Restriction map for
pFN21AE1319
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 231 ACGTGT A^C[GA][CT]GT
AlwNI 94 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 393 GGGCCC GGGCC^C
Asp700I 880 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 341 CCCGAG C^[CT]CG[GA]G
AviII 711 TGCGCA TGC^GCA
BanII 393 GGGCCC G[GA]GC[CT]^C
BglI 24 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 555 AGATCT A^GATCT
BsaAI 699 CACGTA [CT]AC^GT[GA]
BseRI 256 GAGGAG GAGGAG(10/8)
BsiEI 144 CGACCG CG[GA][CT]^CG
BsoBI 341 CCCGAG C^[CT]CG[GA]G
BsrBI 55 GAGCGG CCGCTC(-3/-3)
BssHII 187 GCGCGC G^CGCGC
BstBI 845 TTCGAA TT^CGAA
EaeI 8 TGGCCG [CT]^GGCC[GA]
EcoRI 208 GAATTC G^AATTC
EcoT14I 308 CCAAGG C^C[AT][AT]GG
FspI 711 TGCGCA TGC^GCA
HaeII 570 GGCGCT [GA]GCGC^[CT]
HincII 472 GTTGAC GT[CT]^[GA]AC
HindII 472 GTTGAC GT[CT]^[GA]AC
MaeIII 737 GTGAC ^GT[ACGT]AC
MseI 920 TTAA T^TAA
MslI 699 CACGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 179 GCATGC [GA]CATG^[CT]
NspV 845 TTCGAA TT^CGAA
PflMI 397 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 91 CTGCAG CTGCA^G
PvuII 631 CAGCTG CAG^CTG
SfuI 845 TTCGAA TT^CGAA
SphI 179 GCATGC GCATG^C
StyI 308 CCAAGG C^C[AT][AT]GG
Tru9I 920 TTAA T^TAA
Tsp45I 737 GTGAC ^GT[GC]AC
Van91I 397 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 880 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 208 GAATTC [GA]^AATT[CT]
797 AAATTC
AcyI 61 GACGCC G[GA]^CG[CT]C
130 GGCGTC
ApoI 208 GAATTC [GA]^AATT[CT]
797 AAATTC
BamHI 280 GGATCC G^GATCC
337 GGATCC
BanI 154 GGCACC G^G[CT][GA]CC
160 GGCACC
BbsI 880 GAAGAC GAAGAC(2/6)
303 GTCTTC
BmyI 153 GGGCAC G[AGT]GC[ACT]^C
393 GGGCCC
BpuAI 880 GAAGAC GAAGAC(2/6)
303 GTCTTC
BsaHI 61 GACGCC G[GA]^CG[CT]C
130 GGCGTC
BsmAI 344 GAGAC GTCTC(1/5)
650 GAGAC
BsmFI 59 GGGAC GGGAC(10/14)
386 GGGAC
Bsp1286I 153 GGGCAC G[AGT]GC[ACT]^C
393 GGGCCC
DdeI 199 CTGAG C^T[ACGT]AG
835 CTCAG
FauI 43 CCCGC CCCGC(4/6)
57 GCGGG
Hin1I 61 GACGCC G[GA]^CG[CT]C
130 GGCGTC
HphI 609 GGTGA GGTGA(8/7)
736 GGTGA
MaeII 231 ACGT A^CGT
700 ACGT
MspA1I 123 CCGCTG C[AC]G^C[GT]G
631 CAGCTG
NaeI 10 GCCGGC GCC^GGC
29 GCCGGC
NgoMIV 10 GCCGGC G^CCGGC
29 GCCGGC
SfcI 91 CTGCAG C^T[GA][CT]AG
192 CTACAG
TaqI 785 TCGA T^CGA
846 TCGA
TthHB8I 785 TCGA T^CGA
846 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BsaJI BsiYI BslI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI XhoII