Restriction map for
pFN21AE1367
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NdeI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 108 CGCG CG^CG
AcyI 49 GGCGCC G[GA]^CG[CT]C
AfaI 806 GTAC GT^AC
AlwI 950 GGATC GGATC(4/5)
ApaI 65 GGGCCC GGGCC^C
AvaI 79 CTCGAG C^[CT]CG[GA]G
BbeI 49 GGCGCC GGCGC^C
BbsI 142 GAAGAC GAAGAC(2/6)
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
BpuAI 142 GAAGAC GAAGAC(2/6)
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsiEI 86 CGGCCG CG[GA][CT]^CG
BsmI 522 GCATTC GAATGC(1/-1)
BsoBI 79 CTCGAG C^[CT]CG[GA]G
BspMI 1076 ACCTGC ACCTGC(4/8)
BsrFI 88 GCCGGC [GA]^CCGG[CT]
BstUI 108 CGCG CG^CG
BstXI 344 CCAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 88 GCCGGC [GA]^CCGG[CT]
DraI 367 TTTAAA TTT^AAA
DsaI 992 CCATGG C^C[GA][CT]GG
EagI 86 CGGCCG C^GGCCG
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HincII 1104 GTTAAC GT[CT]^[GA]AC
HindII 1104 GTTAAC GT[CT]^[GA]AC
HpaI 1104 GTTAAC GTT^AAC
KasI 49 GGCGCC G^GCGCC
MspA1I 1099 CAGCGG C[AC]G^C[GT]G
MvnI 108 CGCG CG^CG
NaeI 88 GCCGGC GCC^GGC
NarI 49 GGCGCC GG^CGCC
NcoI 992 CCATGG C^CATGG
NgoMIV 88 GCCGGC G^CCGGC
PaeR7I 79 CTCGAG C^TCGAG
PflMI 621 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 806 GTAC GT^AC
SapI 851 GCTCTTC GCTCTTC(1/4)
SfaNI 979 GATGC GCATC(5/9)
SfcI 392 CTGTAG C^T[GA][CT]AG
SfoI 49 GGCGCC GGC^GCC
SmlI 79 CTCGAG C^T[CT][GA]AG
Van91I 621 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 344 CCAACGAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 79 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 896 AAATTC [GA]^AATT[CT]
931 AAATTT
ApoI 896 AAATTC [GA]^AATT[CT]
931 AAATTT
AvaII 60 GGACC G^G[AT]CC
708 GGACC
BcnI 63 CCGGG CC^[GC]GG
983 CCCGG
BfaI 112 CTAG C^TAG
673 CTAG
BglI 496 GCCTATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
820 GCCCAACAGGC
BseRI 55 GAGGAG GAGGAG(10/8)
256 GAGGAG
BsgI 925 GTGCAG GTGCAG(16/14)
1096 GTGCAG
BspHI 237 TCATGA T^CATGA
1016 TCATGA
EaeI 86 CGGCCG [CT]^GGCC[GA]
985 CGGCCA
EcoT14I 468 CCTTGG C^C[AT][AT]GG
992 CCATGG
FauI 68 CCCGC CCCGC(4/6)
914 GCGGG
HaeII 21 GGCGCT [GA]GCGC^[CT]
49 GGCGCC
HphI 42 GGTGA GGTGA(8/7)
690 GGTGA
MaeI 112 CTAG C^TAG
673 CTAG
NciI 63 CCGGG CC^[GC]GG
983 CCCGG
NheI 111 GCTAGC G^CTAGC
672 GCTAGC
RcaI 237 TCATGA T^CATGA
1016 TCATGA
StyI 468 CCTTGG C^C[AT][AT]GG
992 CCATGG
TaqI 80 TCGA T^CGA
866 TCGA
TfiI 569 GATTC G^A[AT]TC
871 GAATC
Tsp509I 897 AATT ^AATT
932 AATT
TthHB8I 80 TCGA T^CGA
866 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwNI BanI BanII
BbvI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp45I TspRI