Restriction map for
pFN21AE4530
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmI
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MluI MluNI MscI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 631 GGTACC G^GTACC
AccIII 677 TCCGGA T^CCGGA
AflIII 1476 ACGTGT A^C[GA][CT]GT
Asp718I 631 GGTACC G^GTACC
AspI 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 748 CTCGAG C^[CT]CG[GA]G
BanI 631 GGTACC G^G[CT][GA]CC
BanII 201 GGGCTC G[GA]GC[CT]^C
BbsI 1099 GTCTTC GAAGAC(2/6)
BcgI 368 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1099 GTCTTC GAAGAC(2/6)
BsaBI 458 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 677 TCCGGA [AT]^CCGG[AT]
BseAI 677 TCCGGA T^CCGGA
BseRI 1563 GAGGAG GAGGAG(10/8)
BsmFI 1073 GGGAC GGGAC(10/14)
BsoBI 748 CTCGAG C^[CT]CG[GA]G
Bsp1407I 380 TGTACA T^GTACA
BspEI 677 TCCGGA T^CCGGA
BsrGI 380 TGTACA T^GTACA
BstAPI 1518 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1281 TTCGAA TT^CGAA
DraII 1642 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 206 CCACGG C^C[GA][CT]GG
EaeI 353 TGGCCG [CT]^GGCC[GA]
EcoO109I 1642 AGGACCC [GA]G^G[ACGT]CC[CT]
HapII 678 CCGG C^CGG
HincII 862 GTCAAC GT[CT]^[GA]AC
HindII 862 GTCAAC GT[CT]^[GA]AC
HindIII 736 AAGCTT A^AGCTT
HpaII 678 CCGG C^CGG
KpnI 631 GGTACC GGTAC^C
MamI 458 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 125 CAATTG C^AATTG
MroI 677 TCCGGA T^CCGGA
MslI 372 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 678 CCGG C^CGG
MunI 125 CAATTG C^AATTG
NspI 1762 GCATGT [GA]CATG^[CT]
NspV 1281 TTCGAA TT^CGAA
PaeR7I 748 CTCGAG C^TCGAG
PflFI 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1642 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 894 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 766 GAAGAGC GCTCTTC(1/4)
ScaI 1337 AGTACT AGT^ACT
SfuI 1281 TTCGAA TT^CGAA
SmlI 748 CTCGAG C^T[CT][GA]AG
SspBI 380 TGTACA T^GTACA
Tth111I 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 526 CCAGACTTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 748 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BciVI 1361 GGATAC GTATCC(6/5)
674 GTATCC
BmyI 201 GGGCTC G[AGT]GC[ACT]^C
247 GTGCCC
BsmBI 1300 CGTCTC CGTCTC(1/5)
1749 GAGACG
Bsp1286I 201 GGGCTC G[AGT]GC[ACT]^C
247 GTGCCC
BspMI 659 ACCTGC ACCTGC(4/8)
1064 ACCTGC
BsrI 635 CCAGT ACTGG(1/-1)
1620 CCAGT
EarI 766 GAAGAG CTCTTC(1/4)
1372 GAAGAG
EcoT14I 953 CCTTGG C^C[AT][AT]GG
1400 CCAAGG
FauI 1173 GCGGG CCCGC(4/6)
1270 GCGGG
Ksp632I 766 GAAGAG CTCTTC(1/4)
1372 GAAGAG
MaeII 899 ACGT A^CGT
1476 ACGT
MaeIII 320 GTGAC ^GT[ACGT]AC
433 GTGAC
MseI 656 TTAA T^TAA
1655 TTAA
PstI 977 CTGCAG CTGCA^G
1381 CTGCAG
SfcI 977 CTGCAG C^T[GA][CT]AG
1381 CTGCAG
StyI 953 CCTTGG C^C[AT][AT]GG
1400 CCAAGG
TaqI 749 TCGA T^CGA
1282 TCGA
Tru9I 656 TTAA T^TAA
1655 TTAA
Tsp45I 320 GTGAC ^GT[GC]AC
433 GTGAC
TthHB8I 749 TCGA T^CGA
1282 TCGA
XbaI 876 TCTAGA T^CTAGA
1251 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbvI
BfaI BpmI BsaJI BsgI BsiYI
BslI BsmAI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI XhoII
XmnI