Restriction map for
pFN21AE6207
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BciVI BclI BfaI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MseI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 772 GTCTAC GT^[AC][GT]AC
AflIII 794 ACATGT A^C[GA][CT]GT
AlwI 803 GGATC GGATC(4/5)
BalI 428 TGGCCA TGG^CCA
BbsI 607 GTCTTC GAAGAC(2/6)
BcgI 53 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 88 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 782 AGATCT A^GATCT
BlpI 487 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 487 GCTCAGC GC^T[ACGT]AGC
BpuAI 607 GTCTTC GAAGAC(2/6)
BspLU11I 794 ACATGT A^CATGT
BssSI 729 CTCGTG CACGAG(-5/-1)
BstEII 624 GGTCACC G^GT[ACGT]ACC
BstPI 624 GGTCACC G^GT[ACGT]ACC
BstXI 507 CCAGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 782 AGATCT [GA]^GATC[CT]
CelII 487 GCTCAGC GC^T[ACGT]AGC
DsaI 527 CCACGG C^C[GA][CT]GG
EarI 187 CTCTTC CTCTTC(1/4)
EcoO65I 624 GGTCACC G^GT[ACGT]ACC
HgaI 455 GCGTC GACGC(5/10)
HphI 626 TCACC GGTGA(8/7)
Ksp632I 187 CTCTTC CTCTTC(1/4)
MflI 782 AGATCT [GA]^GATC[CT]
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
NspI 794 ACATGT [GA]CATG^[CT]
PaeR7I 281 CTCGAG C^TCGAG
PpuMI 665 AGGACCC [GA]G^G[AT]CC[CT]
PstI 466 CTGCAG CTGCA^G
PvuII 767 CAGCTG CAG^CTG
SacI 5 GAGCTC GAGCT^C
SapI 186 GCTCTTC GCTCTTC(1/4)
SfcI 466 CTGCAG C^T[GA][CT]AG
SmaI 41 CCCGGG CCC^GGG
SmlI 281 CTCGAG C^T[CT][GA]AG
TaqI 282 TCGA T^CGA
TthHB8I 282 TCGA T^CGA
XcmI 675 CCAGAGTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 281 CTCGAG C^TCGAG
XhoII 782 AGATCT [GA]^GATC[CT]
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 37 GGCGCC G[GA]^CG[CT]C
145 GGCGCC
AfaI 360 GTAC GT^AC
549 GTAC
ApaI 448 GGGCCC GGGCC^C
461 GGGCCC
AvaI 41 CCCGGG C^[CT]CG[GA]G
281 CTCGAG
BbeI 37 GGCGCC GGCGC^C
145 GGCGCC
BpmI 570 CTCCAG CTGGAG(16/14)
686 CTGGAG
BsaHI 37 GGCGCC G[GA]^CG[CT]C
145 GGCGCC
BsiEI 248 CGGCCG CG[GA][CT]^CG
530 CGGCCG
BsoBI 41 CCCGGG C^[CT]CG[GA]G
281 CTCGAG
BsrI 651 ACTGG ACTGG(1/-1)
501 CCAGT
BssHII 155 GCGCGC G^CGCGC
157 GCGCGC
EagI 248 CGGCCG C^GGCCG
530 CGGCCG
EclXI 248 CGGCCG C^GGCCG
530 CGGCCG
Eco52I 248 CGGCCG C^GGCCG
530 CGGCCG
EcoT14I 388 CCAAGG C^C[AT][AT]GG
717 CCAAGG
FauI 452 CCCGC CCCGC(4/6)
214 GCGGG
Hin1I 37 GGCGCC G[GA]^CG[CT]C
145 GGCGCC
HinfI 336 GACTC G^A[ACGT]TC
682 GACTC
KasI 37 GGCGCC G^GCGCC
145 GGCGCC
NarI 37 GGCGCC GG^CGCC
145 GGCGCC
PleI 336 GACTC GAGTC(4/5)
682 GACTC
RsaI 360 GTAC GT^AC
549 GTAC
SfoI 37 GGCGCC GGC^GCC
145 GGCGCC
StyI 388 CCAAGG C^C[AT][AT]GG
717 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI