Restriction map for
pFN21AE1366
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII AccI AccII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KspI MaeI
MfeI MluI MluNI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 254 GGTACC G^GTACC
AccIII 289 TCCGGA T^CCGGA
AfaI 255 GTAC GT^AC
ApaI 114 GGGCCC GGGCC^C
Asp700I 787 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 254 GGTACC G^GTACC
AvaII 76 GGTCC G^G[AT]CC
BanI 254 GGTACC G^G[CT][GA]CC
BcnI 36 CCCGG CC^[GC]GG
BsaBI 588 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 289 TCCGGA [AT]^CCGG[AT]
BseAI 289 TCCGGA T^CCGGA
BsmAI 826 GAGAC GTCTC(1/5)
BsmBI 826 GAGACG CGTCTC(1/5)
BsmFI 28 GGGAC GGGAC(10/14)
BspEI 289 TCCGGA T^CCGGA
BspHI 134 TCATGA T^CATGA
BsrDI 189 CATTGC GCAATG(2/0)
BsrI 108 ACTGG ACTGG(1/-1)
BstF5I 98 GGATG GGATG(2/0)
BstYI 224 GGATCT [GA]^GATC[CT]
Cac8I 625 GCTGGC GC[ACGT]^[ACGT]GC
DraI 438 TTTAAA TTT^AAA
EcoRI 357 GAATTC G^AATTC
FokI 98 GGATG GGATG(9/13)
HindIII 126 AAGCTT A^AGCTT
HphI 865 TCACC GGTGA(8/7)
KpnI 254 GGTACC GGTAC^C
MaeII 655 ACGT A^CGT
MamI 588 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 224 GGATCT [GA]^GATC[CT]
MroI 289 TCCGGA T^CCGGA
MslI 237 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 36 CCCGG CC^[GC]GG
PleI 450 GAGTC GAGTC(4/5)
RcaI 134 TCATGA T^CATGA
RsaI 255 GTAC GT^AC
SacI 467 GAGCTC GAGCT^C
SapI 180 GCTCTTC GCTCTTC(1/4)
SspI 418 AATATT AAT^ATT
Tsp45I 244 GTGAC ^GT[GC]AC
XhoII 224 GGATCT [GA]^GATC[CT]
XmnI 787 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 467 GAGCTC G[AT]GC[AT]^C
657 GTGCTC
BbvI 304 GCAGC GCAGC(8/12)
376 GCTGC
BpmI 321 CTCCAG CTGGAG(16/14)
660 CTCCAG
BsaJI 194 CCCTGG C^C[ACGT][ACGT]GG
195 CCTGGG
BsiHKAI 467 GAGCTC G[AT]GC[AT]^C
657 GTGCTC
DdeI 146 CTAAG C^T[ACGT]AG
518 CTGAG
DraII 54 AGGCCCT [GA]G^G[ACGT]CC[CT]
114 GGGCCCC
EcoO109I 54 AGGCCCT [GA]G^G[ACGT]CC[CT]
114 GGGCCCC
Fnu4HI 304 GCAGC GC^[ACGT]GC
376 GCTGC
HaeIII 55 GGCC GG^CC
115 GGCC
HapII 37 CCGG C^CGG
290 CCGG
HpaII 37 CCGG C^CGG
290 CCGG
ItaI 304 GCAGC GC^[ACGT]GC
376 GCTGC
MaeIII 244 GTGAC ^GT[ACGT]AC
548 GTTAC
MspI 37 CCGG C^CGG
290 CCGG
NlaIII 135 CATG CATG^
405 CATG
SfaNI 588 GATGC GCATC(5/9)
629 GCATC
TseI 304 GCAGC G^C[AT]GC
376 GCTGC
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
584 CAGTG
XcmI 165 CCAGATTATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
323 CCAGGAAGTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI AlwI ApoI BanII
BbsI BmyI BpuAI BsiYI BslI
Bsp1286I BssKI BstNI Cfr13I DpnII
EarI EcoRII HinfI Ksp632I MboI
MboII MnlI MseI MvaI NdeII
NlaIV Sau3AI Sau96I ScrFI TfiI
Tru9I Tsp509I