Restriction map for
pFN21AE5602
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BciVI BclI
BfrI BglI BglII BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 1441 ACATGT A^C[GA][CT]GT
AocI 476 CCTTAGG CC^T[ACGT]AGG
AseI 1183 ATTAAT AT^TAAT
AsnI 1183 ATTAAT AT^TAAT
AspHI 435 GAGCTC G[AT]GC[AT]^C
AvrII 330 CCTAGG C^CTAGG
BbvCI 910 CCTCAGC CCTCAGC(-5/-2)
BcgI 1073 GCAGTGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1230 CCGGG CC^[GC]GG
BlnI 330 CCTAGG C^CTAGG
BpmI 680 CTCCAG CTGGAG(16/14)
BsiHKAI 435 GAGCTC G[AT]GC[AT]^C
Bsp1407I 66 TGTACA T^GTACA
BspLU11I 1441 ACATGT A^CATGT
BsrGI 66 TGTACA T^GTACA
BstAPI 139 GCATATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 476 CCTTAGG CC^T[ACGT]AGG
DraIII 1156 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 29 GACCAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1068 CCGTGG C^C[GA][CT]GG
Eco81I 476 CCTTAGG CC^T[ACGT]AGG
EcoNI 525 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 137 ATGCAT ATGCA^T
HapII 1230 CCGG C^CGG
HincII 1381 GTCAAC GT[CT]^[GA]AC
HindII 1381 GTCAAC GT[CT]^[GA]AC
HindIII 1171 AAGCTT A^AGCTT
HpaII 1230 CCGG C^CGG
MspI 1230 CCGG C^CGG
NciI 1230 CCGGG CC^[GC]GG
NheI 727 GCTAGC G^CTAGC
NsiI 137 ATGCAT ATGCA^T
NspI 1441 ACATGT [GA]CATG^[CT]
PleI 114 GAGTC GAGTC(4/5)
PpuMI 843 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1183 ATTAAT AT^TAAT
PvuII 271 CAGCTG CAG^CTG
SacI 435 GAGCTC GAGCT^C
SmlI 111 CTTGAG C^T[CT][GA]AG
SpeI 649 ACTAGT A^CTAGT
SspBI 66 TGTACA T^GTACA
SspI 1288 AATATT AAT^ATT
XcmI 8 CCAGCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 225 GTCTAC GT^[AC][GT]AC
426 GTCTAC
AfaI 52 GTAC GT^AC
67 GTAC
AlwI 61 GGATC GGATC(4/5)
1135 GGATC
BbsI 759 GAAGAC GAAGAC(2/6)
788 GTCTTC
BpuAI 759 GAAGAC GAAGAC(2/6)
788 GTCTTC
BseRI 695 CTCCTC GAGGAG(10/8)
908 CTCCTC
BsgI 1296 GTGCAG GTGCAG(16/14)
1445 GTGCAG
BspMI 1447 GCAGGT ACCTGC(4/8)
1614 GCAGGT
BstXI 706 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1042 CCAACCCGCTGG
BstYI 61 GGATCT [GA]^GATC[CT]
1135 GGATCT
DpnII 62 GATC ^GATC
1136 GATC
DraII 843 GGGTCCC [GA]G^G[ACGT]CC[CT]
1356 AGGCCCC
EaeI 5 CGGCCA [CT]^GGCC[GA]
1227 TGGCCG
EcoO109I 843 GGGTCCC [GA]G^G[ACGT]CC[CT]
1356 AGGCCCC
EcoT14I 330 CCTAGG C^C[AT][AT]GG
558 CCAAGG
FauI 1046 CCCGC CCCGC(4/6)
638 GCGGG
MaeII 1511 ACGT A^CGT
1719 ACGT
MboI 62 GATC ^GATC
1136 GATC
MflI 61 GGATCT [GA]^GATC[CT]
1135 GGATCT
MslI 240 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1396 CACTTCTGTG
MspA1I 271 CAGCTG C[AC]G^C[GT]G
1047 CCGCTG
NdeII 62 GATC ^GATC
1136 GATC
PflMI 343 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
558 CCAAGGGATGG
RsaI 52 GTAC GT^AC
67 GTAC
Sau3AI 62 GATC ^GATC
1136 GATC
StyI 330 CCTAGG C^C[AT][AT]GG
558 CCAAGG
TfiI 157 GATTC G^A[AT]TC
1215 GAATC
Tsp45I 236 GTGAC ^GT[GC]AC
1567 GTGAC
Van91I 343 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
558 CCAAGGGATGG
XhoII 61 GGATCT [GA]^GATC[CT]
1135 GGATCT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BfaI BlpI BmyI Bpu1102I BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp509I TspRI