Restriction map for
pFN21AE3660
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AfaI 990 GTAC GT^AC
AlwI 182 GGATC GGATC(4/5)
AseI 655 ATTAAT AT^TAAT
AsnI 655 ATTAAT AT^TAAT
AvaI 1112 CCCGGG C^[CT]CG[GA]G
BbsI 1313 GTCTTC GAAGAC(2/6)
BbvCI 1095 CCTCAGC CCTCAGC(-5/-2)
BclI 764 TGATCA T^GATCA
BpuAI 1313 GTCTTC GAAGAC(2/6)
BsaWI 179 ACCGGA [AT]^CCGG[AT]
BsmBI 808 GAGACG CGTCTC(1/5)
BsoBI 1112 CCCGGG C^[CT]CG[GA]G
Bsp1407I 989 TGTACA T^GTACA
BspMI 1262 GCAGGT ACCTGC(4/8)
BsrDI 541 GCAATG GCAATG(2/0)
BsrGI 989 TGTACA T^GTACA
BstEII 720 GGTCACC G^GT[ACGT]ACC
BstF5I 198 GGATG GGATG(2/0)
BstPI 720 GGTCACC G^GT[ACGT]ACC
BstYI 182 GGATCT [GA]^GATC[CT]
DraI 757 TTTAAA TTT^AAA
EcoNI 78 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 720 GGTCACC G^GT[ACGT]ACC
FbaI 764 TGATCA T^GATCA
FokI 198 GGATG GGATG(9/13)
HaeII 844 AGCGCC [GA]GCGC^[CT]
HincII 115 GTTGAC GT[CT]^[GA]AC
HindII 115 GTTGAC GT[CT]^[GA]AC
MaeII 1289 ACGT A^CGT
MflI 182 GGATCT [GA]^GATC[CT]
NheI 204 GCTAGC G^CTAGC
NspI 1278 GCATGT [GA]CATG^[CT]
PflMI 189 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 230 GAGTC GAGTC(4/5)
PshBI 655 ATTAAT AT^TAAT
RsaI 990 GTAC GT^AC
SexAI 83 ACCAGGT A^CC[AT]GGT
SmaI 1112 CCCGGG CCC^GGG
SspBI 989 TGTACA T^GTACA
Van91I 189 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 182 GGATCT [GA]^GATC[CT]
XmaCI 1112 CCCGGG C^CCGGG
XmaI 1112 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 27 CGCG CG^CG
1268 CGCG
AlwNI 592 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
1080 CAGCAGCTG
BalI 686 TGGCCA TGG^CCA
1206 TGGCCA
BcnI 1112 CCCGG CC^[GC]GG
1113 CCGGG
BfaI 205 CTAG C^TAG
275 CTAG
BsgI 90 GTGCAG GTGCAG(16/14)
679 GTGCAG
BsmAI 386 GAGAC GTCTC(1/5)
808 GAGAC
BsmI 567 GCATTC GAATGC(1/-1)
817 GCATTC
BstUI 27 CGCG CG^CG
1268 CGCG
CfoI 845 GCGC GCG^C
1232 GCGC
DpnII 183 GATC ^GATC
765 GATC
DraII 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
1091 AGGGCCT
DraIII 1210 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1212 CACTGTGTG
EcoO109I 305 AGGCCCT [GA]G^G[ACGT]CC[CT]
1091 AGGGCCT
FauI 576 GCGGG CCCGC(4/6)
785 GCGGG
HgaI 25 GACGC GACGC(5/10)
810 GACGC
HhaI 845 GCGC GCG^C
1232 GCGC
HinP1I 845 GCGC G^CGC
1232 GCGC
HphI 151 GGTGA GGTGA(8/7)
722 TCACC
MaeI 205 CTAG C^TAG
275 CTAG
MboI 183 GATC ^GATC
765 GATC
MboII 239 TCTTC GAAGA(8/7)
1314 TCTTC
MluNI 686 TGGCCA TGG^CCA
1206 TGGCCA
MscI 686 TGGCCA TGG^CCA
1206 TGGCCA
MvnI 27 CGCG CG^CG
1268 CGCG
NciI 1112 CCCGG CC^[GC]GG
1113 CCGGG
NdeII 183 GATC ^GATC
765 GATC
NlaIV 246 GGAGCC GG[ACGT]^[ACGT]CC
493 GGGACC
SacI 424 GAGCTC GAGCT^C
439 GAGCTC
Sau3AI 183 GATC ^GATC
765 GATC
Tsp509I 630 AATT ^AATT
654 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BglI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstAPI BstNI Cac8I
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeIII MnlI MseI MspA1I
MspI MvaI MwoI NlaIII PstI
PvuII Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
TspRI XcmI