Restriction map for
pFN21AE5616
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CfoI ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI MaeI MamI
MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 293 GGTACC G^GTACC
AccI 1166 GTCGAC GT^[AC][GT]AC
AccII 9 CGCG CG^CG
AocI 1371 CCTCAGG CC^T[ACGT]AGG
ApaI 494 GGGCCC GGGCC^C
Asp718I 293 GGTACC G^GTACC
AvaII 5 GGACC G^G[AT]CC
BalI 1360 TGGCCA TGG^CCA
BamHI 1465 GGATCC G^GATCC
BanI 293 GGTACC G^G[CT][GA]CC
BbvCI 262 GCTGAGG CCTCAGC(-5/-2)
BciVI 1389 GGATAC GTATCC(6/5)
BcnI 820 CCGGG CC^[GC]GG
BsaI 386 GAGACC GGTCTC(1/5)
BspMI 1455 GCAGGT ACCTGC(4/8)
BsrFI 159 GCCGGT [GA]^CCGG[CT]
BstEII 621 GGTCACC G^GT[ACGT]ACC
BstPI 621 GGTCACC G^GT[ACGT]ACC
BstUI 9 CGCG CG^CG
Bsu36I 1371 CCTCAGG CC^T[ACGT]AGG
Cfr10I 159 GCCGGT [GA]^CCGG[CT]
EaeI 1360 TGGCCA [CT]^GGCC[GA]
Eco81I 1371 CCTCAGG CC^T[ACGT]AGG
EcoNI 327 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 621 GGTCACC G^GT[ACGT]ACC
EcoT14I 933 CCAAGG C^C[AT][AT]GG
EcoT22I 207 ATGCAT ATGCA^T
HgaI 1417 GCGTC GACGC(5/10)
HincII 1166 GTCGAC GT[CT]^[GA]AC
HindII 1166 GTCGAC GT[CT]^[GA]AC
HinfI 808 GACTC G^A[ACGT]TC
KpnI 293 GGTACC GGTAC^C
KspI 8 CCGCGG CCGC^GG
MfeI 1268 CAATTG C^AATTG
MluNI 1360 TGGCCA TGG^CCA
MscI 1360 TGGCCA TGG^CCA
MseI 1090 TTAA T^TAA
MunI 1268 CAATTG C^AATTG
MvnI 9 CGCG CG^CG
NciI 820 CCGGG CC^[GC]GG
NsiI 207 ATGCAT ATGCA^T
PleI 808 GACTC GAGTC(4/5)
SacII 8 CCGCGG CCGC^GG
SalI 1166 GTCGAC G^TCGAC
SapI 961 GAAGAGC GCTCTTC(1/4)
SexAI 836 ACCAGGT A^CC[AT]GGT
StuI 137 AGGCCT AGG^CCT
StyI 933 CCAAGG C^C[AT][AT]GG
TaqI 1167 TCGA T^CGA
Tru9I 1090 TTAA T^TAA
TthHB8I 1167 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 811 TCCGGA T^CCGGA
1448 TCCGGA
AspHI 1028 GAGCAC G[AT]GC[AT]^C
1440 GAGCAC
BanII 494 GGGCCC G[GA]GC[CT]^C
964 GAGCCC
BbsI 559 GAAGAC GAAGAC(2/6)
481 GTCTTC
BlpI 22 GCTCAGC GC^T[ACGT]AGC
1335 GCTAAGC
Bpu1102I 22 GCTCAGC GC^T[ACGT]AGC
1335 GCTAAGC
BpuAI 559 GAAGAC GAAGAC(2/6)
481 GTCTTC
BseAI 811 TCCGGA T^CCGGA
1448 TCCGGA
BsiHKAI 1028 GAGCAC G[AT]GC[AT]^C
1440 GAGCAC
BspEI 811 TCCGGA T^CCGGA
1448 TCCGGA
BstYI 1288 AGATCC [GA]^GATC[CT]
1465 GGATCC
CelII 22 GCTCAGC GC^T[ACGT]AGC
1335 GCTAAGC
DraIII 419 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
835 CACCAGGTG
DsaI 8 CCGCGG C^C[GA][CT]GG
867 CCGTGG
EarI 1312 CTCTTC CTCTTC(1/4)
961 GAAGAG
Ksp632I 1312 CTCTTC CTCTTC(1/4)
961 GAAGAG
MaeII 764 ACGT A^CGT
920 ACGT
MflI 1288 AGATCC [GA]^GATC[CT]
1465 GGATCC
MroI 811 TCCGGA T^CCGGA
1448 TCCGGA
PflMI 862 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1190 CCATCAGCTGG
PstI 608 CTGCAG CTGCA^G
1099 CTGCAG
SmlI 451 CTTGAG C^T[CT][GA]AG
595 CTTGAG
Van91I 862 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1190 CCATCAGCTGG
XhoII 1288 AGATCC [GA]^GATC[CT]
1465 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BmyI BpmI BsaJI BsaWI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I Tsp509I TspRI
XcmI