Restriction map for
pFN21AE1566
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MflI MluI MroI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 606 GGTACC G^GTACC
AccII 497 CGCG CG^CG
AcyI 914 GACGCC G[GA]^CG[CT]C
AlwI 1198 GATCC GGATC(4/5)
ApaI 50 GGGCCC GGGCC^C
Asp718I 606 GGTACC G^GTACC
AvaI 36 CCCGGG C^[CT]CG[GA]G
BanI 606 GGTACC G^G[CT][GA]CC
BsaAI 619 TACGTA [CT]AC^GT[GA]
BsaHI 914 GACGCC G[GA]^CG[CT]C
BsmAI 1131 GTCTC GTCTC(1/5)
BsoBI 36 CCCGGG C^[CT]CG[GA]G
Bsp1407I 840 TGTACA T^GTACA
BsrGI 840 TGTACA T^GTACA
BstUI 497 CGCG CG^CG
Cac8I 864 GCCTGC GC[ACGT]^[ACGT]GC
DpnII 1198 GATC ^GATC
DsaI 1222 CCGTGG C^C[GA][CT]GG
EcoNI 535 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 229 GATATC GAT^ATC
EcoT14I 200 CCTTGG C^C[AT][AT]GG
EcoT22I 1411 ATGCAT ATGCA^T
Hin1I 914 GACGCC G[GA]^CG[CT]C
HphI 462 GGTGA GGTGA(8/7)
KpnI 606 GGTACC GGTAC^C
MboI 1198 GATC ^GATC
MseI 1045 TTAA T^TAA
MslI 477 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 497 CGCG CG^CG
NdeII 1198 GATC ^GATC
NsiI 1411 ATGCAT ATGCA^T
Sau3AI 1198 GATC ^GATC
SmaI 36 CCCGGG CCC^GGG
SnaBI 619 TACGTA TAC^GTA
SspBI 840 TGTACA T^GTACA
SspI 1075 AATATT AAT^ATT
StuI 862 AGGCCT AGG^CCT
StyI 200 CCTTGG C^C[AT][AT]GG
Tru9I 1045 TTAA T^TAA
Tsp45I 1372 GTGAC ^GT[GC]AC
XbaI 435 TCTAGA T^CTAGA
XmaCI 36 CCCGGG C^CCGGG
XmaI 36 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 967 GGACC G^G[AT]CC
1215 GGACC
BalI 203 TGGCCA TGG^CCA
1058 TGGCCA
BsmFI 550 GGGAC GGGAC(10/14)
530 GTCCC
BstAPI 12 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
175 GCAAGTGTTGC
HapII 37 CCGG C^CGG
48 CCGG
HgaI 914 GACGC GACGC(5/10)
594 GCGTC
HpaII 37 CCGG C^CGG
48 CCGG
MluNI 203 TGGCCA TGG^CCA
1058 TGGCCA
MscI 203 TGGCCA TGG^CCA
1058 TGGCCA
MspI 37 CCGG C^CGG
48 CCGG
PpuMI 966 AGGACCT [GA]G^G[AT]CC[CT]
1214 AGGACCC
PstI 10 CTGCAG CTGCA^G
217 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MnlI MvaI MwoI NciI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp509I TspRI TthHB8I