Restriction map for
pFN21AE6324
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI SalI SbfI
ScaI SexAI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Aor51HI 575 AGCGCT AGC^GCT
BalI 530 TGGCCA TGG^CCA
BamHI 963 GGATCC G^GATCC
BbeI 95 GGCGCC GGCGC^C
BbsI 415 GTCTTC GAAGAC(2/6)
BbvCI 957 GCTGAGG CCTCAGC(-5/-2)
BglI 217 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 751 CTGGAG CTGGAG(16/14)
BpuAI 415 GTCTTC GAAGAC(2/6)
BseRI 664 CTCCTC GAGGAG(10/8)
BsiEI 24 CGGCCG CG[GA][CT]^CG
BsmAI 613 GTCTC GTCTC(1/5)
Bsp1407I 314 TGTACA T^GTACA
BsrDI 66 GCAATG GCAATG(2/0)
BsrFI 26 GCCGGT [GA]^CCGG[CT]
BsrGI 314 TGTACA T^GTACA
BssHII 78 GCGCGC G^CGCGC
BstAPI 66 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 963 GGATCC [GA]^GATC[CT]
Cfr10I 26 GCCGGT [GA]^CCGG[CT]
CpoI 28 CGGTCCG CG^G[AT]CCG
DraIII 184 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 24 CGGCCG C^GGCCG
EclXI 24 CGGCCG C^GGCCG
Eco47III 575 AGCGCT AGC^GCT
Eco52I 24 CGGCCG C^GGCCG
HgaI 239 GCGTC GACGC(5/10)
HincII 879 GTTGAC GT[CT]^[GA]AC
HindII 879 GTTGAC GT[CT]^[GA]AC
KasI 95 GGCGCC G^GCGCC
KspI 38 CCGCGG CCGC^GG
MaeII 1082 ACGT A^CGT
MflI 963 GGATCC [GA]^GATC[CT]
MluNI 530 TGGCCA TGG^CCA
MscI 530 TGGCCA TGG^CCA
NarI 95 GGCGCC GG^CGCC
NcoI 533 CCATGG C^CATGG
NdeI 1230 CATATG CA^TATG
PleI 56 GACTC GAGTC(4/5)
PvuII 302 CAGCTG CAG^CTG
RsrII 28 CGGTCCG CG^G[AT]CCG
SacI 1177 GAGCTC GAGCT^C
SacII 38 CCGCGG CCGC^GG
SfiI 216 GGCCTACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 95 GGCGCC GGC^GCC
SspBI 314 TGTACA T^GTACA
StuI 215 AGGCCT AGG^CCT
TfiI 707 GAATC G^A[AT]TC
Tsp45I 278 GTGAC ^GT[GC]AC
XhoII 963 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 250 GGTACC G^GTACC
444 GGTACC
AcyI 95 GGCGCC G[GA]^CG[CT]C
238 GGCGTC
AlwI 964 GATCC GGATC(4/5)
963 GGATC
AlwNI 611 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGTTTCTG
ApaI 689 GGGCCC GGGCC^C
1266 GGGCCC
Asp718I 250 GGTACC G^GTACC
444 GGTACC
AvaII 29 GGTCC G^G[AT]CC
286 GGACC
BsaHI 95 GGCGCC G[GA]^CG[CT]C
238 GGCGTC
BsaWI 31 TCCGGT [AT]^CCGG[AT]
283 ACCGGA
BsgI 1127 CTGCAC GTGCAG(16/14)
1225 CTGCAC
BsmFI 54 GGGAC GGGAC(10/14)
774 GTCCC
DraII 688 GGGGCCC [GA]G^G[ACGT]CC[CT]
689 GGGCCCT
EcoO109I 688 GGGGCCC [GA]G^G[ACGT]CC[CT]
689 GGGCCCT
EcoT14I 533 CCATGG C^C[AT][AT]GG
789 CCTTGG
Hin1I 95 GGCGCC G[GA]^CG[CT]C
238 GGCGTC
HinfI 56 GACTC G^A[ACGT]TC
707 GAATC
KpnI 250 GGTACC GGTAC^C
444 GGTACC
MaeIII 278 GTGAC ^GT[ACGT]AC
651 GTTAC
StyI 533 CCATGG C^C[AT][AT]GG
789 CCTTGG
TaqI 858 TCGA T^CGA
1175 TCGA
TthHB8I 858 TCGA T^CGA
1175 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BanI BanII BbvI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI TspRI