sau3AI 39 GATC ^GATC
216 GATC
ScrFI 546 CCAGG CC^[ACGT]GG
558 CCAGG
TfiI 306 GATTC G^A[AT]TC
431 GAATC
Tru9I 134 TTAA T^TAA
170 TTAA
XmnI 202 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
279 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BsaJI Cfr13I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MboII MnlI MspI Sau96I SfcI
TaqI TseI Tsp509I TthHB8I