Restriction map for
pFN21AE6418
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsgI BsiEI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstPI Bsu36I CelII
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 1541 TCCGGA T^CCGGA
ApaI 1288 GGGCCC GGGCC^C
AspI 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 14 TGGCCA TGG^CCA
BclI 930 TGATCA T^GATCA
BcnI 1058 CCCGG CC^[GC]GG
BglII 1425 AGATCT A^GATCT
BsaWI 1541 TCCGGA [AT]^CCGG[AT]
BseAI 1541 TCCGGA T^CCGGA
BsmBI 840 CGTCTC CGTCTC(1/5)
BspEI 1541 TCCGGA T^CCGGA
BspHI 540 TCATGA T^CATGA
BsrDI 1023 CATTGC GCAATG(2/0)
BssSI 1271 CACGAG CACGAG(-5/-1)
Bst1107I 1212 GTATAC GTA^TAC
BstZ17I 1212 GTATAC GTA^TAC
DraI 354 TTTAAA TTT^AAA
EaeI 14 TGGCCA [CT]^GGCC[GA]
EcoRV 1642 GATATC GAT^ATC
EcoT14I 170 CCAAGG C^C[AT][AT]GG
FauI 504 GCGGG CCCGC(4/6)
FbaI 930 TGATCA T^GATCA
HaeII 581 AGCGCC [GA]GCGC^[CT]
MaeII 1865 ACGT A^CGT
MluNI 14 TGGCCA TGG^CCA
MroI 1541 TCCGGA T^CCGGA
MscI 14 TGGCCA TGG^CCA
NciI 1058 CCCGG CC^[GC]GG
PflFI 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1156 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1855 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1414 CAGCTG CAG^CTG
RcaI 540 TCATGA T^CATGA
SacI 571 GAGCTC GAGCT^C
SalI 493 GTCGAC G^TCGAC
SapI 1611 GCTCTTC GCTCTTC(1/4)
ScaI 247 AGTACT AGT^ACT
SfcI 1087 CTACAG C^T[GA][CT]AG
StyI 170 CCAAGG C^C[AT][AT]GG
Tth111I 1137 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1156 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1245 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 493 GTCGAC GT^[AC][GT]AC
1212 GTATAC
AccII 626 CGCG CG^CG
947 CGCG
AcsI 1249 GAATTT [GA]^AATT[CT]
1745 AAATTT
AcyI 223 GGCGTC G[GA]^CG[CT]C
1657 GACGCC
ApoI 1249 GAATTT [GA]^AATT[CT]
1745 AAATTT
AvaI 209 CTCGAG C^[CT]CG[GA]G
443 CTCGAG
BbsI 942 GAAGAC GAAGAC(2/6)
1080 GAAGAC
BpuAI 942 GAAGAC GAAGAC(2/6)
1080 GAAGAC
BsaHI 223 GGCGTC G[GA]^CG[CT]C
1657 GACGCC
BsoBI 209 CTCGAG C^[CT]CG[GA]G
443 CTCGAG
BspMI 654 ACCTGC ACCTGC(4/8)
1731 GCAGGT
BsrBI 502 GAGCGG CCGCTC(-3/-3)
529 GAGCGG
BstUI 626 CGCG CG^CG
947 CGCG
HapII 1059 CCGG C^CGG
1542 CCGG
Hin1I 223 GGCGTC G[GA]^CG[CT]C
1657 GACGCC
HincII 493 GTCGAC GT[CT]^[GA]AC
1308 GTCAAC
HindII 493 GTCGAC GT[CT]^[GA]AC
1308 GTCAAC
HinfI 441 GACTC G^A[ACGT]TC
1721 GACTC
HpaII 1059 CCGG C^CGG
1542 CCGG
MspA1I 61 CAGCGG C[AC]G^C[GT]G
1414 CAGCTG
MspI 1059 CCGG C^CGG
1542 CCGG
MvnI 626 CGCG CG^CG
947 CGCG
NspI 890 ACATGC [GA]CATG^[CT]
1607 ACATGC
PaeR7I 209 CTCGAG C^TCGAG
443 CTCGAG
PleI 441 GACTC GAGTC(4/5)
1721 GACTC
SfiI 1056 GGCCCGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1581 GGCCTGGCTGGCC
XcmI 1628 CCACCAATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1652 CCATCGACGCCCTGG
XhoI 209 CTCGAG C^TCGAG
443 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HgaI
HhaI HinP1I HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII