Restriction map for
pFN21AE1379
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaJI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MluI MroI MseI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 28 ACATGT A^C[GA][CT]GT
BalI 173 TGGCCA TGG^CCA
BamHI 506 GGATCC G^GATCC
BanII 361 GAGCTC G[GA]GC[CT]^C
BbrPI 720 CACGTG CAC^GTG
BbsI 21 GAAGAC GAAGAC(2/6)
BciVI 323 GTATCC GTATCC(6/5)
BcnI 409 CCGGG CC^[GC]GG
BpuAI 21 GAAGAC GAAGAC(2/6)
BsaAI 720 CACGTG [CT]AC^GT[GA]
BseRI 289 CTCCTC GAGGAG(10/8)
BsgI 697 GTGCAG GTGCAG(16/14)
BsmFI 538 GTCCC GGGAC(10/14)
Bsp1407I 731 TGTACA T^GTACA
BspLU11I 28 ACATGT A^CATGT
BsrBI 510 CCGCTC CCGCTC(-3/-3)
BsrDI 687 CATTGC GCAATG(2/0)
BsrFI 523 GCCGGT [GA]^CCGG[CT]
BsrGI 731 TGTACA T^GTACA
BstAPI 35 GCACCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 506 GGATCC [GA]^GATC[CT]
Cfr10I 523 GCCGGT [GA]^CCGG[CT]
FauI 199 GCGGG CCCGC(4/6)
HinfI 738 GAGTC G^A[ACGT]TC
MaeII 721 ACGT A^CGT
MfeI 69 CAATTG C^AATTG
MflI 506 GGATCC [GA]^GATC[CT]
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
MunI 69 CAATTG C^AATTG
NciI 409 CCGGG CC^[GC]GG
NspI 28 ACATGT [GA]CATG^[CT]
PleI 738 GAGTC GAGTC(4/5)
PmaCI 720 CACGTG CAC^GTG
PmlI 720 CACGTG CAC^GTG
PshAI 219 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 445 CTGCAG CTGCA^G
SacI 361 GAGCTC GAGCT^C
SfcI 445 CTGCAG C^T[GA][CT]AG
SspBI 731 TGTACA T^GTACA
Tsp509I 70 AATT ^AATT
XhoII 506 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 346 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
546 CAGCTGCTG
AocI 158 CCTCAGG CC^T[ACGT]AGG
657 CCTGAGG
Aor51HI 395 AGCGCT AGC^GCT
430 AGCGCT
AvaII 438 GGACC G^G[AT]CC
537 GGTCC
BanI 34 GGCACC G^G[CT][GA]CC
163 GGCACC
BpmI 307 CTGGAG CTGGAG(16/14)
748 CTGGAG
BsaI 584 GAGACC GGTCTC(1/5)
637 GAGACC
BsrI 220 ACTGG ACTGG(1/-1)
488 ACTGG
BstXI 428 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
459 CCACTGCTGTGG
Bsu36I 158 CCTCAGG CC^T[ACGT]AGG
657 CCTGAGG
DdeI 159 CTCAG C^T[ACGT]AG
658 CTGAG
EaeI 173 TGGCCA [CT]^GGCC[GA]
528 TGGCCG
Eco47III 395 AGCGCT AGC^GCT
430 AGCGCT
Eco81I 158 CCTCAGG CC^T[ACGT]AGG
657 CCTGAGG
HaeII 395 AGCGCT [GA]GCGC^[CT]
430 AGCGCT
HapII 409 CCGG C^CGG
524 CCGG
HgaI 120 GACGC GACGC(5/10)
255 GCGTC
HpaII 409 CCGG C^CGG
524 CCGG
MspI 409 CCGG C^CGG
524 CCGG
SfaNI 113 GCATC GCATC(5/9)
623 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BmyI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SmlI
TseI Tsp45I TspRI