Restriction map for
pFN21AE5406
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbsI BcgI
BfaI BfrI BglII BlnI BpmI
BpuAI BsaBI BsaI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoT14I EcoT22I FseI FspI
HaeII HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 205 GTCTAC GT^[AC][GT]AC
AccIII 542 TCCGGA T^CCGGA
AcsI 462 GAATTC [GA]^AATT[CT]
AcyI 117 GACGCC G[GA]^CG[CT]C
AfaI 579 GTAC GT^AC
AflIII 575 ACACGT A^C[GA][CT]GT
Alw44I 841 GTGCAC G^TGCAC
AlwNI 710 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 24 GGGCCC GGGCC^C
ApaLI 841 GTGCAC G^TGCAC
ApoI 462 GAATTC [GA]^AATT[CT]
AspHI 841 GTGCAC G[AT]GC[AT]^C
BamHI 232 GGATCC G^GATCC
BbvCI 490 CCTCAGC CCTCAGC(-5/-2)
BciVI 936 GGATAC GTATCC(6/5)
BclI 615 TGATCA T^GATCA
BglI 974 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 908 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 908 GCTCAGC GC^T[ACGT]AGC
BsaAI 576 CACGTA [CT]AC^GT[GA]
BsaHI 117 GACGCC G[GA]^CG[CT]C
BsaWI 542 TCCGGA [AT]^CCGG[AT]
BseAI 542 TCCGGA T^CCGGA
BsgI 501 CTGCAC GTGCAG(16/14)
BsiHKAI 841 GTGCAC G[AT]GC[AT]^C
BsmAI 1047 GAGAC GTCTC(1/5)
BspEI 542 TCCGGA T^CCGGA
BspMI 662 ACCTGC ACCTGC(4/8)
BsrBI 80 GAGCGG CCGCTC(-3/-3)
BsrDI 760 GCAATG GCAATG(2/0)
BstAPI 63 GCATGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 878 GGTCACC G^GT[ACGT]ACC
BstPI 878 GGTCACC G^GT[ACGT]ACC
BstXI 755 CCAATGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 232 GGATCC [GA]^GATC[CT]
CelII 908 GCTCAGC GC^T[ACGT]AGC
CfoI 666 GCGC GCG^C
DsaI 395 CCGTGG C^C[GA][CT]GG
EcoO65I 878 GGTCACC G^GT[ACGT]ACC
EcoRI 462 GAATTC G^AATTC
EcoRV 646 GATATC GAT^ATC
FbaI 615 TGATCA T^GATCA
HgaI 117 GACGC GACGC(5/10)
HhaI 666 GCGC GCG^C
Hin1I 117 GACGCC G[GA]^CG[CT]C
HinP1I 666 GCGC G^CGC
HindIII 529 AAGCTT A^AGCTT
MaeII 577 ACGT A^CGT
MboII 546 GAAGA GAAGA(8/7)
MflI 232 GGATCC [GA]^GATC[CT]
MroI 542 TCCGGA T^CCGGA
MslI 1006 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PstI 248 CTGCAG CTGCA^G
PvuII 902 CAGCTG CAG^CTG
RsaI 579 GTAC GT^AC
SexAI 581 ACCAGGT A^CC[AT]GGT
SfcI 248 CTGCAG C^T[GA][CT]AG
SspI 954 AATATT AAT^ATT
TaqI 1036 TCGA T^CGA
TthHB8I 1036 TCGA T^CGA
XcmI 986 CCATCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 232 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 233 GATCC GGATC(4/5)
232 GGATC
AvaII 142 GGACC G^G[AT]CC
660 GGACC
BanII 24 GGGCCC G[GA]GC[CT]^C
1015 GAGCCC
BsaJI 93 CCCAGG C^C[ACGT][ACGT]GG
395 CCGTGG
BsiYI 94 CCAGGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
941 CCACAAGCAGG
BslI 94 CCAGGAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
941 CCACAAGCAGG
DpnII 233 GATC ^GATC
616 GATC
EaeI 793 CGGCCA [CT]^GGCC[GA]
972 CGGCCA
FauI 82 GCGGG CCCGC(4/6)
139 GCGGG
HinfI 706 GATTC G^A[ACGT]TC
1065 GATTC
MboI 233 GATC ^GATC
616 GATC
NdeII 233 GATC ^GATC
616 GATC
PpuMI 141 GGGACCT [GA]G^G[AT]CC[CT]
659 GGGACCT
Sau3AI 233 GATC ^GATC
616 GATC
TfiI 706 GATTC G^A[AT]TC
1065 GATTC
Tsp509I 341 AATT ^AATT
463 AATT
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeIII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
TspRI