Restriction map for
pFN21AE6437
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspI
AvaI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT14I EcoT22I FauI
FbaI FseI FspI HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KspI MamI MfeI MroI
MspA1I MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 649 GGTACC G^GTACC
AccII 556 CGCG CG^CG
AciI 1413 CCGC CCGC(-3/-1)
AflIII 555 ACGCGT A^C[GA][CT]GT
AhdI 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 505 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1422 GGGCCC GGGCC^C
Asp718I 649 GGTACC G^GTACC
AspEI 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BciVI 869 GTATCC GTATCC(6/5)
BlpI 283 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 283 GCTGAGC GC^T[ACGT]AGC
BsgI 330 GTGCAG GTGCAG(16/14)
Bsp1407I 1033 TGTACA T^GTACA
BspMI 85 ACCTGC ACCTGC(4/8)
BsrGI 1033 TGTACA T^GTACA
BstUI 556 CGCG CG^CG
CelII 283 GCTGAGC GC^T[ACGT]AGC
CfoI 666 GCGC GCG^C
DraIII 324 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eam1105I 1636 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 485 GAATTC G^AATTC
EcoRV 1306 GATATC GAT^ATC
HaeII 665 GGCGCT [GA]GCGC^[CT]
HhaI 666 GCGC GCG^C
HinP1I 666 GCGC G^CGC
KpnI 649 GGTACC GGTAC^C
MaeII 747 ACGT A^CGT
MluI 555 ACGCGT A^CGCGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 594 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 556 CGCG CG^CG
PleI 1181 GACTC GAGTC(4/5)
PpuMI 71 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1144 GAGCTC GAGCT^C
SapI 668 GCTCTTC GCTCTTC(1/4)
ScaI 637 AGTACT AGT^ACT
SfiI 1105 GGCCTTATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1354 CTCAAG C^T[CT][GA]AG
SspBI 1033 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 22 enzymes) #
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Asp700I 472 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
538 GAAGGCTTTC
AspHI 286 GAGCAC G[AT]GC[AT]^C
1144 GAGCTC
AvaII 72 GGACC G^G[AT]CC
1390 GGACC
BamHI 1123 GGATCC G^GATCC
1359 GGATCC
BanI 649 GGTACC G^G[CT][GA]CC
1557 GGCACC
BpmI 76 CTGGAG CTGGAG(16/14)
584 CTGGAG
BseRI 45 CTCCTC GAGGAG(10/8)
587 GAGGAG
BsiHKAI 286 GAGCAC G[AT]GC[AT]^C
1144 GAGCTC
BsmAI 388 GAGAC GTCTC(1/5)
590 GAGAC
BspHI 242 TCATGA T^CATGA
795 TCATGA
BsrDI 594 CATTGC GCAATG(2/0)
1290 CATTGC
DraII 71 AGGACCT [GA]G^G[ACGT]CC[CT]
1422 GGGCCCT
EarI 669 CTCTTC CTCTTC(1/4)
1577 CTCTTC
EcoO109I 71 AGGACCT [GA]G^G[ACGT]CC[CT]
1422 GGGCCCT
HgaI 345 GCGTC GACGC(5/10)
557 GCGTC
HphI 564 GGTGA GGTGA(8/7)
1617 GGTGA
Ksp632I 669 CTCTTC CTCTTC(1/4)
1577 CTCTTC
PshAI 153 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
177 GACAAATGTC
RcaI 242 TCATGA T^CATGA
795 TCATGA
TaqI 612 TCGA T^CGA
1497 TCGA
TthHB8I 612 TCGA T^CGA
1497 TCGA
XmnI 472 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
538 GAAGGCTTTC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
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AcsI AfaI AluI AlwI ApoI
BanII BbvI BfaI BglI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII