Restriction map for
pFN21AE6323
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 888 TCCGGA T^CCGGA
AlwNI 1199 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 651 GAGCAC G[AT]GC[AT]^C
AspI 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 575 TGGCCA TGG^CCA
BbvCI 568 CCTCAGC CCTCAGC(-5/-2)
BcgI 506 GCATGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 651 GAGCAC G[AGT]GC[ACT]^C
BpmI 1144 CTCCAG CTGGAG(16/14)
BsaI 1231 GGTCTC GGTCTC(1/5)
BsaWI 888 TCCGGA [AT]^CCGG[AT]
BseAI 888 TCCGGA T^CCGGA
BseRI 436 GAGGAG GAGGAG(10/8)
BsgI 1159 CTGCAC GTGCAG(16/14)
BsiHKAI 651 GAGCAC G[AT]GC[AT]^C
BsmAI 1232 GTCTC GTCTC(1/5)
Bsp1286I 651 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 14 TGTACA T^GTACA
BspEI 888 TCCGGA T^CCGGA
BspHI 843 TCATGA T^CATGA
BsrBI 36 GAGCGG CCGCTC(-3/-3)
BsrGI 14 TGTACA T^GTACA
BstXI 224 CCAGAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 672 TTTAAA TTT^AAA
DraII 295 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1162 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 575 TGGCCA [CT]^GGCC[GA]
EcoNI 897 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 295 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 720 GAATTC G^AATTC
EcoRV 1282 GATATC GAT^ATC
EcoT22I 504 ATGCAT ATGCA^T
HapII 889 CCGG C^CGG
HindIII 951 AAGCTT A^AGCTT
HpaII 889 CCGG C^CGG
MluNI 575 TGGCCA TGG^CCA
MroI 888 TCCGGA T^CCGGA
MscI 575 TGGCCA TGG^CCA
MslI 129 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 889 CCGG C^CGG
NsiI 504 ATGCAT ATGCA^T
NspI 506 GCATGC [GA]CATG^[CT]
PflFI 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 295 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 571 CAGCTG CAG^CTG
RcaI 843 TCATGA T^CATGA
SphI 506 GCATGC GCATG^C
SspBI 14 TGTACA T^GTACA
TaqI 51 TCGA T^CGA
TfiI 91 GATTC G^A[AT]TC
Tth111I 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 51 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 15 GTAC GT^AC
938 GTAC
BbsI 489 GAAGAC GAAGAC(2/6)
976 GAAGAC
BpuAI 489 GAAGAC GAAGAC(2/6)
976 GAAGAC
BsrI 418 ACTGG ACTGG(1/-1)
1030 CCAGT
BstF5I 604 CATCC GGATG(2/0)
1176 GGATG
BstYI 667 GGATCT [GA]^GATC[CT]
1077 GGATCT
EarI 1129 GAAGAG CTCTTC(1/4)
1224 GAAGAG
FokI 604 CATCC GGATG(9/13)
1176 GGATG
Ksp632I 1129 GAAGAG CTCTTC(1/4)
1224 GAAGAG
MflI 667 GGATCT [GA]^GATC[CT]
1077 GGATCT
MspA1I 244 CAGCGG C[AC]G^C[GT]G
571 CAGCTG
PstI 740 CTGCAG CTGCA^G
753 CTGCAG
RsaI 15 GTAC GT^AC
938 GTAC
SfaNI 103 GATGC GCATC(5/9)
1009 GCATC
SmlI 583 CTCAAG C^T[CT][GA]AG
876 CTCAAG
SspI 75 AATATT AAT^ATT
1090 AATATT
XhoII 667 GGATCT [GA]^GATC[CT]
1077 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BsaJI BsiYI BslI
BsmI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI