Restriction map for
pFN21AE4461
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BbrPI BbsI BciVI BclI BfrI
BglII BpuAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstF5I BstPI BstUI
ClaI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
Sse8387I SspBI SspI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1263 GTATAC GT^[AC][GT]AC
AfaI 960 GTAC GT^AC
Alw44I 436 GTGCAC G^TGCAC
AlwI 1018 GATCC GGATC(4/5)
Aor51HI 665 AGCGCT AGC^GCT
ApaLI 436 GTGCAC G^TGCAC
Asp700I 285 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 198 CCTAGG C^CTAGG
BbeI 1388 GGCGCC GGCGC^C
BcgI 573 GCAAACCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 198 CCTAGG C^CTAGG
BsaAI 298 TACGTG [CT]AC^GT[GA]
BsaI 266 GAGACC GGTCTC(1/5)
BsmBI 476 CGTCTC CGTCTC(1/5)
BsmFI 818 GGGAC GGGAC(10/14)
BsmI 1253 GCATTC GAATGC(1/-1)
BsrFI 765 GCCGGT [GA]^CCGG[CT]
BssSI 908 CTCGTG CACGAG(-5/-1)
Bst1107I 1263 GTATAC GTA^TAC
BstYI 1017 AGATCC [GA]^GATC[CT]
BstZ17I 1263 GTATAC GTA^TAC
Cfr10I 765 GCCGGT [GA]^CCGG[CT]
CpoI 7 CGGACCG CG^G[AT]CCG
DpnII 1018 GATC ^GATC
DraIII 683 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 44 TGGCCG [CT]^GGCC[GA]
EarI 559 CTCTTC CTCTTC(1/4)
Eco47III 665 AGCGCT AGC^GCT
KasI 1388 GGCGCC G^GCGCC
Ksp632I 559 CTCTTC CTCTTC(1/4)
MboI 1018 GATC ^GATC
MflI 1017 AGATCC [GA]^GATC[CT]
MseI 1367 TTAA T^TAA
MslI 927 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1388 GGCGCC GG^CGCC
NcoI 485 CCATGG C^CATGG
NdeII 1018 GATC ^GATC
NspI 315 GCATGC [GA]CATG^[CT]
PpuMI 541 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 960 GTAC GT^AC
RsrII 7 CGGACCG CG^G[AT]CCG
SacI 529 GAGCTC GAGCT^C
Sau3AI 1018 GATC ^GATC
SfoI 1388 GGCGCC GGC^GCC
SmaI 410 CCCGGG CCC^GGG
SpeI 733 ACTAGT A^CTAGT
SphI 315 GCATGC GCATG^C
StuI 653 AGGCCT AGG^CCT
TaqI 138 TCGA T^CGA
Tru9I 1367 TTAA T^TAA
TthHB8I 138 TCGA T^CGA
XbaI 696 TCTAGA T^CTAGA
XhoII 1017 AGATCC [GA]^GATC[CT]
XmaCI 410 CCCGGG C^CCGGG
XmaI 410 CCCGGG C^CCGGG
XmnI 285 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 474 GGCGTC G[GA]^CG[CT]C
1388 GGCGCC
AvaI 173 CTCGGG C^[CT]CG[GA]G
410 CCCGGG
BbvCI 390 GCTGAGG CCTCAGC(-5/-2)
889 GCTGAGG
BsaHI 474 GGCGTC G[GA]^CG[CT]C
1388 GGCGCC
BsoBI 173 CTCGGG C^[CT]CG[GA]G
410 CCCGGG
BstXI 587 CCATATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCAGGGATTTGG
FauI 190 GCGGG CCCGC(4/6)
1173 GCGGG
HgaI 111 GCGTC GACGC(5/10)
475 GCGTC
Hin1I 474 GGCGTC G[GA]^CG[CT]C
1388 GGCGCC
HphI 948 GGTGA GGTGA(8/7)
355 TCACC
MaeII 299 ACGT A^CGT
842 ACGT
NlaIII 316 CATG CATG^
486 CATG
PflMI 651 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCACTGCTTGG
PshAI 9 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
820 GACAGCAGTC
PstI 929 CTGCAG CTGCA^G
955 CTGCAG
PvuII 275 CAGCTG CAG^CTG
1187 CAGCTG
Van91I 651 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCACTGCTTGG
XcmI 450 CCAGCGCCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
762 CCAGCCGGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwNI AocI ApaI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DraII DsaI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV PleI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI