Restriction map for
pFN21AE3624
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AspI AvrII BbeI BcgI BciVI
BclI BlnI BsaBI BsiEI BsiWI
BsmI BspDI BspLU11I BssHII Bst1107I
BstAPI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoNI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII ScaI
SfiI SfoI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 586 GTCGAC GT^[AC][GT]AC
AcyI 1768 GACGCC G[GA]^CG[CT]C
AflII 1581 CTTAAG C^TTAAG
AocI 1351 CCTGAGG CC^T[ACGT]AGG
Aor51HI 26 AGCGCT AGC^GCT
ApaI 736 GGGCCC GGGCC^C
AseI 1362 ATTAAT AT^TAAT
AsnI 1362 ATTAAT AT^TAAT
Asp700I 1506 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1757 TGCGCA TGC^GCA
BamHI 1526 GGATCC G^GATCC
BbrPI 822 CACGTG CAC^GTG
BfaI 1210 CTAG C^TAG
BfrI 1581 CTTAAG C^TTAAG
BglI 109 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1487 AGATCT A^GATCT
BlpI 1336 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1336 GCTAAGC GC^T[ACGT]AGC
BsaAI 822 CACGTG [CT]AC^GT[GA]
BsaHI 1768 GACGCC G[GA]^CG[CT]C
Bsp1407I 1273 TGTACA T^GTACA
BspHI 830 TCATGA T^CATGA
BsrFI 156 GCCGGT [GA]^CCGG[CT]
BsrGI 1273 TGTACA T^GTACA
BstBI 806 TTCGAA TT^CGAA
BstEII 399 GGTGACC G^GT[ACGT]ACC
BstPI 399 GGTGACC G^GT[ACGT]ACC
Bsu36I 1351 CCTGAGG CC^T[ACGT]AGG
CelII 1336 GCTAAGC GC^T[ACGT]AGC
Cfr10I 156 GCCGGT [GA]^CCGG[CT]
DraIII 702 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1723 GACTCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 26 AGCGCT AGC^GCT
Eco81I 1351 CCTGAGG CC^T[ACGT]AGG
EcoO65I 399 GGTGACC G^GT[ACGT]ACC
EcoRI 774 GAATTC G^AATTC
FspI 1757 TGCGCA TGC^GCA
HaeII 26 AGCGCT [GA]GCGC^[CT]
Hin1I 1768 GACGCC G[GA]^CG[CT]C
HincII 586 GTCGAC GT[CT]^[GA]AC
HindII 586 GTCGAC GT[CT]^[GA]AC
MaeI 1210 CTAG C^TAG
MslI 1734 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1658 CCATGG C^CATGG
NdeI 972 CATATG CA^TATG
NspV 806 TTCGAA TT^CGAA
PmaCI 822 CACGTG CAC^GTG
PmlI 822 CACGTG CAC^GTG
PshAI 1136 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1362 ATTAAT AT^TAAT
PvuII 567 CAGCTG CAG^CTG
RcaI 830 TCATGA T^CATGA
SalI 586 GTCGAC G^TCGAC
SbfI 543 CCTGCAGG CCTGCA^GG
SexAI 581 ACCTGGT A^CC[AT]GGT
SfuI 806 TTCGAA TT^CGAA
SmlI 1581 CTTAAG C^T[CT][GA]AG
Sse8387I 543 CCTGCAGG CCTGCA^GG
SspBI 1273 TGTACA T^GTACA
XbaI 1209 TCTAGA T^CTAGA
XcmI 789 CCAGGGTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1506 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 524 CGCG CG^CG
1043 CGCG
AccIII 458 TCCGGA T^CCGGA
1648 TCCGGA
AcsI 774 GAATTC [GA]^AATT[CT]
1588 GAATTT
AflIII 821 ACACGT A^C[GA][CT]GT
1270 ACGTGT
ApoI 774 GAATTC [GA]^AATT[CT]
1588 GAATTT
BalI 1665 TGGCCA TGG^CCA
1793 TGGCCA
BanI 82 GGTGCC G^G[CT][GA]CC
630 GGTGCC
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
363 CCTCAGC
BseAI 458 TCCGGA T^CCGGA
1648 TCCGGA
BseRI 12 CTCCTC GAGGAG(10/8)
204 CTCCTC
BsgI 537 CTGCAC GTGCAG(16/14)
910 CTGCAC
BsmBI 712 GAGACG CGTCTC(1/5)
764 GAGACG
BspEI 458 TCCGGA T^CCGGA
1648 TCCGGA
BsrBI 744 CCGCTC CCGCTC(-3/-3)
298 GAGCGG
BsrDI 1332 CATTGC GCAATG(2/0)
923 GCAATG
BssSI 653 CACGAG CACGAG(-5/-1)
940 CTCGTG
BstUI 524 CGCG CG^CG
1043 CGCG
BstXI 488 CCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
989 CCAATGATGTGG
DsaI 915 CCACGG C^C[GA][CT]GG
1658 CCATGG
EcoT14I 813 CCTTGG C^C[AT][AT]GG
1658 CCATGG
MaeII 823 ACGT A^CGT
1270 ACGT
MluNI 1665 TGGCCA TGG^CCA
1793 TGGCCA
MroI 458 TCCGGA T^CCGGA
1648 TCCGGA
MscI 1665 TGGCCA TGG^CCA
1793 TGGCCA
MseI 1363 TTAA T^TAA
1582 TTAA
MvnI 524 CGCG CG^CG
1043 CGCG
NspI 899 ACATGC [GA]CATG^[CT]
1423 ACATGC
PflMI 411 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
789 CCAGGGTCTGG
PleI 1723 GACTC GAGTC(4/5)
656 GAGTC
PpuMI 905 GGGACCT [GA]G^G[AT]CC[CT]
1316 GGGACCT
PstI 544 CTGCAG CTGCA^G
844 CTGCAG
SacI 532 GAGCTC GAGCT^C
1260 GAGCTC
SapI 1380 GCTCTTC GCTCTTC(1/4)
1779 GCTCTTC
SfcI 544 CTGCAG C^T[GA][CT]AG
844 CTGCAG
StyI 813 CCTTGG C^C[AT][AT]GG
1658 CCATGG
Tru9I 1363 TTAA T^TAA
1582 TTAA
Van91I 411 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
789 CCAGGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI